A5012662201- Advanced Fluorescence Microscopy Techniques
- Digital Holography and Microscopy
- Biocrusts and Microbial Ecology
- Neuroscience and Neuropharmacology Research
- Crystallization and Solubility Studies
- Light effects on plants
- X-ray Diffraction in Crystallography
- Photosynthetic Processes and Mechanisms
- Cell Image Analysis Techniques
- Ion channel regulation and function
- Optical Coherence Tomography Applications
- Cardiac electrophysiology and arrhythmias
- Chemical Reactions and Isotopes
- Robotic Path Planning Algorithms
- Alzheimer's disease research and treatments
- Magnetism in coordination complexes
- Modular Robots and Swarm Intelligence
- Neuroinflammation and Neurodegeneration Mechanisms
- Protein Tyrosine Phosphatases
- Photoacoustic and Ultrasonic Imaging
- ATP Synthase and ATPases Research
- Advanced Biosensing Techniques and Applications
- Lanthanide and Transition Metal Complexes
- Robotic Locomotion and Control
- Metal complexes synthesis and properties
University of Illinois System
2024
University of Illinois Urbana-Champaign
2021-2023
Northeastern Illinois University
2017-2019
University of Gothenburg
2019
University of Wisconsin–Milwaukee
2019
Japan Synchrotron Radiation Research Institute
2019
SPring-8
2019
University of Chicago
2019
Kyoto University
2019
University of Toledo
2015
Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr Pfr distinct spectra the red far-red. They have recently been engineered enzymatic photoswitches for fluorescent-marker applications non-invasive tissue imaging mammals. This article presents cryo- room-temperature crystal structures...
Primary neuronal cultures have been widely used to study morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning memory. However, the unique behavioral properties neurons make them challenging study, with phenotypic differences expressed as subtle changes in arborization rather than easy-to-assay features such cell count. The need analyze growth, intracellular transport has motivated development increasingly sophisticated...
Nanoparticles have emerged as potential transporters of drugs targeting Alzheimer's disease (AD), but their design should consider the blood-brain barrier (BBB) integrity and neuroinflammation AD brain. This study presents that aging is a significant factor for brain localization retention nanoparticles, which we engineered to bind with reactive astrocytes activated microglia. We assembled 200 nm-diameter particles using block copolymer poly(lactic-co-glycolic acid) (PLGA) CD44-binding...
Phytochromes (PHYs) are photoreceptor proteins first discovered in plants, where they control a variety of photomorphogenesis events. PHYs as photochromic can reversibly switch between two distinct states: red light (Pr) and far-red (Pfr) absorbing form. The discovery Bacteriophytochromes (BphPs) nonphotosynthetic bacteria has opened new frontiers our understanding the mechanisms by which these natural photoswitches single cell development, although role BphPs vivo remains largely unknown....
Synthesis, characterization, cyclic voltammetry, magnetic susceptibility and crystal structures of mono dinuclear macrocyclic cobalt(<sc>ii</sc>) complexes have been investigated.
Abstract Objective STriatal‐Enriched protein tyrosine Phosphatase (STEP) is a brain‐specific phosphatase. Membrane‐bound STEP 61 the only isoform expressed in hippocampus and cortex. Genetic deletion of enhances excitatory synaptic currents long‐term potentiation hippocampus. However, whether affects seizure susceptibility unclear. Here we investigated effects inhibitor TC‐2153 on propensity murine model displaying kainic acid (KA)–induced status epilepticus its effect hippocampal...
Mobile manipulators and humanoid robots should have the ability to use objects in their environments. Previous work has shown significant advantages that can remove interfere with goal. We propose next step. Just like fictional character 'MacGyver,' construct simple machines tools from arbitrary objects. This video presents our progress developing validating 'MacGyver' skills a simulated rescue mission.
Neuronal K v 7/Potassium Voltage-Gated Channel Subfamily Q (KCNQ) potassium channels underlie M-current that potently suppresses repetitive and burst firing of action potentials (APs). They are mostly heterotetramers 7.2 7.3 subunits in the hippocampus cortex, brain regions important for cognition behavior. Underscoring their critical roles inhibiting neuronal excitability, autosomal dominantly inherited mutations Potassium Member 2 ( KCNQ2 ) 3 KCNQ3 genes associated with benign familial...
Homeostatic plasticity encompasses the mechanisms by which neurons stabilize their synaptic strength and excitability in response to prolonged destabilizing changes network activity. Prolonged activity blockade leads homeostatic scaling of action potential (AP) firing rate hippocampal part decreased N-Methyl-D-Aspartate receptors subsequent transcriptional down-regulation potassium channel genes including KCNQ3 encodes K v 7.3. Neuronal 7 channels are mostly heterotetramers 7.2 7.3 subunits...
We present artificial confocal microscopy (ACM) to achieve confocal-level depth sectioning, sensitivity, and chemical specificity non-destructively on unlabeled specimens. ACM is equipped with a laser scanning quantitative phase imaging module, which provides optical path-length maps of the specimen colocalized fluorescence channel. Using pairs images, convolution neural network was trained translate former into latter. The images hold much stronger sectioning than input (phase) enabling us...
Primary neuronal cultures have been widely used to study morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning memory. Yet, the unique behavioral properties neurons make them challenging - with phenotypic differences expressed as subtle changes in arborization rather than easy assay features such cell count. The need analyze growth, intracellular transport has motivated development increasingly sophisticated microscopes...
Bacteriophytochromes (BphPs) are red‐light photoreceptor proteins found in photosynthetic and non‐photosynthetic bacteria. They unique among photoreceptors their ability to undergo reversible photoconversion between spectrally distinct red (Pr, 700nm) far‐red (Pfr, 750nm) light absorbing states. BphPs composed of an N‐terminal photosensory module, consisting PAS, GAF, PHY domains, covalently linked effector domain, usually a histidine kinase. For photoresponse, require biliverdin (BV),...
Widefield microscopy methods applied to optically thick specimens are faced with reduced contrast due spatial crosstalk, in which the signal at each point is result of a superposition from neighboring points that simultaneously illuminated. In 1955, Marvin Minsky proposed confocal as solution this problem. Today, laser scanning fluorescence broadly used its high depth resolution and sensitivity, come price photobleaching, chemical, photo-toxicity. Here, we present artificial (ACM) achieve...