Abstract Therapeutic antibodies targeting PD-1 have made major breakthroughs in cancer treatment. However, the majority of colorectal (CRC) cases are microsatellite stable (MSS) and do not respond to anti-PD-1-based immunotherapy. Combination therapy will be an ideal strategy overcome this limitation. Gegen Qinlian decoction (GQD), a classical traditional Chinese medicine (TCM) formula, has been clinically proven effective treatment ulcerative colitis (UC) type 2 diabetes mellitus. Here,...

Colorectal cancer (CRC) appears to be rather refractory checkpoint blockers except the patient with deficient in mismatch repair (dMMR). Therefore, new advances treatment of most proficiency (pMMR) (also known as microsatellite stability, MSS) type CRC patients are considered an important clinical issue associated programmed death 1 (PD-1) inhibitors. In present study, we evaluated effects gut microbiome MSS-type tumor-bearing mice treated different antibiotics on PD-1 antibody immunotherapy...

This study aims to investigate the role of fast-track surgery in preventing development postoperative delirium and other complications elderly patients with colorectal carcinoma. A total 240 carcinoma (aged ≥70 years) undergoing open was randomly assigned into two groups, which were managed perioperatively either traditional or approaches. The length hospital stay (LOS) time pass flatus compared. incidence evaluated. Serum interleukin-6 (IL-6) levels determined before after surgery. LOS...

Abstract Stress ulceration is a common complication in critically ill patients and can result significant upper gastrointestinal bleeding associated with high morbidity mortality. At present, little known of the molecular mechanisms underlying incidence this type gastric damage. In present study, we investigated temporal activation redox-sensitive p38 signaling transduction cascade its roles well-defined experimental model cold immobilization stress-induced ulceration. Exposure...

Ischemia-reperfusion (I/R) injury of the kidney is a complex pathophysiological process and major cause acute renal failure. It has been shown that I/R related to inflammatory responses activation apoptotic pathways. Inhibition certain elements pathway seemed ameliorate injury. As an effective element Panax notoginseng, NR1 antioxidant, anti-inflammatory, antiapoptotic, immune-stimulatory activities. Therefore, we speculate can attenuate was induced by pedicle ligation followed reperfusion...

BACKGROUND We previously showed, using the Traditional Chinese Medicine System Pharmacology Database, that Gegen Qinlian decoction (GQD) had a direct antitumor effect, and was combined with programmed cell death protein (PD)-1 inhibitors to treat microsatellite stable (MSS) tumor-bearing mice. However, effect of GQD on patients colorectal cancer (CRC) is not clear. AIM To determine therapeutic mechanism in improving immune function, reducing inflammation protecting intestinal barrier...

This study aimed to explore the effect of gut microbiota-regulated Kupffer cells (KCs) on colorectal cancer (CRC) liver metastasis.A series in vivo and vitro researches were showed demonstrate microbiota its possible mechanism CRC metastasis.Fewer metastases identified ampicillin-streptomycin-colistin colistin groups. Increased proportions Parabacteroides goldsteinii, Bacteroides vulgatus, thetaiotaomicron, uniformis observed group. The significant expansion KCs was B. vulgatus levels...

Esophageal squamous cell carcinoma (ESCC) is a prevalent and aggressive malignancy, often diagnosed at an advanced stage with poor prognosis. This case report highlights the successful treatment of patient ESCC, who presented bilateral lung adrenal gland metastases. Despite nature disease, underwent regimen first-line chemotherapy combined immunotherapy, followed by immune maintenance therapy. Remarkably, achieved complete remission after completion treatment, demonstrating potential...

Cancer patients are particularly vulnerable to the psychological sequels of COVID-19 due their immunocompromised state and disruptions regular oncological care. There is limited research comparing effects SARS-CoV-2 on cancer co-living individuals. This study aims explore similarities differences in physical outcomes between these two groups, with a 1-year follow-up assess long-term effects. retrospective observational was conducted January February 2023. A total 107 participants were...

Programmed cell death receptor 1 (PD-1) and T immunoglobulin mucin-3 (Tim-3) are considered as important immunosuppressive molecules play an role in tumor immune escape cancer progression. However, it remains unclear whether PD-1 Tim-3 coexpressed stage I-III colorectal (CRC) how they impact on the prognosis of disease.A total two cohorts with 451 patients who underwent surgery for CRC treatment were enrolled study. Among which, 378 cases from The Cancer Genome Atlas (TCGA) database 73...

