A5004470364- RNA modifications and cancer
- HVDC Systems and Fault Protection
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Muscle Physiology and Disorders
- RNA Research and Splicing
Oslo University Hospital
2021-2023
University of Oslo
2021
University of Chinese Academy of Sciences
2013-2015
Beijing Institute of Genomics
2015
The role of Fat Mass and Obesity-associated protein (FTO) its substrate N6-methyladenosine (m6A) in mRNA processing adipogenesis remains largely unknown. We show that FTO expression m6A levels are inversely correlated during adipogenesis. depletion blocks differentiation only catalytically active restores Transcriptome analyses combination with m6A-seq revealed gene splicing grouped genes regulated by FTO. M6A is enriched exonic regions flanking 5′- 3′-splice sites, spatially overlapping...
Abstract Current N 6 -methyladenosine (m A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m A immunoprecipitation sequencing (picoMeRIP–seq) for studying in vivo single cells scarce cell types using standard laboratory equipment. We benchmark on titrations poly(A) embryonic stem zebrafish zygotes, mouse oocytes embryos.
Regulation of actomyosin dynamics by post-transcriptional modifications in cytoplasmic actin is still poorly understood. Here we demonstrate that dioxygenase ALKBH4-mediated demethylation a monomethylated site (K84me1) regulates actin–myosin interaction and actomyosin-dependent processes such as cytokinesis cell migration. ALKBH4-deficient cells display elevated K84me1 levels. Non-muscle myosin II only interacts with unmethylated its proper recruitment to depend on ALKBH4. ALKBH4...
Significance Dynamic deposition of the N6-methyladenosine (m 6 A) modification on messenger RNA (mRNA) regulates pluripotency in embryonic stem cells. Reports show that depletion m A abundances increases mRNA stability and lineage transcription factors (TFs) alike. If mRNAs these two TF groups become stabilized, it remains unclear how or commitment decision is implemented. Quantification TFs live at single-cell resolution over generations shows long-term preservation both priming. activates...