A5083486771- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Biocrusts and Microbial Ecology
Northeastern Illinois University
2014-2018
A bacteriophytochrome from Stigmatella aurantiaca is an unusual member of the family that devoid hydrogen bonding to carbonyl group ring D biliverdin (BV) chromophore. The photodynamics BV in SaBphP1 wild type and single mutant T289H reintroducing show strength this particular weak interaction determines excited-state lifetime, Lumi-R quantum yield, spectral heterogeneity. In particular, decay faster absence hydrogen-bonding D, with half-lives 30 80 ps for mutant, respectively....
Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr Pfr distinct spectra the red far-red. They have recently been engineered enzymatic photoswitches for fluorescent-marker applications non-invasive tissue imaging mammals. This article presents cryo- room-temperature crystal structures...
The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation lacking. Using solution X-ray scattering, we find that the photosensory cores several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. data establish with ordinary inverted photocycles share signaling mechanism particular conserved histidine,...