A5024011349- RNA modifications and cancer
- Reproductive Biology and Fertility
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Ovarian function and disorders
- Genetic Neurodegenerative Diseases
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Endometriosis Research and Treatment
- Metabolism and Genetic Disorders
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Electrowetting and Microfluidic Technologies
- Advanced Measurement and Metrology Techniques
- Endometrial and Cervical Cancer Treatments
- Advanced Surface Polishing Techniques
- Uterine Myomas and Treatments
- Advanced Machining and Optimization Techniques
- Renal and related cancers
- CRISPR and Genetic Engineering
- HVDC Systems and Fault Protection
- Prenatal Screening and Diagnostics
- Microfluidic and Bio-sensing Technologies
- Advanced MEMS and NEMS Technologies
- Genomics and Chromatin Dynamics
Oslo University Hospital
2014-2023
Newcastle University
2011-2014
Wellcome Centre for Mitochondrial Research
2014
Centre for Life
2010-2011
Abstract Current N 6 -methyladenosine (m A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m A immunoprecipitation sequencing (picoMeRIP–seq) for studying in vivo single cells scarce cell types using standard laboratory equipment. We benchmark on titrations poly(A) embryonic stem zebrafish zygotes, mouse oocytes embryos.
The early decline and loss of female fertility in humans other species represents an evolutionary paradox. Despite being born with a vast stock oocytes, females encounter exhaustion ovarian reserve sterility half way through their natural lives. Female reproductive ageing has been proposed to proceed as ongoing reserve, determined by remaining follicle number. However, despite extensive modelling, the respective contributions intra-, inter-, extra-ovarian signalling have not fully...
With increasing survival rates after treatment for cancer in prepubertal girls and women of reproductive age, an focus on quality life has emerged. Both irradiation cytotoxic drugs can be detrimental to future fertility, consequently several alternatives have been developed spare or restore fertility young females diagnosed with cancer. One these options is cryopreservation ovarian tissue before autotransplantation at a later time.We present the Norwegian experience 11 years practice...
ALKBH4, an AlkB homologue in the 2-oxoglutarate and Fe2+ dependent hydroxylase family, has previously been shown to regulate level of monomethylated lysine-84 actin thereby indirectly influences ability non-muscular myosin II bind filaments. ALKBH4 modulates fundamental processes including cytokinesis cell motility, its depletion is lethal during early preimplantation embryo stage. The aim this study was investigate effect deficiency a physiological context, using inducible Alkbh4 knockout...
Induced pluripotent stem cells (iPSCs) hold much promise in the quest for personalised cell therapies. However, persistence of founder mitochondrial DNA (mtDNA) mutations limits potential iPSCs development treatments mtDNA disease. This problem may be overcome by using oocytes containing healthy mtDNA, to induce somatic nuclear reprogramming. extent which persists following fusion with human is unknown. Here we show that transfer (NT) embryos contain very low levels mtDNA. In light a recent...
In this work, we present the characterisation of an electrothermally actuated microgripper that operates in both dry and liquid media, shows improved performance versus existing devices. The microgripper, fabricated a combination polymeric (SU8) conductive materials (Au), is able to produce displacements up 110 μm air 30 liquid. cases, voltage electrical power required minimal (less than 3 V 180 mW respectively) so both, high temperatures electrolysis, are prevented. Micromanipulation...