A5009449260- Digital Holography and Microscopy
- Advanced Fluorescence Microscopy Techniques
- Optical measurement and interference techniques
- Advanced X-ray Imaging Techniques
- Cell Image Analysis Techniques
- Optical Coherence Tomography Applications
- Kruppel-like factors research
- Epigenetics and DNA Methylation
- Photoacoustic and Ultrasonic Imaging
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Immune cells in cancer
- Microfluidic and Bio-sensing Technologies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- Natural Compounds in Disease Treatment
- Photoreceptor and optogenetics research
- Advanced Measurement and Metrology Techniques
- Random lasers and scattering media
- Glutathione Transferases and Polymorphisms
- Micro and Nano Robotics
- Nuclear Structure and Function
Washington University in St. Louis
2020-2024
Applied Materials (United States)
2024
University of Illinois Urbana-Champaign
1973-2023
University of Science and Technology Beijing
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2011-2022
Duke University
2018
Tianjin University
2015
Cancer Institute (WIA)
2014
State Key Laboratory of Molecular Oncology
2014
Molecular Oncology (United States)
2014
Abstract Existing approaches to evaluate cell viability involve staining with chemical reagents. However, the step of exogenous makes these methods undesirable for rapid, nondestructive, and long-term investigation. Here, we present an instantaneous assessment unlabeled cells using phase imaging computation specificity. This concept utilizes deep learning techniques compute markers associated specimen measured by label-free quantitative imaging. Demonstrated on different live cultures,...
Macrophages play a crucial role in tumorigenesis depending upon the phenotype of macrophages found tumor microenvironments. To date, how microenvironment affects phenotypes is not yet fully understood. In this study, we constructed NIH3T3/Src cell line stably overexpresses Src protein and that conditioned medium from was able to induce polarization towards M2 primary bone marrow-derived (BMDM) Ana-1 macrophages. Further investigation revealed IL-6 produced by cells plays key polarization....
Abstract Multiple scattering and absorption limit the depth at which biological tissues can be imaged with light. In thick unlabeled specimens, multiple randomizes phase of field attenuates light that travels long optical paths. These obstacles performance transmission imaging. To mitigate these challenges, we developed an epi-illumination gradient interference microscope (epi-GLIM) as a label-free imaging modality applicable to bulk or opaque samples. Epi-GLIM enables studying turbid...
Traditional methods for cell cycle stage classification rely heavily on fluorescence microscopy to monitor nuclear dynamics. These inevitably face the typical phototoxicity and photobleaching limitations of imaging. Here, we present a detection workflow using principle phase imaging with computational specificity (PICS). The proposed method uses neural networks extract cycle-dependent features from quantitative (QPI) measurements directly. Our results indicate that this approach attains very...
Abstract Emerging evidence has shown the association of aberrantly expressed miR‐106a with cancer development, however, little is known about its potential role in gastric carcinogenesis. In our present study, obviously overexpressed was found tissues compared their nontumor counterparts. Suppression significantly inhibited cell proliferation and triggered apoptosis. Bioinformatic analysis combining validation experiments identified FAS as a direct target miR‐106a. Rescue examination...
Quantitative phase imaging (QPI) is an emerging label-free modality that attracts significant interest in biomedicine general and neuroscience particular. Based on the principle of interferometry, QPI precisely maps optical pathlength induced by sample, and, thus, can visualize extremely transparent samples. The field has grown rapidly past decade, reliable instruments have been developed for in-depth biological studies. One particular figure merit associated with techniques describes...
In 1969, Emil Wolf proposed diffraction tomography using coherent holographic imaging to extract 3D information from transparent, inhomogeneous objects. the same era, equations were first used describe propagation correlations associated with partially fields. Combining these two concepts, we present phase (WPT), which is a method for performing WPT reconstruction works directly in space-time domain, without need Fourier transformation, and decouples refractive index (RI) distribution...
Oesophageal squamous cell carcinoma (ESCC) occurs at a very high rates in certain regions of China. There are increasing evidences demonstrating that selenium could act as potential anti-oesophageal cancer agent, but the precise mechanisms involved still not completely understood. Methylseleninic acid (MSA), potent second-generation compound, is promising chemopreventive agent. Previous studies demonstrated kelch-like ECH-associated protein 1 (Keap1)/nuclear factor E2-related 2 (Nrf2) system...
Esophageal squamous cell carcinoma (ESCC) is a leading cause of cancer-related death in China and has limited effective therapeutic options except for early surgery, since the underlying molecular mechanism driving its precursor lesions towards invasive ESCC not fully understood. Cellular senescence state permanent growth arrest cell, considered as initial barrier tumor development. Human differentiated embryo chondrocyte expressed gene 1 (Dec1) an important transcription factor that related...
