A5021594300- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Plant Pathogens and Fungal Diseases
- Polysaccharides and Plant Cell Walls
- Studies on Chitinases and Chitosanases
- Phytochemistry and Biological Activities
- Plant-Microbe Interactions and Immunity
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
- Food composition and properties
- Enzyme-mediated dye degradation
- Legume Nitrogen Fixing Symbiosis
- Mycorrhizal Fungi and Plant Interactions
- Polyamine Metabolism and Applications
- Fungal Biology and Applications
- Polysaccharides Composition and Applications
- Gut microbiota and health
- Biochemical and biochemical processes
- Advanced Cellulose Research Studies
- Microbial metabolism and enzyme function
Chalmers University of Technology
2017-2024
Wallenberg Wood Science Center
2017-2024
University of Technology
2021
University of Gothenburg
2019
Norwegian University of Life Sciences
2016-2017
KTH Royal Institute of Technology
2011-2016
AlbaNova
2011-2016
Canada's Michael Smith Genome Sciences Centre
2016
University of British Columbia
2016
Chitin is the second most abundant polysaccharide on earth and as such a great target for bioconversion applications. The phylum Bacteroidetes one of nature's ubiquitous bacterial lineages essential in global carbon cycle with many members being highly efficient degraders complex carbohydrates. However, despite their specialist reputation carbohydrate conversion, mechanisms degrading recalcitrant crystalline polysaccharides chitin cellulose are hitherto unknown.Here we describe complete...
Reflecting the diverse chemistry of plant cell walls, microorganisms that degrade these composite structures synthesize an array glycoside hydrolases. These enzymes are organized into sequence-, mechanism-, and structure-based families. Genomic data have shown several organisms wall contain a large number genes encoding family 43 (GH43) Here we report biochemical properties GH43 saprophytic soil bacterium, Cellvibrio japonicus, human colonic symbiont, Bacteroides thetaiotaomicron. The show...
Summary The degradation of plant biomass by saprophytes is an ecologically important part the global carbon cycle, which has also inspired a vast diversity industrial enzyme applications. xyloglucans ( XyGs ) constitute family ubiquitous and abundant cell wall polysaccharides, yet enzymology XyG saccharification poorly studied. Here, we present identification molecular characterization complex genetic locus that required for xyloglucan utilization model saprophyte C ellvibrio japonicus . In...
The thermophilic biomass-degrader Malbranchea cinnamomea exhibits poor growth on cellulose but excellent hemicelluloses as the sole carbon source. This is surprising considering that its genome encodes eight lytic polysaccharide monooxygenases (LPMOs) from auxiliary activity family 9 (AA9), enzymes known for their high potential in accelerating depolymerization. We characterized four of (M. AA9s) McAA9s, namely, McAA9A, McAA9B, McAA9F, and McAA9H, to gain a deeper understanding about roles...
The desire for improved methods of biomass conversion into fuels and feedstocks has re-awakened interest in the enzymology plant cell wall degradation. complex polysaccharide xyloglucan is abundant matter, where it may account up to 20% total primary carbohydrates. Despite this, few studies have focused on saccharification, which requires a consortium enzymes including endo-xyloglucanases, α-xylosidases, β-galactosidases α-L-fucosidases, among others. In present paper, we show...
The human gastrointestinal tract harbours myriad bacterial species, collectively termed the microbiota, that strongly influence health. Symbiotic members of our microbiota play a pivotal role in digestion complex carbohydrates are otherwise recalcitrant to assimilation. Indeed, intrinsic polysaccharide-degrading enzyme repertoire is limited various starch-based substrates; more polysaccharides demand microbial degradation. Select Bacteroidetes responsible for degradation ubiquitous vegetable...
Abstract Glucuronoyl esterases (GEs) are α/β serine hydrolases and a relatively new addition in the toolbox to reduce recalcitrance of lignocellulose, biggest obstacle cost-effective utilization this important renewable resource. While biochemical structural characterization GEs have progressed greatly recently, there yet been no mechanistic studies shedding light onto rate-limiting steps relevant for biomass conversion. The bacterial GE Ot CE15A possesses classical distinctive catalytic...
Lignocellulose is a renewable but complex material exhibiting high recalcitrance to enzymatic hydrolysis, which attributed, in part, the presence of covalent linkages between lignin and polysaccharides plant cell wall. Glucuronoyl esterases from carbohydrate esterase family 15 (CE15) have been proposed as an aid reducing this by cleaving ester bonds found glucuronoxylan. In Bacteroidota phylum, some species organize genes related metabolism polysaccharide utilization loci (PULs) encode all...
