A5101585672- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Meat and Animal Product Quality
- Circular RNAs in diseases
- Plant tissue culture and regeneration
- Glycosylation and Glycoproteins Research
- Biochemical and Structural Characterization
- Hearing, Cochlea, Tinnitus, Genetics
- Advanced Proteomics Techniques and Applications
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- RNA regulation and disease
- interferon and immune responses
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Helminth infection and control
- Plant Physiology and Cultivation Studies
- Mass Spectrometry Techniques and Applications
Nanjing University of Information Science and Technology
2025
National Animal Husbandry Service
2024-2025
Inner Mongolia Agricultural University
2022-2025
Anhui University
2021-2024
Hefei Institutes of Physical Science
2023-2024
Northeast Forestry University
2011-2024
Henan University of Science and Technology
2024
Sichuan University
2022-2023
Affiliated Zhongshan Hospital of Dalian University
2017-2022
Xiangya Hospital Central South University
2017-2022
Proteome analysis is most commonly accomplished by a combination of two-dimensional gel electrophoresis (2DE) to separate and visualize proteins mass spectrometry (MS) for protein identification. Although this technique powerful, mature, sensitive, questions remain concerning its ability characterize all the elements proteome. In current study, more than 1,500 features were visualized silver staining narrow pH range (4.9–5.7) 2D in which 0.5 mg total soluble yeast was separated. Fifty spots...
BACKGROUND Sensitive and specific biomarkers for the early detection of esophageal squamous cell carcinoma (ESCC) are urgently needed to reduce high morbidity mortality disease. The discovery serum microRNAs (miRNAs) their unique concentration profiles in patients with various diseases makes them attractive, novel noninvasive tumor diagnosis. In this study, we investigated miRNA profile ESCC develop a diagnostic biomarker. METHODS Serum samples were taken from 290 140 age- sex-matched...
Colorectal cancer (CRC) appears to be rather refractory checkpoint blockers except the patient with deficient in mismatch repair (dMMR). Therefore, new advances treatment of most proficiency (pMMR) (also known as microsatellite stability, MSS) type CRC patients are considered an important clinical issue associated programmed death 1 (PD-1) inhibitors. In present study, we evaluated effects gut microbiome MSS-type tumor-bearing mice treated different antibiotics on PD-1 antibody immunotherapy...
Background and Aim Circulating microRNAs (miRNAs) are potential biomarkers for cancer detection; however, little is known about their prognostic impact on oesophageal squamous cell carcinoma (ESCC). The current study aims to uncover novel miRNAs in ESCC patients. Patients Methods We initially screened the expression of 754 serum using TaqMan Low Density Array two pooled samples respectively from 28 normal controls. Markedly upregulated some reported be differently expressed tissue were then...
Circular RNAs (circRNAs) are a class of endogenous noncoding that have been demonstrated to be potential regulators in the development and progression various types human cancer. However, little is known about their roles cancer initiation progression, particular non‑small cell lung (NSCLC). In present study, expression level circRNA‑forkhead box O3 (FOXO3) NSCLC specimens was determined its functional role investigated cells. By performing Taq‑man based RT‑qPCR, it identified circRNA‑FOXO3...
Duddingtonia flagrans is a nematode-trapping fungus that widely used to control parasitic nematodes in livestock. After oral ingestion and passage through the digestive tract of animals, this microorganism captures feces. Although many researchers have examined safety for humans, environment, few reports discussed D. biologics animals. In study, was tested, while adverse effects toxicities were sheep. First, nematode killing naturally parasitized sheep after administration lyophilized...
Exposure to air pollution, especially fine particulate matter (PM2.5), is closely linked various adverse health effects, particularly in the respiratory system. The present study was designed investigate lncRNA–mRNA interactions PM2.5-induced lung cell injury using weighted gene co-expression network analysis (WGCNA). We downloaded expression data of GSE138870 from Gene Expression Omnibus (GEO) database and screened for differentially expressed lncRNAs mRNAs. constructed modules with WGCNA....
Highlights•A multiethnic, multicentric, case–control study was conducted to identify serum miRNA signature for diagnosing NSCLC.•A five-miRNA-based classifier accurate diagnosis of NSCLC among patients Chinese and America constructed.•The five-miRNA panel allowed detection especially stage I–II cases from normal benign nodule subjects.Wang et al. constructed a which provides the potential study. More importantly, subjects. These results suggest that might be useful biomarker in ethnically...
Abstract Long recognized as a threat to wildlife, particularly for large carnivores, livestock grazing in protected areas can potentially undermine conservation objectives. The interspecific interactions among livestock, snow leopards ( Panthera uncia ), and their wild prey fragile Asian highland ecosystems have been subject of debate. We strategically deployed 164 camera traps the Wolong National Nature Reserve systematically investigate activities leopards, primary ungulate species,...
