A5083071594- Hippo pathway signaling and YAP/TAZ
- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- Immune cells in cancer
- Electrospun Nanofibers in Biomedical Applications
- Immune Cell Function and Interaction
- Plant Molecular Biology Research
- Plant Genetic and Mutation Studies
- Immunotherapy and Immune Responses
- Plant Pathogenic Bacteria Studies
- Electrochemical sensors and biosensors
- Berberine and alkaloids research
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and biosynthesis
- Cancer Immunotherapy and Biomarkers
- Legume Nitrogen Fixing Symbiosis
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Phagocytosis and Immune Regulation
- Curcumin's Biomedical Applications
- PI3K/AKT/mTOR signaling in cancer
- Aluminum toxicity and tolerance in plants and animals
- Peanut Plant Research Studies
- Fuel Cells and Related Materials
- Plant Disease Resistance and Genetics
Fudan University
2020-2024
Zhongshan Hospital
2021-2024
Shanghai Tenth People's Hospital
2020-2023
Shanghai Institutes for Biological Sciences
2021-2023
Tongji University
2020-2023
University of Chinese Academy of Sciences
2020-2023
Center for Excellence in Molecular Cell Science
2020-2023
Zaozhuang University
2018-2023
State Key Laboratory of Genetic Engineering
2021
Nanjing Agricultural University
2015-2019
Induced resistance response is a potent and cost effective plant defense against pathogen attack. The effectiveness underlying mechanisms of the suppressive ability by Bacillus cereus AR156 to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) in Arabidopsis has been investigated previously; however, strength induced systemic (ISR) activity Botrytis cinerea remains unknown. Here, we show that root-drench application significantly reduces disease incidence through activation ISR. This...
•CD10+ALPL+ neutrophils contribute to tumor resistance anti-PD-1 treatment in patients with HCC.•CD10+ALPL+ exhibit immunosuppressive features the microenvironment.•CD10+ALPL+ trigger irreversible T-cell exhaustion anti-PD-1-resistant patients.•Hepatoma cell-derived NAMPT-NTRK1 axis reprograms CD10+ALPL+ maintain an immature and state. Background & AimsRemodeling microenvironment is a critical strategy for treating advanced hepatocellular carcinoma (HCC). Yet, how distinct cell populations...
Most primary liver cancer (PLC) cases progress mainly due to underlying chronic inflammation, yet the mechanisms of inflammation-mediated PLC remain unclear. Here we uncover a TNF receptor II (TNFR2)-hnRNPK-YAP signaling axis in hepatic progenitor cells (HPC) essential for development. TNFR2, but not I (TNFR1), was required TNFα-induced activation YAP during malignant transformation HPCs and tumorigenesis. Mechanistically, heterogeneous nuclear ribonuclear protein K (hnRNPK) acted downstream...
Hyperactivation of YAP has been commonly associated with tumorigenesis, and emerging evidence hints at multilayered Hippo-independent regulations YAP. In this study, we identified a new MST4-YAP axis, which acts as noncanonical Hippo signaling pathway that limits stress-induced activation. MST4 kinase directly phosphorylated Thr83 to block its binding importin α, therefore leading cytoplasmic retention inactivation. Due consequential interplay between MST4-mediated phospho-Thr83 the...
Abstract As an important part of tumor microenvironment, neutrophils are poorly understood due to their spatiotemporal heterogeneity in tumorigenesis. Here we defined, at single-cell resolution, CD44 −CXCR2 − as tumor-specific (tsNeus) both mouse and human gastric cancer (GC). We uncovered a Hippo regulon with unique YAP signature genes (e.g., ICAM1, CD14, EGR1) distinct from those identified epithelial and/or cells. Importantly, knockout YAP/TAZ neutrophiles impaired differentiation into...
The striatin-interacting phosphatase and kinase (STRIPAK) complexes integrate extracellular stimuli that result in intracellular activities. Previously, we discovered STRIPAK is a key machinery responsible for loss of the Hippo tumor suppressor signal cancer. Here, identified Hippo-STRIPAK complex as an essential player control DNA double-stranded break (DSB) repair genomic stability. Specifically, found mammalian STE20-like protein kinases 1 2 (MST1/2), independent classical signaling,...
Small RNAs function to regulate plant defense responses pathogens. We previously showed that miR825 and miR825* downregulate Bacillus cereus AR156 (AR156)-triggered systemic resistance Pseudomonassyringae pv. tomato DC3000 in Arabidopsis thaliana (Arabidopsis). Here, Northern blotting revealed were more strongly downregulated wild type Col-0 (Col-0) plants pretreated with than nontreated upon Botrytis cinerea (B. cinerea) B1301 infection. Furthermore, compared Col-0, transgenic attenuated...
The Yes-associated protein (YAP) is a major oncoprotein responsible for cell proliferation control. YAP's oncogenic activity regulated by both the Hippo kinase cascade and uniquely mechanical-force-induced actin remodeling process. Inspired reports that ovarian cancer cells specifically accumulate phosphatase ALPP on lipid rafts physically link to cytoskeleton, we developed molecular self-assembly (MSA) technology selectively halts inactivating YAP. We designed ruthenium-complex-peptide...
