A5085997877- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Peptidase Inhibition and Analysis
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Hippo pathway signaling and YAP/TAZ
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Synthesis and Catalytic Reactions
- Chemical Synthesis and Reactions
- RNA Research and Splicing
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Molecular Sensors and Ion Detection
- Microbial Natural Products and Biosynthesis
- Trace Elements in Health
- Porphyrin and Phthalocyanine Chemistry
- PI3K/AKT/mTOR signaling in cancer
- Synthesis and Biological Evaluation
- Sulfur-Based Synthesis Techniques
Anhui University
2018-2025
Hefei Institutes of Physical Science
2020-2024
Hefei University
2019-2020
Humboldt-Universität zu Berlin
2016-2018
Tsinghua University
2010-2015
Center for Life Sciences
2012-2015
High Magnetic Field Laboratory
2012-2013
Chinese Academy of Sciences
2012-2013
University of Science and Technology of China
2013
pH determines selectivity: The ligation of peptide hydrazides is a new method for protein chemical synthesis that complementary to native ligation. Peptide may be the long-sought reagent equivalent "thioester synthon", one stable conditions Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note: publisher responsible content functionality any...
Coming together: A generally applicable strategy for convergent chemical synthesis of proteins from multiple peptide segments is developed on the basis ligation hydrazides. The hydrazide intermediates can be made at low cost and new used in 142 residue model protein RpS25 six segments. PG=protecting group.
Fully unprotected peptide o-aminoanilides can be efficiently activated by NaNO2 in aqueous solution to furnish thioesters for use native chemical ligation. This finding enables the convergent synthesis of proteins from readily synthesizable as a new type crypto-thioesters. The practicality this approach is shown histone H2B five segments. Purification or solubilization tags, which are sometimes needed improve efficiency protein synthesis, incorporated into o-aminoanilide moiety, demonstrated...
Der pH-Wert bestimmt die Selektivität: Die Ligation von Peptidhydraziden ergänzt als neues Verfahren der chemischen Proteinsynthese native chemische Ligation. Peptidhydrazid-Reagentien könnten sich lange gesuchtes Äquivalent zu dem "Thioester-Synthon" erweisen, das unter den Bedingungen nativen beständig ist. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted...
Abstract Protein chemical synthesis offers useful and otherwise-difficulty-to-obtain biomacromolecules for biological pharmaceutical studies. Recently, the hydrazide chemistry has drawn attentions in this field as peptide or protein hydrazides can be used key intermediates different modification purposes. Besides being a traditional bioorthogonal handle, group serve readily accessible precursor of thioester. This strategy significantly improves efficiency scope native ligation synthesis....
Rasche Vereinigung: Eine allgemein anwendbare Strategie für die konvergente chemische Synthese von Proteinen aus Peptidsegmenten wurde entwickelt. Die Peptidhydrazid-Zwischenstufen können kostengünstig hergestellt werden, und neue zur des 142 Aminosäuren langen Modellproteins RpS25 sechs eingesetzt. PG=Schutzgruppe. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available...
We report here an enzymatic strategy for asparaginyl endopeptidase-mediated peptide cyclization. Incorporation of chloroacetyl groups into the recognition sequence OaAEP1 enabled intramolecular cyclization with Cys residues. Combining this and phage display, we identified nanomolar macrocyclic ligands targeting TEAD4. One bicyclic peptides binds to TEAD4 a KD value 139 nM, 16 times lower than its linear analogue, demonstrating utility platform in discovering high-affinity ligands.
We report an enzymatic cyclization strategy, termed omniligase-1 mediated peptide bicyclization. Electrophilic group was introduced into the recognition sequence of omniligase to achieve intramolecular bicyclization with Cys residues. Combined phage display, we identified a bicyclic ligand targeting TEAD4 KD value 1.5 µM, 100-fold lower than its linear version, demonstrating utility this platform for discovering ligands.
The striatin-interacting phosphatase and kinase (STRIPAK) complexes integrate extracellular stimuli that result in intracellular activities. Previously, we discovered STRIPAK is a key machinery responsible for loss of the Hippo tumor suppressor signal cancer. Here, identified Hippo-STRIPAK complex as an essential player control DNA double-stranded break (DSB) repair genomic stability. Specifically, found mammalian STE20-like protein kinases 1 2 (MST1/2), independent classical signaling,...
We report the first introduction of an ether linkage as surrogate into disulfide-rich peptides using ether-containing diaminodiacid.
We describe a simple and robust oxidation strategy for preparing N-terminal thiazolidine-containing peptide thioesters from hydrazides. find the first time that l-thioproline can be used as protective agent to prevent nitrosation of thiazolidine during hydrazide oxidation. The thioproline-based has been successfully applied chemical synthesis CC chemokine ligand-2 (69aa) omniligase-C (113aa), thereby demonstrating its utility in hydrazide-based native ligation.
Three states in one blow: a FRET relay system distinguishes between (i) the absence of RNA target, (ii) presence C → U edited or (iii) unedited target by means ratio measurements.
Simple phenyl esters of peptides were found to undergo native chemical ligation with cysteine smoothly under the promotion imidazole. This new proceeded rapidly at both sterically unhindered and hindered C-terminal sites (e.g., Val, Ile, Pro) form desired product in good excellent yields. Detailed facts importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note:...
A new strategy was developed for the synthesis of peptide disulfide-bond mimics using fully orthogonally protected diaminodiacids. This method overcomes previous problems heavy-metal contamination and poor compatibility with Fmoc chemistry provides a practical avenue efficient preparation mimics.
Abstract Fully unprotected peptide o ‐aminoanilides can be efficiently activated by NaNO 2 in aqueous solution to furnish thioesters for use native chemical ligation. This finding enables the convergent synthesis of proteins from readily synthesizable as a new type crypto‐thioesters. The practicality this approach is shown histone H2B five segments. Purification or solubilization tags, which are sometimes needed improve efficiency protein synthesis, incorporated into ‐aminoanilide moiety,...
Abstract Fluorogenic oligonucleotide probes allow mRNA imaging in living cells. A key challenge is the cellular delivery of probes. Most agents, such as cell‐penetrating peptides (CPPs) and pore‐forming proteins, require interactions with membrane. Charges play an important role. To explore influence charge on fluorogenic properties efficiency, we compared peptide nucleic acid (PNA)‐ DNA‐based forced intercalation (FIT) Perhaps counterintuitively, fluorescence signaling by charged DNA FIT...
We report here a robust and practical strategy for chemical protein synthesis using an o-nitrobenzyl group as temporary protective N-terminal cysteine residue of intermediate hydrazide fragments. By reinvestigating the photoremoval group, we establish reliable its quantitative photodeprotection. The is completely stable to oxidative NaNO2 treatment has been applied convergent programmed death ligand 1 fragment, providing avenue hydrazide-based native ligation.