To investigate the effects of recombinant human endostatin Endostar on metastasis and angiogenesis lymphangiogenesis colorectal cancer cells in a mouse xenograft model. Colon SW620 were injected subcutaneously into left hind flank nude mice to establish models. The treated with normal saline or every other day. growth lymph node tumor cells, tissue detected. Apoptosis cell cycle distribution studied by flow cytometry. expression VEGF-A, -C, -D was determined immunoblotting assays. inhibited...

Platelet-activating factor acetylhydrolase IB subunit beta (PAFAH1B2) plays important roles in inflammation and anaphylaxis. However, its primary function pancreatic cancer remains unclear. In the current study, we report that PAFAH1B2 is overexpressed ductal adenocarcinoma (PDAC) correlated inversely with patient survival. overexpression induced epithelial–mesenchymal transition (EMT), migration invasion vitro metastasis vivo. Conversely, silencing inhibited these aggressive phenotypes....

BRAFV600E mutated colorectal cancer (CRC) is prone to peritoneal and distant lymph node metastasis this correlates with a poor prognosis. The mutation closely related the formation of an immunosuppressive microenvironment. However, correlation between changes in local immune microenvironment CRC not clear.To explore effect mechanism mutant on CRC.Thirty patients were included study: 20 control group 10 treatment group. density microvessels microlymphatic vessels, M2 subtype macrophages tumor...

Stress ulceration is a common complication in critically ill patients, but the mechanisms involved are poorly understood. In this study we investigated temporal activation of redox-sensitive transcription factor nuclear factor-kappaB and its roles an experimental model cold immobilization stress-induced gastric mucosal lesions.Prospective, controlled, randomized animal study.University research laboratory.Male Sprague-Dawley rats.The rats were subjected to stress for total 6 hrs. The...

The present study evaluated the distribution of sympathetic and parasympathetic nerves expression α9 nicotinic acetylcholine receptor (α9nAChR) investigated their potential association with colorectal cancer (CRC) development. autonomic α9nAChR in CRC was detected by immunohistochemistry, which then used to analyze clinicopathological parameters prognosis. Sympathetic fibers were primarily observed stroma adjacent cells, whereas away from cells. Patients samples positive for nerve had less...

To investigate the expression of vascular endothelial growth factor-C (VEGF-C) and relationship between VEGF-C lymphangiogenesis, lymph node metastasis in colorectal cancer.Fifty six cases cancer were selected randomly. Expression was detected by immunohistochemistry, lymphatic vessels stained enzyme histochemical method.VEGF-C found 66.7% (37/56) patients. In positive negative patients, vessel density 25.16+/-7.52 17.14+/-7.22, respectively (P<0.05). The rate patients (81.1%) significantly...

The present study was designed to find out whether SB431542, an inhibitor of transforming growth factor beta1 activin receptor-like kinase, could protect the lung from LPS-induced injury. Inflammatory injury model induced by intratracheal administration LPS. C57BL/6 mice were randomly divided into sham control group (S group), LPS stimulation (L + early SB431542 treatment (Ie and delayed (Id group). admitted intraperitoneally on days 1, 2, 3 in Ie group, whereas those Id received same dose...

The aim of the present study was to examine role protease-activated receptor-1 (PAR1)-stimulated platelet activation in epithelial-mesenchymal transition (EMT) and migration colon cancer cells, identify underlying mechanisms. TFLLR-NH2, a PAR1 agonist, used activate platelets supernatants were treat SW620 cell line. Expression E-cadherin vimentin on cells detected by immunofluorescence western blotting, level transforming growth factor β1 (TGF-β1) measured using ELISA following TFLLR-NH2....

The aim of the present study was to investigate effect hepatocyte growth factor receptor (c-MET) inhibition on viability colon cancer cells and xenografts exposed irradiation using short hairpin (sh)RNA or c-MET inhibitor PHA665752. underlying mechanisms were also investigated. Human colorectal adenocarcinoma HT-29 infected with a lentivirus expressing shRNAs against irradiated at 0, 2, 4, 6 8 Gy. assessed by alamarBlue® assays. Mice bearing human carcinoma SW620 randomly selected receive...

Colorectal cancer (CRC) remains one of the most common types worldwide. A few previous studies have examined whether HER4 may promote progression CRC. The present study associations among expression levels members HER family, and investigated potential mechanism underlying function in CRC cells. Immunohistochemistry analysis was conducted to detect family patients with knocked down using short hairpin RNA HCT116 cells, confirmed by quantitative PCR western blotting. proliferation adhesion...