Low temperatures significantly affect the growth and yield of peanuts. Temperatures lower than 12 °C are generally detrimental for germination To date, there has been no report on precise information quantitative trait loci (QTL) cold tolerance during in In this study, we developed a recombinant inbred line (RIL) population comprising 807 RILs by tolerant sensitive parents. Phenotypic frequencies rate low-temperature conditions among RIL showed normally distributed five environments. Then,...
Hepatocellular carcinoma (HCC) is a fatal malignancy that has limited treatment options. This study focused on the potential therapeutic effects of curcumin (CUR) and berberine (BBR) miR-221/SRY-box transcription factor 11 (SOX11) axis in HCC. We investigated combined CUR BBR HEPG2 Huh7 cell survival miR-221 expression using Cell Counting Kit-8 assays RT-qPCR, respectively. Western blotting was used to detect changes apoptosis-related caspase-3/9 protein levels. performed bioinformatics...
Summary Zoospore chemotaxis to soybean isoflavones is essential in the early stages of infection by oomycete pathogen P hytophthora sojae . Previously, we have identified a G ‐protein α subunit encoded PsGPA1 which regulates and pathogenicity In present study, used affinity purification identify ‐interacting proteins, including s H int1, histidine triad ( HIT ) domain‐containing protein orthologous human nucleotide‐binding 1 HINT1 ). int1 interacted with both guanosine triphosphate GTP )‐...
Abstract Hepatocellular carcinoma (HCC) formation is a multi‐step pathological process that involves evolution of heterogeneous immunosuppressive tumor microenvironment. However, the specific cell populations involved and their origins contribution to HCC development remain largely unknown. Here, comprehensive single‐cell transcriptome sequencing was applied profile rat models toxin‐induced liver tumorigenesis patients. Specifically, we identified three hepatic parenchymal cells emerging...
Ascorbate peroxidases (APXs) maintain cellular reactive oxygen species (ROS) homeostasis through their peroxidase activity. Here, we report that OsAPX1 also promotes ROS production such a delicate is achieved temporally after Magnaporthe oryzae infection. specifically induces increasing respiratory burst oxidase homologs ( OsRBOHs ) expression and can be inhibited by DPI, inhibitor. The time-course experiment data show the simultaneous induction of leads to accumulation at an early stage;...
Blast disease is one of the major rice diseases, and causes nearly 30% annual yield loss worldwide. Resistance genes that have been cloned, however, are effective only against specific strains. In cultivation practice, broad-spectrum resistance to various strains highly valuable, requires researchers investigate basal defense responses for diverse types pathogens. this study, we took a quantitative proteomic approach identified 634 proteins responsive infections by both Magnaporthe oryzae...
Abstract Background: Small RNAs function to regulate plant defense responses pathogens. We previously showed that miR825 and miR825* downregulate Bacillus cereus AR156 (AR156)-triggered systemic resistance Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana (Arabidopsis). The aim of this study was unravel the role AR156-mediated Botrytis cinerea B1301 Arabidopsis. Results: Northern blotting revealed were more strongly downregulated wild type Col-0 (Col-0) plants pretreated with...
Abstract A novel, simple, and rapid method to fabricate thermoresponsive micropatterned substrate for cell adhesion, growth, thermally induced detachment was developed. Thermoresponsive polymer, poly( N ‐isopropylacrylamide) (PNIPAAm), grafted onto the surface of polystyrene (PS) film with microstructure by plasma‐induced graft polymerization technique. The films were characterized attenuated total reflectance‐Fourier transform infrared (ATR‐FTIR) spectroscopy, hydrogen nuclear magnetic...
Rice ARGONAUTE2 (OsAGO2) is a core component of the rice RNA-induced silencing complex (RISC), which repressed by Magnaporthe oryzae (M. oryzae) infection. Whether and how OsAGO2-mediated gene plays role in blast resistance sRNAs participate this process are unknown. Our results indicate that OsAGO2 key immune player manipulates defense responses against disease. associates with 24-nt miR1875 binds to promoter region HEXOKINASE1 (OsHXK1), causes DNA methylation leads silencing. multiple...
Abstract A series of temperature‐sensitive poly(CSA‐ co ‐NIPAAm) membranes that were suitable for cell culture and confluent sheets detachment prepared. The with thermo‐responsive surface properties synthesized by the copolymerization acrylic acid‐derivatized chitosan (CSA) N‐isopropylacrylamide (NIPAAm) in aqueous solution. Characterization carried out means Fourier transform infrared (FTIR), X‐ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM), water contact‐angle...
As coenzyme utilized by more than hundreds of dehydrogenases, the efficient immobilization and regeneration nicotinamide adenine dinucleotide (NAD+) are great importance have practical applications in industrial, analytical biomedical field. In this paper, an electroactive macromolecular weight derivative (PEI-DHBNAD) was prepared attaching both NAD+ 3,4-dihydroxybenzaldehyde (3,4-DHB) to a water-soluble polyelectrolyte, poly(ethylenimine) (PEI). The functional polymer exhibited...
<div>Abstract<p>Most primary liver cancer (PLC) cases progress mainly due to underlying chronic inflammation, yet the mechanisms of inflammation-mediated PLC remain unclear. Here we uncover a TNF receptor II (TNFR2)–hnRNPK–YAP signaling axis in hepatic progenitor cells (HPC) essential for development. TNFR2, but not I (TNFR1), was required TNFα-induced activation YAP during malignant transformation HPCs and tumorigenesis. Mechanistically, heterogeneous nuclear ribonuclear protein...