A5015818000- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Graphene and Nanomaterials Applications
- Lysosomal Storage Disorders Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Cancer Research and Treatments
- Nanoparticles: synthesis and applications
- Photodynamic Therapy Research Studies
- Acoustic Wave Resonator Technologies
- Nanocluster Synthesis and Applications
- Chemical Synthesis and Analysis
- Extracellular vesicles in disease
- Cellular transport and secretion
- bioluminescence and chemiluminescence research
- Ferroelectric and Piezoelectric Materials
- Plant chemical constituents analysis
- Parasitic Diseases Research and Treatment
- Glycogen Storage Diseases and Myoclonus
- Cancer Cells and Metastasis
- Advanced X-ray Imaging Techniques
- Click Chemistry and Applications
- Microwave Dielectric Ceramics Synthesis
- Biochemical and Molecular Research
National Enterprise for NanoScience and NanoTechnology
2024
Istituto Nanoscienze
2016-2024
IPB University
2024
Scuola Normale Superiore
2016-2023
Center for Nanotechnology Innovation
2016-2020
Italian Institute of Technology
2016-2020
University of Illinois Urbana-Champaign
2019
National University of the Littoral
2010
The transferrin receptor (TfR) is a promising target in cancer therapy owing to its overexpression most solid tumors and on the blood–brain barrier. Nanostructures chemically derivatized with are employed TfR targeting but often lose their functionality upon injection bloodstream. As an alternative strategy, we rationally designed peptide coating able bind suitable pockets not involved binding or iron by using iterative multiscale-modeling approach coupled quantitative structure–activity...
GALC-loaded nanoparticles can reinstate enzymatic activity in the brain of mouse model Krabbe disease.
Here, we report: (i) the straightforward production of narrow-NIR-absorbing gold ultrasmall-in-nano architectures (<italic>t</italic>NAs), and (ii) their suitability as excretable platforms for photothermal therapy upon CW-irradiation at 808 nm. These findings are a significant step toward translation metal nanotheranostics to cancer treatments.
Effective excretion of nanostructured noble metals is still one the most challenging bottlenecks for their employment in clinical practice. Besides persistence issue, translation inorganic nanomaterials also affected by a bewildering lack investigations regarding quantitative biokinetics. Here, we have quantitatively correlated chemical nature three interesting biomedical applications to biosafety and biokinetics in, respectively, zebrafish murine models. Gold, silver, platinum...
Accumulation of inorganic nanostructures in the excretory system organs increases their likelihood toxicity and interference with common medical diagnoses. Thus, one major concerns regarding clinical translation is related to persistence organisms. Here authors demonstrate that nano-architectures composed by hollow silica nanocapsules embedding arrays ultrasmall gold nanoparticles undergo biodegradation cellular environment affording small, potentially clearable building blocks. Furthermore,...
Polymeric nanoparticles (NPs) represent one of the most promising tools in nanomedicine and have been extensively studied for delivery water-insoluble drugs. However, efficient loading therapeutic enzymes proteins polymer-based nanostructures remains an open challenge. Here, we report a synthesis method new enzyme system based on cross-linked aggregates (CLEAs) encapsulation into poly(lactide- co-glycolide) (PLGA) NPs. We tested strategy four currently investigated replacement therapy:...
After inhalation, ultrasmall-in-nano gold architectures accumulate in the lungs, reach secondary organs, and are excreted, encouraging clinical translation of plasmonic nanomaterials to management cancer infectious diseases.
A protein corona-modulating peptide is integrated with non-persistent plasmonic nano-architectures containing a cisplatin prodrug to improve the efficacy of nanomaterial-mediated chemotherapy in alternative vivo models oral carcinoma.
Stealth agents are extensively investigated as a means by which to prolong nanostructure residence time in the bloodstream avoiding uptake reticuloendothelial system. Unfortunately, commonly used such poly(ethylene glycol) can adversely impact targeting efficiency and promote immune reaction host organism. Therefore, there is an increasing interest developing biocompatible, non-PEGylated organic nanostructures able perform targeted delivery increase efficacy of liposomal technology. Here,...
Several nanomaterials rely on the passive accumulation in neoplasm target because of enhanced permeability and retention effect. On other hand, directing to by employing targeting agents may lead a pivotal improvement efficacy treatment for number cancers. However, moieties often lose their functionality upon injection bloodstream, leaving questions efficiency. Here, we assessed using significant vitro 3D model pancreatic carcinoma efficiency passion fruit-like nanoarchitectures (NAs)...
