A5014264922- Prenatal Screening and Diagnostics
- Reproductive Biology and Fertility
- Genomic variations and chromosomal abnormalities
- Assisted Reproductive Technology and Twin Pregnancy
- Genetic Syndromes and Imprinting
- Chromosomal and Genetic Variations
- Congenital Anomalies and Fetal Surgery
- Pluripotent Stem Cells Research
- Renal and related cancers
- Gestational Trophoblastic Disease Studies
- IL-33, ST2, and ILC Pathways
- Parvovirus B19 Infection Studies
- Epigenetics and DNA Methylation
- Uterine Myomas and Treatments
- HVDC Systems and Fault Protection
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- Sperm and Testicular Function
- Endometrial and Cervical Cancer Treatments
- Reproductive Health and Technologies
- Ovarian function and disorders
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
Oslo University Hospital
2020-2023
Igenomix
2016-2018
Universitat de València
2016
California Institute for Regenerative Medicine
2014-2015
Parc Científic de la Universitat de València
2015
Stanford University
2014
Fundación Instituto Valenciano de Oncología
2014
Institute for Stem Cell Biology and Regenerative Medicine
2014
Instituto Valenciano de la Edificación
2014
What is the origin and composition of cell-free DNA in human embryo spent culture media? Cell-free from media represents a mix maternal embryonic DNA, mixture can be more complex for mosaic embryos. In 2016, ~300 000 embryos were chromosomally and/or genetically analyzed using preimplantation genetic testing aneuploidies (PGT-A) or monogenic disorders (PGT-M) before transfer into uterus. While progress techniques has enabled analysis full karyotype single cell with high sensitivity...
Aneuploidies are prevalent in the human embryo and impair proper development, leading to cell cycle arrest. Recent advances imaging molecular genetic analyses postulated as promising strategies unveil mechanisms involved aneuploidy generation. Here we combine time-lapse, complete chromosomal assessment single-cell RT-qPCR simultaneously obtain information from all cells that compose a until approximately eight-cell stage (n=85). Our data indicate status of aneuploid embryos (n=26), including...
How can we study the full transcriptome of endometrial stromal and epithelial cells at single-cell level?By compiling developing novel analytical tools for biopsy, tissue cryopreservation disaggregation, sorting, library preparation, RNA sequencing (RNA-seq) statistical data analysis.Although analyses from various biopsied tissues have been published recently, corresponding protocols human endometrium not described.The frozen-thawed biopsies were fluorescence-activated cell sorted (FACS) to...
Abstract Current N 6 -methyladenosine (m A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m A immunoprecipitation sequencing (picoMeRIP–seq) for studying in vivo single cells scarce cell types using standard laboratory equipment. We benchmark on titrations poly(A) embryonic stem zebrafish zygotes, mouse oocytes embryos.
A map of human embryo development that combines imaging, molecular, genetic and epigenetic data for comparisons to other species across pathologies would be greatly beneficial basic science clinical applications. Here, we compared mRNA protein expression key mediators DNA methylation histone modifications between mouse embryos, embryos from fertile/infertile couples, following growth factor supplementation. We observed individual are characterized by similarities distinct differences in...
(Abstracted from Human Reprod 2018;33:745–756) Chromosomal aneuploidy is common. It occurs in 20% to 80% of human embryos.
La methode de selection embryonnaire la plus commune en fecondation in vitro (FIV) repose sur les caracteristiques morphologiques et developpement embryonnaire. Cependant, le potentiel implantatoire des embryons humains reste faible pour certains groupes patients. Pour cette raison, l’evaluation viabilite par depistage d’aneuploidie pourrait ameliorer resultats FIV chez patients mauvais pronostic. Le “Comprehensive chromosome screening” (CGS) combine a d’autres techniques augmenter...