A5076227403- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Catalysis for Biomass Conversion
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Cardiac electrophysiology and arrhythmias
- Pharmacogenetics and Drug Metabolism
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Anodic Oxide Films and Nanostructures
- Bacterial Genetics and Biotechnology
- Steroid Chemistry and Biochemistry
- Polysaccharides Composition and Applications
- Cardiomyopathy and Myosin Studies
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Icing and De-icing Technologies
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Antibiotic Resistance in Bacteria
- Ion channel regulation and function
- Antibiotics Pharmacokinetics and Efficacy
University of Chinese Academy of Sciences
2025
Tianjin Institute of Industrial Biotechnology
2018-2025
Chinese Academy of Sciences
2014-2025
Nankai University
2016-2023
State Key Laboratory of Medicinal Chemical Biology
2019-2023
University of Copenhagen
2021-2022
Howard Hughes Medical Institute
1994
Hydroxylation of C25 C–H bonds (referring to sterols) is great importance in vivo for metabolizing sterols and vitamin Ds. The biocatalytic hydroxylation restricted by the selectivity activity enzymes due inertness these bulky compounds. Herein, we employed fungal unspecific peroxygenase from Agrocybe aegerita (AaeUPO) as catalyst develop efficient selective AaeUPO variants through protein engineering. After three rounds evolution using semirational design, 2 variants, G195A/G241V/G318V...
Exosomes have recently emerged as some of the most promising biomarkers for disease diagnosis. Due to their small sizes and composition heterogeneity, exosomes are difficult detect by currently available platforms. Here, we report a pH-mediated assembly system that converts single nanosized into microsized clusters, which can be directly analyzed conventional flow cytometry (FCM), breaking through size limit exosome analysis. We demonstrated clinical utility clustering profiling exosomal...
Circulating tumor microenvironment-derived extracellular vesicles (cTME-EVs) are gaining considerable traction in cancer research and liquid biopsy. However, the study of cTME-EVs is largely limited by dearth a general isolation technique to selectively enrich from biological fluids for downstream analysis. In this work, we broke through dilemma presenting double-switch pH-low insertion peptide (D-S pHLIP) system exclusively harvest blood serum mouse models. This D-S pHLIP consists highly...
Abstract Glucuronoyl esterases (GEs) are α/β serine hydrolases and a relatively new addition in the toolbox to reduce recalcitrance of lignocellulose, biggest obstacle cost-effective utilization this important renewable resource. While biochemical structural characterization GEs have progressed greatly recently, there yet been no mechanistic studies shedding light onto rate-limiting steps relevant for biomass conversion. The bacterial GE Ot CE15A possesses classical distinctive catalytic...
Abstract Exosomes, ranging from 30–150 nm in diameter, have emerged as promising non‐invasive biomarkers for the diagnosis and prognosis of numerous diseases. However, current research on exosomes is largely restricted by lack an efficient method to isolate real samples. Herein, first stimuli‐mediated enrichment purification system selectively efficiently extract clinical plasma high‐throughput profiling exosomal mRNAs cancer presented. This novel isolation relies specific installation...
Searching for viable strategies to accelerate the catalytic cycle of glycoside hydrolase family 7 (GH7) cellobiohydrolase I (CBHI)—the workhorse cellulose-degrading enzymes, we have performed a total 12-μs molecular dynamics simulations on GH7 CBHI, which brought light new mechanism cellobiose expulsion, coined "claw-arm" action. The loop flanking product binding site plays role flexible "arm" extending toward cellobiose, and residue Thr389 this acts as "claw" that captures cellobiose. Five...
We investigated the nature and structural requirements for Ca2+-dependent inactivation of cardiac L-type Ca2+ channel. Investigation subunit indicates that interaction α1 with ancillary subunits, especially β subunit, is important this property. Replacement putative cytoplasmic regions skeletal muscle counterparts eliminates inactivation, indicating site regulated by resides in region subunit. Deletion carboxy-terminal does not eliminate property, suggesting modulation protein kinase A may...
Carbapenemase-producing bacteria (CPB) stand as the most dangerous "superbugs" in clinic. Rapid point-of-care (POC) detection of CPB clinical samples is key to timely and effective infection management. We herein report first ultrasensitive chromogenic probe that allows direct POC sputum at a sample-to-result time less than 15 min. This modularly designed by conjugating carbapenem core with benzene derivative bearing an electronegativity-tunable substituent. Unexpectedly high sensitivity was...
Branching enzymes (BEs) confer to α-glucans, the primary energy-storage reservoir in nature, a variety of features, like slow digestion. The full catalytic cycle BEs can be divided six steps, namely two covalent steps involving glycosylation and transglycosylation, four noncatalytic substrate binding transfers (SBTs). Despite ever-growing wealth biochemical structural information on BEs, clear mechanistic insights into SBTs from an industrial-performance perspective are still missing. Here,...
Cellobiohydrolases (CBHs) are the most significant cellulose-degrading enzymes, performance of which determines cost-effective utilization renewable lignocellulosic resources. Most engineering strategies for improving CBH hydrolysis currently focused on accelerating noncatalytic enzyme–substrate dissociation by increasing flexibility eight substrate-enclosing loops (SELs), does not take catalysis into account or even deteriorates it. Here, in model Trichoderma reesei CBHI, we identified a...
β-mannanase is a key enzyme for hydrolyzing mannan, major constituent of hemicellulose, which the second most abundant polysaccharide in nature. Different structural domains greatly affect its biochemical characters and catalytic efficiency. However, effects linker carbohydrate-binding module (CBM) on from Trichoderma reesei (Man1) have not yet been fully described. The present study aimed to determine influence different expression efficiency, characteristics hemicellulosic deconstruction...
An experimental and computational study on a strategy for biomass degradation in biofuel production, pretreating cellulases with hydrophobins.
Temperate bacteriophages can switch between two life cycles following infection of a host bacterium: the lytic or lysogenic cycle. The choice these is controlled by bistable genetic switch. We investigated lactococcal temperate bacteriophage, TP901‐1, which regulatory proteins, Clear 1 (CI) repressor and modulator repression (MOR) antirepressor. CI consists DNA‐binding N‐terminal domain C‐terminal responsible for oligomerization, connected flexible interdomain linker. Full‐length hexameric,...
Abstract Lignocellulosic biomass can be converted into biofuels and biochemicals, through a synergetic degradation by cellulases. The underlying cooperative mechanism is of paramount importance for the development efficient enzyme cocktails. synergy between exo–lytic cellulases, however, still remains poorly understood. In present contribution, we decipher synergism investigation enzyme–enzyme interactions (EEIs) two cellulases–– Talaromyces emersonii Cel7A ( Te Cel7A) Trichoderma reesei...