Negative or positive HPV-associated Head and Neck Squamous Cell Carcinomas (HNSCCs) are high recurrence neoplasms usually resulting in a poor prognosis, mainly due to metastasis formation. Despite the low overall patient survival rate severe side effects, treatment of choice is still cisplatin-based chemotherapy. Here, we report straightforward protocol for production throughput 3D models negative HNSCCs, together with their employment therapeutic evaluation gold ultrasmall-in-nano...
As a first approach, standard 2D cell culture techniques are usually employed for the screening of drugs and nanomaterials. Despite easy handling, findings achieved on cultures often not efficiently translatable to in vivo preclinical investigations. Furthermore, although animal models pivotal studies, more strict directives have been implemented promote use alternative biological systems. In this context, development integration into research workflow 3D neoplasm is particularly appealing...
CLN5 disease (MIM: 256731) represents a rare late-infantile form of neuronal ceroid lipofuscinosis (NCL), caused by mutations in the gene that encodes protein (CLN5p), whose physiological roles stay unanswered. No cure is currently available for patients and opportunities therapies are lagging. The role lysosomes neuro-pathophysiology an important topic since lysosomal proteins directly involved primary mechanisms injury occurring various NCL forms. We developed implemented lysosome-focused,...
Head and neck squamous cell carcinomas (HNSCCs) are a complex group of malignancies that affect different body sites pertaining to the oral cavity, pharynx larynx. Current chemotherapy relies on platinum complexes, major exponent being cisplatin, which exert severe side effects can negatively prognosis. For this reason, other metal complexes with less investigated as alternatives or adjuvants complexes. In context, exploiting (supra)additive by concurrent administration cisplatin emerging is...
Abstract Biological samples are mainly composed of elements with a low atomic number which show relatively electron scattering power. For Transmission Electron Microscopy analysis, biological generally embedded in resins, allow thin sectioning the specimen. Embedding resins also by light atoms, thus contrast difference between sample and surrounding resin is minimal. Due to that reason last decades, several staining solutions approaches, performed heavy metal salts, have been developed...
We studied collagen fiber organization in tissue affected by pelvic organ prolapse (POP) and compared it to asymptomatic controls. Both the control POP biopsies were prepared measured a highly sensitive quantitative phase imaging (QPI) system, called spatial light inference microscopy (SLIM). Combined with automatic image processing, this modality provides quantitative, high-throughput assessment of morphology. found orientation prolapsed specimens is less homogeneous, indicating an abnormal...
Nanoparticles are widely used as theranostic agents for the treatment of various pathologies, including cancer. Among all, dendrimers-based nanoparticles represent a valid approach drugs delivery, thanks to their controllable size and surface properties. Indeed, dendrimers can be easily loaded with different payloads functionalized targeting agents. Moreover, they in combination other materials such metal combinatorial therapies. Here, we present formulation an innovative nanostructured...
Infantile neural ceroid lipofuscinosis (INCL) is a lysosomal storage disorder characterized by mutations in the CLN1 gene that leads to lack of enzyme palmitoyl-protein thioesterase-1 (PPT1), which causes progressive death cortical neurons. Enzyme replacement therapy (ERT) one most promising treatments, but its translation toward clinical use hampered need deliver central nervous system and more detailed understanding capability restore physiologic conditions at biochemical protein level,...
The effective exploitation of the intriguing theranostic features noble metal nanoparticles for therapeutic applications is far from being a routine practice due to persistence issue. In this regard, passion fruit-like nano-architectures (NAs), biodegradable and excretable all-in-one, nature-inspired platforms which jointly combine these characteristics with appealing optical behaviors nanoparticles, can offer new alternative applications. Besides need efficacious innovative systems,...
Granulysin is a human polypeptide produced by cytolytic cells active against broad range of microbes. Three peptides covering the regions 25-50 (Gr-1 and Gr-2) 39-62 (Gr-3) granulysin were synthesized, their in vitro activity Mycobacterium tuberculosis was evaluated. The most peptide Gr-1C, containing disulphide bridge, with Minimal Inhibitory Concentration value 10.1 microM. In concentrations up to 50 microM, Gr-1 Gr2 didn't exceed 30% hemolysis.