A5012386241- Pain Mechanisms and Treatments
- Nerve injury and regeneration
- RNA and protein synthesis mechanisms
- Spine and Intervertebral Disc Pathology
- Cancer Treatment and Pharmacology
- Neuroscience and Neuropharmacology Research
- RNA regulation and disease
- Molecular Biology Techniques and Applications
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- RNA Research and Splicing
- Receptor Mechanisms and Signaling
- Crystallography and molecular interactions
- Gastrointestinal motility and disorders
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Tissue Engineering and Regenerative Medicine
- Parkinson's Disease Mechanisms and Treatments
- Force Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Ferrocene Chemistry and Applications
- CRISPR and Genetic Engineering
- Nicotinic Acetylcholine Receptors Study
Medizinische Hochschule Hannover
2020-2024
Technische Universität Braunschweig
2015-2021
Gut microbiota is responsible for essential functions in human health. Several communication axes between gut and other organs via neural, endocrine, immune pathways have been described, perturbation of composition has implicated the onset progression an emerging number diseases. Here, we analyzed peripheral nerves, dorsal root ganglia (DRG), skeletal muscles neonatal young adult mice with following status: a) germ-free (GF), b) gnotobiotic, selectively colonized 12 specific bacterial...
Three states in one blow: a FRET relay system distinguishes between (i) the absence of RNA target, (ii) presence C → U edited or (iii) unedited target by means ratio measurements.
A cobaltoceniumethynyl gold(i) complex with a triphenylphosphane ligand triggered efficient cytotoxic effects in cancer cells contrast to derivative two cobaltocenium moieties. The effectively inhibited the enzyme thioredoxin reductase (TrxR) suggesting this as possible biological target. cellular uptake of both metal fragments active was studied by atomic absorption spectroscopy and indicated high stability complex.
C-to-U RNA editing of glycine receptors (GlyR) can play an important role in disease progression temporal lobe epilepsy (TLE) as it may contribute a neuron type-specific way to neuropsychiatric symptoms the disease. It is therefore necessary develop tools that allow identification types express RNA-edited GlyR protein. In this study, we identify NH4 agonist edited GlyRs. Furthermore, generated new molecular sensor tool detects Apobec-1- dependent HEPG2 cells and rat primary hippocampal...
Adult human Schwann cells represent a relevant tool for studying peripheral neuropathies and developing regenerative therapies to treat nerve damage. Primary adult are, however, difficult obtain challenging propagate in culture. One potential solution is generate from induced pluripotent stem (hiPSCs). Previously published protocols, our hands did not deliver sufficient viable cell numbers of hiPSC-derived (hiPSC-SCs). We present here, two modified protocols collaborating laboratories that...
The combined use of “biological” RNA imaging methods based on MS2 technology and “chemical” detection by FIT probes allows unambiguous cellular a C → U edit in mRNA encoding for GlyR α2.
In the current study we investigated suitability of a novel hyaluronic acid-laminin hydrogel (HAL) as luminal filler and carrier system for co-transplanted cells within composite chitosan-based nerve graft (CNG) in rat critical defect model. The HAL was meant to improve performance our artificial guides by giving additional structural molecular support regrowing axons. We filled hollow CNGs or two-chambered with an inserted longitudinal chitosan film (CNG[F]s), cell-free HA additionally...
Hollow nerve guidance conduits are approved for clinical use defect lengths of up to 3 cm. This is because also in pre-clinical evaluation they less effective the support regeneration over critical lengths. Hydrogel luminal fillers thought improve outcome by providing an optimized matrix inside bioartificial grafts. We evaluated here a modified hyaluronic acid-laminin-hydrogel (M-HAL) as filler two clinically hollow guides. Collagen-based and chitosan-based guides were filled with M-HAL...
Glycine receptors (GlyRs) are ligand-gated pentameric chloride channels in the central nervous system. GlyR-α3 is a possible target for chronic pain treatment and temporal lobe epilepsy. Alternative splicing into K or L variants determines subcellular fate function of GlyR-α3, yet it remains to be shown whether its different splice can functionally co-assemble, what properties such heteropentamers would be. Here, we subjected combined fluorescence microscopy electrophysiology analysis. We...
Multiple system atrophy of the parkinsonian type (MSA-P) is a rare, fatal neurodegenerative disease with sporadic onset. It still unknown if MSA-P primary oligodendropathy or caused by neuronal pathophysiology leading to severe, α-synuclein-associated neurodegeneration, mainly in striatum. In this study, we generated and differentiated induced pluripotent stem cells (iPSCs) from patients clinical diagnosis probable (n = 3) three matched healthy controls into GABAergic striatal medium spiny...
Abstract Glycine receptors (GlyRs) are ligand-gated pentameric chloride channels in the central nervous system. GlyR-α3 is a possible target for chronic pain treatment and temporal lobe epilepsy. Alternative splicing into K or L variants determines subcellular fate function of GlyR-α3, yet it remains to be shown whether its different splice can functionally co-assemble, what properties such heteropentamers would be. Here, we subjected combined fluorescence microscopy electrophysiology...