Esophageal squamous cell carcinoma (ESCC) occurs at a very high frequency in certain areas of China. Supplementation with selenium‐containing compounds was associated significantly lower cancer mortality rate study conducted Linxia, Thus, selenium could be potential anti‐esophageal agent. In this study, methylseleninic acid (MSA) inhibit growth ESCC cells vitro and vivo. Upon treated MSA, the activity histone deacetylases (HDACs) decreased general control nonrepressed protein 5 (GCN5)...
Krüppel-like factor 4 (KLF4) is a transcription and functions as tumor suppressor or promoter in different cancer types. KLF4 regulates many gene expression, thus affects the process of cell proliferation, differentiation, apoptosis. Recently, was reported to induce senescence during generation induced pluripotent stem (iPS) cells, but exact mechanism still unclear. In this study, we constructed two doxycycline-inducing models, demonstrated overexpression could promote senescence, detected...
This soft machine can pick up and release an ultrathin “living” cell sheet electronics without crumpling.
Wnt/β-catenin and Hippo pathways play essential roles in the tumorigenesis development of colorectal cancer. We found that Celastrol, isolated from Tripterygium wilfordii plant, exerted a significant inhibitory effect on cancer cell growth vitro vivo, further unraveled molecular mechanisms. Celastrol induced β-catenin degradation through phosphorylation Yes-associated protein (YAP), major downstream effector pathway, also Celastrol-induced was dependent liver kinase B1 (LKB1). increased...
Radiotherapy is one of the main treatments for esophageal squamous cell carcinoma, but there are still no biomarkers to differentiate patients who will benefit from radiation. Although treatment with a combination radiotherapy chemotherapy, and/or surgery improves prognosis patients, can distinguish between responses obtained combined therapies. Therefore, in this study, we selected treated alone evaluate survivin as predictor radiotherapy. One hundred two biopsy samples collected by...
Optogenetics has emerged as an exciting tool for manipulating neural activity, which in turn, can modulate behavior live organisms. However, detecting the response to optical stimulation requires electrophysiology with physical contact or fluorescent imaging at target locations, is often limited by photobleaching and phototoxicity. In this paper, we show that phase report intracellular transport induced optogenetic stimulation. We developed a multimodal instrument both stimulate cells...
In this Letter, we present, to our knowledge, the first endoscopic diffraction phase microscopy (eDPM) system. This instrument consists of a gradient-index-lens-based endoscope probe followed by DPM module, which enables single-shot imaging at single-cell-level resolution. Using information provided eDPM, show that geometric aberrations associated with can be reduced digitally applying spectral filter raw data. The function is linear combination polynomials weighting optimized improve We...
The tumorigenesis and progression of colorectal cancer are closely related to the tumor microenvironment, especially inflammatory response. Inhibitors histone deacetylase (HDAC) have been reported as epigenetic regulators immune system treat diseases our results demonstrated that Celastrol could act a new HDAC inhibitor. Considering macrophages important members we further found influence polarization inhibit cell growth. Specially, used supernatant HCT116 SW480 cells induce Ana-1 in vitro...
The deep learning-based image segmentation approach has evolved into the mainstream of target detection and shape characterization in microscopic analysis. However, accuracy generalizability learning approaches are still hindered by insufficient data problem that results from high expense human material resources for acquisition annotation. Generally, augmentation can increase amount a short time means mathematical simulation, become necessary module In this work, we first review commonly...
Ovarian cancer is the leading cause of death in women worldwide. Cisplatin core first-line chemotherapy for patients with advanced ovarian cancer. Many eventually become resistant to cisplatin, diminishing its therapeutic effect. MicroRNAs (miRNAs) have critical functions diverse biological processes. Using miRNA profiling and polymerase chain reaction validation, we identified a panel differentially expressed miRNAs their potential targets cisplatin-resistant SKOV3/DDP cells relative...
Aurora-A overexpression is common in various types of cancers and has been shown to be involved tumorigenesis through different signaling pathways, yet how the deregulation affects cancer therapeutics remains elusive. Here we showed that rendered esophageal cells resistance cisplatin (CDDP) by inhibiting apoptosis. By using an apoptosis array, identified a downstream gene, p21-activated kinase 7 (PAK7). PAK7 was upregulated at both mRNA protein levels. Importantly, expression levels were...
We studied collagen fiber organization in tissue affected by pelvic organ prolapse (POP) and compared it to asymptomatic controls. Both the control POP biopsies were prepared measured a highly sensitive quantitative phase imaging (QPI) system, called spatial light inference microscopy (SLIM). Combined with automatic image processing, this modality provides quantitative, high-throughput assessment of morphology. found orientation prolapsed specimens is less homogeneous, indicating an abnormal...