A5046472794- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Carbohydrate Chemistry and Synthesis
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Asymmetric Synthesis and Catalysis
- Advanced biosensing and bioanalysis techniques
- Toxin Mechanisms and Immunotoxins
- Chemical Synthesis and Analysis
- Synthetic Organic Chemistry Methods
- Hepatitis C virus research
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- Computational Drug Discovery Methods
- Click Chemistry and Applications
- Porphyrin and Phthalocyanine Chemistry
- Enzyme Catalysis and Immobilization
- Biochemical and Structural Characterization
- Cancer Research and Treatments
- HIV/AIDS drug development and treatment
- Viral gastroenteritis research and epidemiology
- Diet, Metabolism, and Disease
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
State Key Laboratory of Medicinal Chemical Biology
2012-2025
Nankai University
2016-2025
Industrial Technology Research Institute
2024
Life University
2017
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2015
University Hospital Frankfurt
2011
GlaxoSmithKline (United States)
2011
Goethe Institute
2011
Goethe University Frankfurt
2011
Scripps Research Institute
2005-2010
Due to its simplicity, versatility, and high efficiency, the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 technology has emerged as one of most promising approaches for treatment a variety genetic diseases, including human cancers. However, further translation CRISPR/Cas9 cancer gene therapy requires development safe efficient, highly specific delivery both Cas9 single guide RNA tumors. Here, novel core-shell nanostructure, liposome-templated hydrogel nanoparticles...
Fully unprotected peptide o-aminoanilides can be efficiently activated by NaNO2 in aqueous solution to furnish thioesters for use native chemical ligation. This finding enables the convergent synthesis of proteins from readily synthesizable as a new type crypto-thioesters. The practicality this approach is shown histone H2B five segments. Purification or solubilization tags, which are sometimes needed improve efficiency protein synthesis, incorporated into o-aminoanilide moiety, demonstrated...
Abstract Sortase‐mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production recombinant protein hydrazides. This process provides an alternative approach semisynthesis through use hydrazides as thioester surrogates. It also method C‐terminal modification functional units well preparation C‐to‐C fusion proteins.
A one-pot chemoenzymatic method for the synthesis of a variety new iminocyclitols from readily available, non-phosphorylated donor substrates has been developed. The utilizes recently discovered fructose-6-phosphate aldolase (FSA), which is functionally distinct known aldolases in its tolerance different as well acceptor substrates. Kinetic studies were performed with dihydroxyacetone (DHA), presumed endogenous substrate FSA, hydroxy acetone (HA) and 1-hydroxy-2-butanone (HB) substrates,...
Abstract Therapeutic tumor neoantigen vaccines have been widely studied given their good safety profile and ability to avoid central thymic tolerance. However, targeting antigen‐presenting cells (APCs) inducing robust neoantigen‐specific cellular immunity remain challenges. Here, a safe broad‐spectrum vaccine delivery system is proposed (GP‐Neoantigen) based on β ‐1,3‐glucan particles (GPs) derived from Saccharomyces cerevisiae coupling peptide antigens with GPs through convenient click...
It has been demonstrated that the two main subsets of peripheral γδ T cells, Vγ1 and Vγ4, have divergent functions in many diseases models. Recently, we reported Vγ4 cells played a protective role tumor immunity through eomesodermin-controlled mechanisms. However, precise roles immunity, especially whether any interaction with remain unknown. We this paper suppressed cell-mediated antitumor function both vitro vivo, suppression was cell contact independent. Using neutralizing anti-IL-4 Ab or...
Con A-induced fulminant hepatitis is a well-known animal model for acute liver failure. However, the role of γδ T cells in this undefined. In report, using TCR δ(-/-) mice, we demonstrated protective model. mice showed significantly decreased levels IL-17A and IL-17F A-treated tissue, reconstitution with wild-type (Wt), but not IL-17A(-/-), reduced hepatitis, strongly suggesting critical mediating effect cells. Interestingly, only Vγ4, Vγ1, exerted such effect. Furthermore, depletion NKT...
Biological uses of photosensitizers in photodynamic therapy (PDT) often suffer from a lack tumor selectivity; strategy based on molecule-targeted cancer therapies could provide promising solution.
The NS5B polymerase is one of the most attractive targets for developing new drugs to block Hepatitis C virus (HCV) infection. We describe discovery novel potent HCV inhibitors by employing a virtual screening (VS) approach, which based on random forest (RB-VS), e-pharmacophore (PB-VS), and docking (DB-VS) methods. In RB-VS stage, after feature selection, model with 16 descriptors was used. PB-VS six energy-based pharmacophore (e-pharmacophore) models from different crystal structures...
