A5101775311- RNA modifications and cancer
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Genomics and Chromatin Dynamics
- Laser Applications in Dentistry and Medicine
- Genomics and Phylogenetic Studies
- Pain Mechanisms and Treatments
- Epigenetics and DNA Methylation
- Pesticide Residue Analysis and Safety
- Microbial Community Ecology and Physiology
- Spinal Cord Injury Research
- RNA Research and Splicing
- Gut microbiota and health
- Gastrointestinal motility and disorders
- Adipose Tissue and Metabolism
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Analytical chemistry methods development
- Hip disorders and treatments
- Cancer-related molecular mechanisms research
- Healthcare and Venom Research
- Vibrio bacteria research studies
- Tissue Engineering and Regenerative Medicine
- Aquaculture disease management and microbiota
- HVDC Systems and Fault Protection
The Ohio State University
2019-2025
Harbin Institute of Technology
2021-2025
University of Michigan
2023-2025
Shantou University Medical College
2024-2025
State Key Laboratory of Transducer Technology
2024-2025
Shanghai Institute of Microsystem and Information Technology
2024-2025
Shantou University
2024-2025
Shanghai Changzheng Hospital
2022-2024
Chinese Academy of Sciences
2021-2024
ShanghaiTech University
2024
<ns4:p><ns4:italic>Background: </ns4:italic>Given the demonstrated utility of Third Generation Sequencing [Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)] long reads in many studies, a comprehensive analysis comparison their data quality applications is high demand. <ns4:italic>Methods: </ns4:italic>Based on transcriptome sequencing from human embryonic stem cells, we analyzed multiple features PacBio ONT, including error pattern, length, mappability technical...
Abstract As one of the most intractable neurological diseases, spinal cord injury (SCI) often leads to permanent impairment in patients. Unfortunately, due complex pathological mechanisms and unique postinjury microenvironment, there is currently no way completely repair injured cord. In recent years, with rapid development tissue engineering technology, combination biomaterials medicine has provided a new idea for treating SCI. Here, we systematically summarize representative biomaterials,...
Junctophilin-2 (JP2) is a structural protein required for normal excitation-contraction (E-C) coupling. After cardiac stress, JP2 cleaved by the calcium ion-dependent protease calpain, which disrupts E-C coupling ultrastructural machinery and drives heart failure progression. We found that stress-induced proteolysis of liberates an N-terminal fragment (JP2NT) translocates to nucleus, binds genomic DNA, controls expression spectrum genes in cardiomyocytes. Transgenic overexpression JP2NT mice...
Abstract Current N 6 -methyladenosine (m A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m A immunoprecipitation sequencing (picoMeRIP–seq) for studying in vivo single cells scarce cell types using standard laboratory equipment. We benchmark on titrations poly(A) embryonic stem zebrafish zygotes, mouse oocytes embryos.
Van der Waals (vdW) ferromagnetic materials have emerged as a promising platform for the development of 2D spintronic devices. However, studies to date are restricted vdW with low Curie temperature (T
<ns4:p><ns4:italic>Background: </ns4:italic>Given the demonstrated utility of Third Generation Sequencing [Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)] long reads in many studies, a comprehensive analysis comparison their data quality applications is high demand. <ns4:italic>Methods: </ns4:italic>Based on transcriptome sequencing from human embryonic stem cells, we analyzed multiple features PacBio ONT, including error pattern, length, mappability technical...
Genome-wide chromatin accessibility and nucleosome occupancy profiles have been widely investigated, while the long-range dynamics remain poorly studied at single-cell level. Here, we present a new experimental approach, methyltransferase treatment followed by single-molecule long-read sequencing (MeSMLR-seq), for mapping of nucleosomes single DNA molecules thus achieve comprehensive-coverage characterization corresponding heterogeneity. MeSMLR-seq offers direct measurements both...
Abstract Spinal cord injury (SCI) represents a profound central nervous system affliction, resulting in irreversibly compromised daily activities and disabilities. SCI involves excessive inflammatory responses, which are characterized by the existence of high levels proinflammatory M1 macrophages, neuronal mitochondrial energy deficit, exacerbating secondary damage impeding axon regeneration. This study delves into mechanistic intricacies SCI, offering insights from perspectives neuroimmune...
Allele-specific expression (ASE) is a fundamental problem in studying gene regulation and diploid transcriptome profiles, with two key challenges: (i) haplotyping (ii) estimation of ASE at the isoform level. Existing analysis methods are limited by dependence on from laborious experiments or extra genome/family trio data. In addition, there lack for level analysis. We developed tool, IDP-ASE, full By innovative integration Third Generation Sequencing (TGS) long reads Second (SGS) short...
Abstract Sensory nerves are long being recognized as collecting units of various outer stimuli; recent advances indicate that the sensory nerve also plays pivotal roles in maintaining organ homeostasis. Here, this study shows orchestrates intervertebral disc (IVD) homeostasis by regulating its extracellular matrix (ECM) metabolism. Specifically, genetical denervation IVD results loss water preserve molecule chondroitin sulfate (CS), reduction CS bio‐synthesis gene synthase 1 (CHSY1)...
Abstract Although previous studies have shown that transposable elements (TEs) are conservatively activated to play key roles during early embryonic development, the details of zygotic TE activation (ZTA) remain poorly understood. Here, we employ long-read sequencing precisely identify only a small subset loci among numerous copies, allowing us map their hierarchical transcriptional cascades at single-locus and single-transcript level. Despite heterogeneity ZTA across family, subfamily,...
Intervertebral disc degeneration (IDD) has been identified as one of the predominant factors leading to persistent low back pain and disability in middle-aged elderly people. Dysregulation Prostaglandin E2 (PGE2) can cause IDD, while low-dose celecoxib maintain PGE2 at physiological level activate skeletal interoception. Here, nano fibers have extensively used treatment novel polycaprolactone (PCL) loaded with were fabricated for IDD treatment. In vitro studies demonstrated that had ability...
Piezoelectric micromachined ultrasound transducers (PMUTs) have gained significant popularity in the field of ranging and medical imaging owing to their small size, low power consumption, affordability. The scar-free “MIS” (micro-hole inter-etch sealing) process, a novel bulk-silicon manufacturing technique, has been successfully developed for fabrication pressure sensors, flow accelerometers. In this study, we utilize MIS process fabricate cavity diaphragm structures PMUTs, resulting...
The most frequently mutated protein in human cancer is p53, a transcription factor (TF) that regulates myriad genes instrumental diverse cellular outcomes including growth arrest and cell death. Cell context-dependent p53 modulation critical for this life-or-death balance, yet remains incompletely understood. Here we identify sequence signatures enriched genomic p53-binding sites modulated by the cofactor iASPP. Moreover, our p53–iASPP crystal structure reveals iASPP displaces L1 loop—which...