A5044808093- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Microbial Metabolic Engineering and Bioproduction
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Vaccine Coverage and Hesitancy
- Protein purification and stability
- Colorectal Cancer Treatments and Studies
- 3D Printing in Biomedical Research
- Chronic Lymphocytic Leukemia Research
- Biosimilars and Bioanalytical Methods
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 detection and testing
- Lymphoma Diagnosis and Treatment
- Cervical Cancer and HPV Research
- CAR-T cell therapy research
- Hemophilia Treatment and Research
- Lung Cancer Treatments and Mutations
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- Pharmaceutical studies and practices
- Platelet Disorders and Treatments
Sinocelltech Group (China)
2021-2025
Sino Biological (China)
2011-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2025
First Affiliated Hospital of Bengbu Medical College
2025
307th Hospital of Chinese People’s Liberation Army
2016
Massachusetts Institute of Technology
1994-2006
United States Military Academy
2000-2003
The development of human papillomavirus (HPV) vaccines has made substantive progress, as represented by the approval five prophylactic since 2006. Generally, deployment HPV is effective in preventing newly acquired infections and incidences HPV-related malignancies. However, there still a long way to go regarding prevention all eradication established infections, well subsequent progression cancer. Optimizing incorporating L1 proteins from more subtypes, exploring adjuvants that reinforce...
Abstract In animal cell cultivation, density and product concentration are often low due to the accumulation of toxic end‐products such as ammonia lactate and/or depletion essential nutrients. A hybridoma line (CRL‐1606) was cultivated in T‐flasks using a newly devised medium feeding strategy. The goals were decrease formation by design an initial which would provide starting environment achieve optimal growth. This followed stoichiometric equation governing growth then designing...
Lactate and ammonia are the two major waste products formed during mammalian cell growth. Accumulation of these side can have a negative effect on growth, has drawn recent attention because their inhibitory effects specific product synthesis rate. Our aim is to reduce lactate formation in culture by genetically manipulating pathway with an achieve high monoclonal antibody production. We partially disrupted LDH-A gene homologous recombination hybridoma cells (ATCC-CRL-1606). The that received...
Abstract The safety and immunogenicity of a protein-based tetravalent vaccine SCTV01E that contains spike protein ectodomain (S-ECD) Alpha, Beta, Delta Omicron BA.1 are assessed compared with bivalent SCTV01C (Alpha Beta variants) monovalent mRNA (NCT05323461). primary endpoints the geometric mean titers (GMT) live virus neutralizing antibodies (nAb) to (B.1.617.2) at day 28 post-injection. secondary include safety, 180 GMTs against BA.1, BA.5, seroresponse rates T cell responses 450...
COVID-19 vaccines that offer broad-spectrum protection are needed. We aimed to evaluate the safety and immunogenicity of multivalent vaccines, SCTV01E SCTV01C, compare them with an inactivated vaccine.
4092 Background: Hepatocellular carcinoma (HCC) is a significant health concern in China, and it has been leading cause of cancer-related deaths. Immunotherapy combined with anti-vascular growth therapy the first recommended therapy. In this study, we evaluated safety efficacy SCT-I10A (an anti-programmed death 1 [PD-1] monoclonal antibody) SCT510 (a bevacizumab biosimilar) compared to sorafenib as first-line treatment for advanced HCC. Methods: open-label, multicenter, phase 3 trial...
A simple feeding strategy was developed and successfully employed for nutritional control in a 2-L fed-batch culture of hybridoma cells. previously stoichiometric model animal cell growth used to design supplemental medium feeding. Undialyzed fetal bovine serum trace metals (Fe(2+), SeO(3) (2-), Li(+), Zn(2+), Cu(2+)) were fed the cells periodically addition automatic other nutrients medium. In this study, maximum viable density increased from 6.3 x 10(6) 1.7 10(7) cells/mL, span extended...