Cellulose is the most abundant renewable polymer on earth, but its recalcitrance limits highly efficient conversion methods for energy-related and material applications. Though microbial cellulose has been studied decades, recent advances showcased that large knowledge gaps still exist. Bacteria of phylum Bacteroidetes are regarded as carbohydrate metabolizers, species limited to (semi)soluble glycans. A few species, including soil bacteria C. hutchinsonii S. myxococcoides , specialists,...
Galactoglucomannan (GGM) is the most abundant hemicellulose in softwood, and consists of a backbone mannose glucose units, decorated with galactose acetyl moieties. GGM can be hydrolyzed into fermentable sugars, or used as polymer films, gels, food additives. Endo-β-mannanases, which found glycoside hydrolase families 5 26, specifically cleave mannan shorter oligosaccharides. Information on activity specificity different mannanases complex acetylated substrates still lacking. The aim this...
Lignocellulose is highly recalcitrant to enzymatic deconstruction, where the recalcitrance primarily results from chemical linkages between lignin and carbohydrates. Glucuronoyl esterases (GEs) carbohydrate esterase family 15 (CE15) have been suggested play key roles in reducing lignocellulose by cleaving covalent ester bonds found glucuronoxylan. However, only a limited number of GEs biochemically characterized structurally determined date, limiting our understanding these enzymes their...
Thermophilic fungi can represent a rich source of industrially relevant enzymes. Here, 105 fungal strains capable growing at 50 °C and pH 2.0 were isolated from compost decaying plant matter. Maximum growth temperatures the in range to 60 °C. Sequencing internal transcribed spacer (ITS) regions indicated that 78 belonged 12 species Ascomycota 3 Zygomycota, while no fungus Basidiomycota was detected. The remaining 27 could not be reliably assigned any known species. Phylogenetically, they...
The gut microbiota plays a central role in human health by enzymatically degrading dietary fiber and concomitantly excreting short chain fatty acids that are associated with manifold benefits. polysaccharide xylan is abundant but noncarbohydrate decorations hinder efficient cleavage glycoside hydrolases (GHs) need to be addressed carbohydrate esterases (CEs). Enzymes from esterase families 1 6 (CE1 6) perform key roles degradation removing feruloyl acetate decorations, yet little known about...
Abstract Background Biomass-degrading enzymes with improved activity and stability can increase substrate saccharification make biorefineries economically feasible. Filamentous fungi are a rich source of carbohydrate-active (CAZymes) for biomass degradation. The newly isolated LPH172 strain the thermophilic Ascomycete Thielavia terrestris has been shown to possess high xylanase cellulase activities tolerate low pH temperatures. Here, we aimed illuminate lignocellulose-degrading machinery...
Tannins are secondary metabolites that enriched in the bark, roots, and knots trees known to hinder microbial attack. The biological degradation of water-soluble gallotannins, such as tannic acid, is initiated by tannase enzymes (EC 3.1.1.20), which esterases able liberate gallic acid from aromatic-sugar complexes. However, only few tannases have previously been studied detail. Here, for first time, we biochemically structurally characterize three a single organism, anaerobic bacterium...
Abstract Carbohydrate-binding modules (CBMs) are non-catalytic proteins found appended to carbohydrate-active enzymes. Soil and marine bacteria secrete such enzymes scavenge nutrition, they often use CBMs improve reaction rates retention of released sugars. Here we present a structural functional analysis the recently established CBM family 92. All analysed bind preferentially β−1,6-glucans. This contrasts with diversity predicted substrates among attached CBM92 domains. We crystal...
The metabolism of the storage polysaccharides glycogen and starch is vital importance to organisms from all domains life. In bacteria, utilization these α-glucans requires concerted action a variety enzymes, including glycoside hydrolases, phosphorylases, transglycosylases. particular, transglycosylases hydrolase family 13 (GH13) GH77 play well established roles in α-glucan side chain (de)branching, regulation oligo- polysaccharide length, formation cyclic dextrans. Here, we present...
Genome and transcriptome sequencing has greatly facilitated the understanding of biomass-degrading mechanisms in a number fungal species. The information obtained enables investigation discovery genes encoding proteins involved plant cell wall degradation, which are crucial for saccharification lignocellulosic biomass second-generation biorefinery applications. thermophilic fungus
Plant biomass is an abundant and renewable carbon source that recalcitrant towards both chemical biochemical degradation. Xylan the second most polysaccharide in after cellulose, it possesses a variety of carbohydrate substitutions non-carbohydrate decorations which can impede enzymatic degradation by glycoside hydrolases. Carbohydrate esterases are able to cleave ester-linked thereby improve accessibility xylan backbone hydrolases, thus improving process. Enzymes comprising multiple...