A5039629749- RNA Research and Splicing
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- Carbohydrate Chemistry and Synthesis
- Immune cells in cancer
- Radiomics and Machine Learning in Medical Imaging
- HIV Research and Treatment
- Cancer-related molecular mechanisms research
- Cholesterol and Lipid Metabolism
- AI in cancer detection
- Melanoma and MAPK Pathways
- Advanced Proteomics Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Lipid Membrane Structure and Behavior
- Kruppel-like factors research
- Cancer, Lipids, and Metabolism
- Parathyroid Disorders and Treatments
- Protein Kinase Regulation and GTPase Signaling
- Cancer Genomics and Diagnostics
- Remote Sensing and Land Use
- Ubiquitin and proteasome pathways
- Soil Carbon and Nitrogen Dynamics
- Ion channel regulation and function
- Peatlands and Wetlands Ecology
China West Normal University
2018-2024
Hebei University of Science and Technology
2017-2024
Scripps Research Institute
2009-2022
European Bioinformatics Institute
2019-2022
Xi'an Jiaotong University
2022
Sichuan University
2022
Sichuan Academy of Agricultural Sciences
2022
Nanjing University of Chinese Medicine
2019-2020
Leiden University
2020
Peking University
2004-2019
The role of Fat Mass and Obesity-associated protein (FTO) its substrate N6-methyladenosine (m6A) in mRNA processing adipogenesis remains largely unknown. We show that FTO expression m6A levels are inversely correlated during adipogenesis. depletion blocks differentiation only catalytically active restores Transcriptome analyses combination with m6A-seq revealed gene splicing grouped genes regulated by FTO. M6A is enriched exonic regions flanking 5′- 3′-splice sites, spatially overlapping...
Significance Membrane proteins (MPs) perform a variety of essential cellular functions, account for about one-third encoded in genomes, and comprise more than one-half human drug targets. High-resolution structures are to understand the underlying molecular mechanisms MPs facilitate structure-based design efforts. Detergents indispensible solubilization MPs, but they tend destabilize often impede growth well-ordered protein crystals. We describe class structurally unique detergents,...
Abstract Wnt/β‐catenin signalling is widely implicated in embryogenesis, tissue homeostasis and tumorigenesis. The key event Wnt activation β‐catenin accumulation, which controlled by both its production degradation. However, much more emphasis has been placed on the understanding of Here, we show that synthesis protein, requires a group serine/arginine‐rich splicing factors (SRSF), also contributes to tumorigenic activity. Overexpression SRSF1 SRSF9 promote accumulation via recruitment mRNA...
Made for each other: Combinatorial alanine substitution of active site residues in a thermostable cytochrome P450BM3 variant was used to generate an enzyme that is with large substrates. Selective hydroxylation methoxymethylated monosaccharides, alkaloids, and steroids thus made possible (see Scheme). This approach could be useful improving the activity enzymes show only limited larger
Succinate-ubiquinone oxidoreductase (SQR) is an attractive target for fungicide discovery. Herein, we report the discovery of novel SQR inhibitors using a pharmacophore-linked fragment virtual screening approach, new drug design method developed in our laboratory. Among newly designed compounds, compound 9s was identified as most potent inhibitor with Ki value 34 nM against porcine SQR, displaying approximately 10-fold higher potency than that commercial control penthiopyrad. Further...
Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range biological processes, but the synthesis these compounds is challenging because their complex structures. We have developed chemoenzymatic method for regioselective deprotection monosaccharide substrates using engineered Bacillus megaterium cytochrome P450 (P450(BM3)) demethylases provides highly efficient means to access valuable intermediates, which can be converted substituted...
Follistatin-like protein 1 (FSTL-1) is overexpressed in a number of inflammatory conditions characterized by elevated IL-1β. Here, we found that FSTL-1 serum concentration was increased threefold patients with bacterial sepsis and fourfold following administration LPS to mice. To test the contribution IL-1β secretion, WT FSTL-1-deficient mice were injected LPS. While induced sera mice, it low or undetectable Monocytes/macrophages, key source IL-1β, do not normally express FSTL-1. However,...
Serine/arginine-rich (SR) proteins are a family of important splicing factors, which involved in multiple aspects RNA processing, including splicing, mRNA nuclear export, stability and translation. Previous studies have identified number SR that exhibit abnormal expression various tumor types. In the present study, function serine/arginine-rich factor 7 (SRSF7) were investigated colon lung cancer. Using tissue immunohistochemistry, it was observed SRSF7 overexpressed cancer tissues. As role...
A two-step method has been developed to synthesize several biologically important iminocyclitols in ca. 44−60% yields by using Petasis-type condensation. The is very general and operationally simple, affording a series of from easily available sugar derivatives. Unexpected diastereoselectivities are observed, suggesting that the condensation may proceed through five- or six-membered cyclic iminium ion intermediate.
The diagnosis of cancer is typically based on histopathological assessment tissue sections, and supplemented by genetic other molecular tests 1–6 . Modern computer vision algorithms have high diagnostic accuracy potential to augment histopathology workflows 7–9 Here we use deep transfer learning quantify patterns across 17,396 hematoxylin eosin (H&E) stained slide images from 28 types correlate these with matched genomic, transcriptomic survival data. This approach accurately classifies...
AbstractWnt/β-catenin signaling is fundamental in embryogenesis and tissue homeostasis metazoans. Upon Wnt stimulation, cognate coreceptors LRP5 LRP6 ([LRP5/6] low-density lipoprotein receptor-related proteins 5 6) are activated via phosphorylation at key residues. Although several kinases have been implicated, the LRP5/6 activation mechanism remains unclear. Here, we report that transmembrane protein 198 (TMEM198), a previously uncharacterized seven-transmembrane protein, able to...
Collagen is the building component of temporomandibular joint (TMJ) discs and often affected by inflammation in disorders. The macromechanical properties collagen are deteriorated chronic inflammation. However, mechanism which influences disc function remains unknown. relationship between ultrastructure nanomechanical inflamed should be clarified. Seven-week-old female Sprague–Dawley rats were randomly divided into two groups. Chronic TMJ was induced intra-articular injection complete...
To generate new mechanistic hypotheses on the pathogenesis and disease progression of neuroHIV identify novel therapeutic targets to improve neuropsychological function in people with HIV, we investigated host genes pathway dysregulations associated brain HIV RNA load gene expression profiles frontal cortex, basal ganglia, white matter HIV+ patients. Pathway analyses showed that correlated all three regions were predominantly related inflammation, neurodegeneration, bioenergetics. directly...
Risk assessment of gastrointestinal stromal tumor (GIST) according to the AFIP/Miettinen classification and mutational profiling are major tools for patient management. However, depends heavily on mitotic counts, which is laborious sometimes inconsistent between pathologists. It has also been shown be imperfect in stratifying patients. Molecular testing costly time-consuming, therefore, not systematically performed all countries. New methods improve risk molecular predictions hence crucial...
The β-catenin-lymphoid enhancer factor (LEF) protein complex is the key mediator of canonical Wnt signaling and initiates target gene transcription upon ligand stimulation. In addition to β-catenin LEF themselves, many other proteins have been identified as necessary cofactors. Here we report that evolutionally conserved splicing transcriptional co-regulator, SKIP/SNW/NcoA62, forms a ternary with LEF1 HDAC1 mediates repression genes. Loss-of-function studies showed SKIP obligatory for...