Animal cell cultivation in vitro has been studied for more than 40 years. However, the culture medium composition not designed on basis of stoichiometric nutritional demands animal growth. In this article, a model was developed to study nutrients (including glucose, 20 amino acids, and 10 vitamins)for synthesis mass product. The coefficients these equation governing growth were determined based composition. addition, detailed analysis nutrients' roles product also performed. Applications...
With the emergence and rapid spread of new pandemic variants, especially variants concern (VOCs), development next-generation vaccines with broad-spectrum neutralizing activities is great importance. In this study, SCTV01C, a clinical stage bivalent vaccine based on trimeric spike extracellular domain (S-ECD) SARS-CoV-2 Alpha (B.1.1.7) Beta (B.1.351) squalene-based oil-in-water adjuvant was evaluated in comparison to its two corresponding (Alpha Beta) monovalent mouse immunogenicity studies....
Background: SCTV01E is a tetravalent recombinant COVID-19 vaccine authorized for emergency use in China adults 18 years and older but not those under 18. Objective: This Phase 2 trial assessed the safety immunogenicity of healthy children adolescents aged 3 to 17 years, establish immunobridging with that observed from efficacy pivotal (NCT05308576). Methods: Participants were randomly assigned receive either 30 µg or placebo. Primary endpoints focused on geometric mean titer (GMT)...
The development of bivalent or multivalent vaccines offers a promising strategy for combating SARS-CoV-2 mutations. In this phase 2 trial, conducted from 1 December 2021, to 25 July 2023, 392 unvaccinated adults aged ≥18 years were randomized receive primary series two doses and booster dose SCTV01C, protein vaccine. Geometric mean titers (GMTs) neutralizing antibodies (nAb) against live Alpha, Beta, Delta, Omicron showed 85.4-, 100.0-, 32.1-, 9.8-fold increase baseline on 28 days, 49.4-,...
SCTV01E, a novel SARS-CoV-2 tetravalent protein vaccine containing recombinant spike proteins of Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1.617.2) and Omicron BA.1 (B.1.1.529.1) variants SCTVA02B adjuvant, has received Emergency Use Authorization (EUA) in China the United Arab Emirates (UAE) as next-generation COVID-19 vaccine. A comprehensive reproductive developmental toxicity evaluation was conducted Sprague-Dawley (SD) rats under Good Laboratory Practice (GLP) conditions. Maternal...
Background/Objectives: Finotonlimab (SCTI10A) is a humanized anti-PD-1 antibody tested in Phase III trials for several solid tumor types. Methods: This study characterized the vitro and vivo efficacy, Fc-mediated effector function, non-clinical PK/PD properties of finotonlimab. Results: The results demonstrated that finotonlimab effective stimulating human T cell function exhibits marked antitumor efficacy using both PD-1-humanized PBMC-reconstructed mouse models. Additionally, exhibited...
Background: Hospitalized patients with severe COVID-19 are at high risk of clinical deterioration. Methods: A global, randomized, double-blinded, and placebo-controlled phase II trial that investigated the efficacy SCTA01, an Fc-modified monoclonal antibody, in hospitalized during Delta variant wave was performed. The primary outcome time to improvement up Day 29. Secondary outcomes measured all-cause mortality rate 29, SARS-CoV-2 RNA negativity number antibody-dependent enhancements....
A detailed reaction network of mammalian cell metabolism contains hundreds enzymatic reactions. By grouping serial reactions into single overall and separating overlapped pathways independent reactions, the total number is significantly reduced. This strategy manipulating avoids manipulations a large otherwise needed to determine extents. stoichiometric material balance model developed based on stoichiometry simplified network. Closures balances glucose each 20 amino acids are achieved using...
Abstract Despite the record speed of developing vaccines and therapeutics against SARS-CoV-2 virus, it is not a given that such success can be secured in future pandemics. In addition, COVID-19 vaccination application remain low countries. Rapid cost mass production antiviral IgY antibodies could an attractive alternative or complementary option for vaccine therapeutic development. this article, we rapidly produced antigens, immunized hens purified 2 months after gene sequence became public....