Background/Objectives: Finotonlimab (SCTI10A) is a humanized anti-PD-1 antibody tested in Phase III trials for several solid tumor types. Methods: This study characterized the vitro and vivo efficacy, Fc-mediated effector function, non-clinical PK/PD properties of finotonlimab. Results: The results demonstrated that finotonlimab effective stimulating human T cell function exhibits marked antitumor efficacy using both PD-1-humanized PBMC-reconstructed mouse models. Additionally, exhibited...
Abstract Dihydroxyacetone phosphate (DHAP)‐dependent aldolases have been widely used for the organic synthesis of unnatural sugars or derivatives. The practicality using DHAP‐dependent is limited by their strict substrate specificity and high cost instability DHAP. Here we report that aldolase L ‐rhamnulose 1‐phosphate (RhaD) accepts dihydroxyacetone (DHA) as a donor in presence borate buffer, presumably reversible situ formation DHA ester. reaction appears to be irreversible, with products...
Flexible yet efficient: Sialic acids such as L-N-acetylneuraminic acid (see picture) can be synthesized in only three steps by 1) vinylation of an aldose through a modified Petasis coupling reaction, 2) 1,3-dipolar cycloaddition with nitrone to construct isoxazolidine ring, and 3) base-catalyzed β elimination/ring opening the generate γ-hydroxy-α-keto acid. Supporting information for this article is available on WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z601555_s.pdf or from...
A two-step method has been developed to synthesize several biologically important iminocyclitols in ca. 44−60% yields by using Petasis-type condensation. The is very general and operationally simple, affording a series of from easily available sugar derivatives. Unexpected diastereoselectivities are observed, suggesting that the condensation may proceed through five- or six-membered cyclic iminium ion intermediate.
Pyropheophorbide-a (Pyro) is a highly promising photosensitizer for tumor photodynamic therapy (PDT), although its very limited tumor-accumulation ability seriously restricts clinical applications. A higher accumulation of photosensitizers important the treatment deeply seated and larger tumors. The conjugation Pyro with tumor-homing peptide ligands could be useful strategy to optimize physical properties Pyro. Herein, we reported our studies on cyclic cRGDfK (cRGD) peptide, an integrin...
Human immunodeficiency virus type-1 and hepatitis C (HIV/HCV) coinfection occurs when a patient is simultaneously infected with both human (HIV-1) (HCV), which common today in certain populations. However, the treatment of challenge because special considerations needed to ensure hepatic safety avoid drug-drug interactions. Multitarget inhibitors less toxicity may provide promising therapeutic strategy for HIV/HCV coinfection. identification one molecule that acts on multiple targets by...
In December 2019, the world awoke to a new betacoronavirus strain named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Betacoronavirus consists of A, B, C and D subgroups. Both SARS-CoV SARS-CoV-2 belong subgroup B. present study, we divided B into SARS1 SARS2 classes by six key insertions deletions (InDels) in genomes, identified recently detected strains RmYN02 as recombinant across classes, which has potential generate with similar risk SARS-CoV-2. By analyzing genomic...
An efficient l -3-deoxy- manno -2-octulosonic acid ( -KDO) aldolase was created by directed evolution from the Escherichia coli d -Neu5Ac N -acetylneuraminic acid, -sialic acid) aldolase. Five rounds of error-prone PCR and iterative screening were performed with sampling 10 3 colonies per round. The specificity constant k cat / K m ) unnatural sugar -KDO is improved to a level equivalent wild-type for its natural substrate, -Neu5Ac. final evolved enzyme exhibits >1,000-fold ratio [...
Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus-associated malignancy occurring at high incidence in Southeast Asia and southern China.In spite of the good response to radio-and chemo-therapy early stage, resistance recurrence develop NPC patients advanced setting.Cancer stem cells (CSCs) play important role drug cancer recurrence.Here we report that lovastatin, a natural compound lipophilic statin has already been used clinic treat hypercholesterolemia, inhibited CSC properties...
Highly hydrophilic modification enhances the selectivity of targeted photosensitizer delivery.
Phthalocyanines (Pcs) are a group of promising photosensitizers for use in photodynamic therapy (PDT). However, their extremely low solubility and strong tendency to aggregate aqueous solution greatly restrict application. Conjugation Pc macrocycles with peptide ligands could be very useful strategy optimize the physical properties Pcs not only by increasing water reducing aggregation but also endowing conjugates tumor-targeting capability. To develop highly potent tumor PDT, we prepared new...