We report here an enzymatic strategy for asparaginyl endopeptidase-mediated peptide cyclization. Incorporation of chloroacetyl groups into the recognition sequence OaAEP1 enabled intramolecular cyclization with Cys residues. Combining this and phage display, we identified nanomolar macrocyclic ligands targeting TEAD4. One bicyclic peptides binds to TEAD4 a KD value 139 nM, 16 times lower than its linear analogue, demonstrating utility platform in discovering high-affinity ligands.
Regulators of G protein signaling (RGS proteins) are well known to accelerate GTPase activity <i>in vitro</i> and promote desensitization vivo</i>. Less is about how RGS proteins themselves regulated. To address this question we purified the in yeast, Sst2, used electrospray ionization mass spectrometry identify post-translational modifications. This analysis revealed that Sst2 phosphorylated at Ser-539 phosphorylation occurs response pheromone stimulation. lies within a consensus...
Lilium pumilum is a perennial herb with ornamental edible and medicinal value. It an excellent wild germplasm resource wide distribution strong resistance. The NAC family of transcription factors unique to higher plants. plays regulatory role in plant growth development participates responses biotic abiotic stresses. LpNAC17 gene L. was cloned transformed into tobacco investigate the response transgenic salt stress. results showed that net photosynthetic rate contents chlorophyll...
We describe a simple and robust oxidation strategy for preparing N-terminal thiazolidine-containing peptide thioesters from hydrazides. find the first time that l-thioproline can be used as protective agent to prevent nitrosation of thiazolidine during hydrazide oxidation. The thioproline-based has been successfully applied chemical synthesis CC chemokine ligand-2 (69aa) omniligase-C (113aa), thereby demonstrating its utility in hydrazide-based native ligation.
We aimed to verify the hypothesis that metabolic syndrome (MetS) and its components affect prognosis of idiopathic sudden sensorineural hearing loss (ISNNHL).A retrospective cohort study.Tertiary otology referral center.We divided patients who were diagnosed with ISSNHL between January 2015 2018 into a MetS group Non-MetS according diagnostic criteria MetS.We by using pure-tone audiometry treated them oral steroids, blood flow promoting agents, hyperbaric oxygen therapy.We used multivariate...
Non-small cell lung cancer (NSCLC) is the most common cause of cancer-associated mortality worldwide. Our previous study revealed that circular RNA (circRNA)-FOXO3 highly expressed in and inhibits proliferation. However, to best our knowledge, at present, no has focused on specific mechanism circRNA-FOXO3 drug resistance. Therefore, present aimed provide novel perspectives role cisplatin (DDP) resistance NSCLC. A Cell Counting Kit-8 assay was used determine viability cells overexpressed with...
An increasing amount of evidence demonstrates that epithelial-mesenchymal transition (EMT) is important in tumor invasion and metastases. The cell-cell adhesion molecule N-cadherin the Wnt/β-catenin cascade protein β-catenin are two biomarkers EMT. present study aimed to measure expression levels samples from patients with nasopharyngeal carcinoma (NPC) evaluate their prognostic significance. mRNA was evaluated using reverse transcription-quantitative polymerase chain reaction 26 NPC tissue...
It has been reported that lncRNA DSCAM-AS1 plays an oncogenic role in breast cancer. In the present study we explored of colorectal adenocarcinoma (CRA).Methods: Gene expression was analyzed by qPCR and Western blot. Overexpression experiments were performed to analyze gene interactions. Transwell assays cell invasion migration. Methylation-specific PCR (MSP) DNA methylation.It observed upregulated primary tumor tissues than paired non-tumor (within 2 cm around tumors) further increased with...
Objectives Recent reports have identified autoimmune systemic diseases as a significant risk factor for sudden sensorineural hearing loss (SSNHL). We investigated whether lupus erythematosus (SLE) and rheumatoid arthritis (RA) were correlated with the recovery of SSNHL. Study Design A retrospective study. Methods Records 663 SSNHL patients between January 2008 2019 retrospectively reviewed, including demographic, comorbid diseases, recovery. Patients divided into four groups (complete,...
The objective of this study was to investigate the effects sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells (SMSCs).Primary SMSCs were isolated from hind leg biceps femoris muscles Wurank lambs (slaughtered at three months, Mth-3) and adults fifteen Mth-15). selected by morphological observation fluorescence staining. Myogenic regulatory factors (MRF) myosin heavy chain (MyHC) expressions analyzed days 1, 3, 4, 5.The factor 5 (Myf5), differentiation (MyoD),...
The identification of proteins differentially expressed between cancer and normal cells is vital for the development diagnostics, therapeutics vaccines. Using a ProteinChip Biomarker System (Ciphergen Biosystems, Fremont, CA) which combines technology with time-of-flight mass spectrometry, we have developed simple method to screen identify secreted from tumor cell lines. Mass spectra range B-cells were generated along those Epstein-Barr virus transformed B-cells. A peak at m/z = 4972.1 that...