A5001519037- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Microbial Community Ecology and Physiology
- Wastewater Treatment and Nitrogen Removal
- Protist diversity and phylogeny
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Genomics and Phylogenetic Studies
- Protein Structure and Dynamics
- Microbial Fuel Cells and Bioremediation
- Plant nutrient uptake and metabolism
- Biochemical and Molecular Research
- Chromium effects and bioremediation
- Photosynthetic Processes and Mechanisms
- Pancreatic function and diabetes
- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Marine and coastal ecosystems
- GABA and Rice Research
- Bacterial Genetics and Biotechnology
- Higher Education Teaching and Evaluation
- Electrochemical sensors and biosensors
- Chemical Analysis and Environmental Impact
- Metalloenzymes and iron-sulfur proteins
- Microbial bioremediation and biosurfactants
University of Alicante
2013-2024
Universidad de Murcia
1987-1989
The structure of glucose dehydrogenase from the extreme halophile Haloferax mediterranei has been solved at 1.6-Å resolution under crystallization conditions which closely mimic “ in vivo ” intracellular environment. decoration enzyme’s surface with acidic residues is only partially neutralized by bound potassium counterions, also appear to play a role substrate binding. shows expected reduction hydrophobic character, surprisingly not changes associated loss exposed but rather arising...
The nitrogen cycle (N-cycle), principally supported by prokaryotes, involves different redox reactions mainly focused on assimilatory purposes or respiratory processes for energy conservation. As the N-cycle has important environmental implications, this biogeochemical become a major research topic during last few years. However, although metabolic pathways have been studied extensively in Bacteria Eukarya, relatively little is known Archaea. Halophilic Archaea are predominant microorganisms...
Despite being the subject of intensive investigations, many aspects mechanism zinc-dependent medium chain alcohol dehydrogenase (MDR) superfamily remain contentious. We have determined high-resolution structures a series binary and ternary complexes glucose dehydrogenase, an MDR enzyme from Haloferax mediterranei. In stark contrast to textbook in which zinc ion is proposed stationary attached common set protein ligands, analysis these reveals that each complex, there are dramatic differences...
Abstract A set of 110 extremely halophilic archaeal strains were isolated from seven distinct saline habitats located in different regions Algeria. The physicochemical characterization the samples showed that these thalassohaline. carotenoid production varied 0.1 to 3.68 µg/ml. Based on their physiological characteristics and pigment production, 43 selected identified by means phenotypic tests 16S ribosomal RNA gene sequencing. Phylogenetic analysis indicated isolates corresponded class...
Haloferax mediterranei, an extreme halophilic archaeon thriving in hypersaline environments, has acquired significant attention biotechnological and biochemical research due to its remarkable ability flourish salinity conditions. Transcription factors, essential regulating diverse cellular processes, have become focal points understanding adaptability. This study delves into the role of Lrp transcription factor, exploring modulation glnA, nasABC, lrp gene promoters vivo through...
Leather biodegradation is a microbially driven process of increasing interest for the development sustainable waste management strategies. In this study, bacterial communities involved in leather tanned with different agents (chrome, zeolite, Biole®) were characterised using high-throughput sequencing. Taxonomic profiling based on 16S rRNA gene amplification revealed that Proteobacteria, Bacteroidetes, and Patescibacteria dominant phyla across samples. Functional analysis, carried out...
An NAD(P)‐glucose dehydrogenase from the extremely halophilic Archaeon, Haloferax mediterranei , has been purified to electrophoretic homogeneity. The enzyme characterised with respect its cofactor specificity, subunit composition and salt thermal stability. N‐terminal amino acid sequence determined N‐terminus alignment sequences of other glucose dehydrogenases shows that most closely resembles NAD(P)‐linked thermophilic Archaeon Thermoplasma acidophilum . However, appears be a dimeric...
The extraction of salt from seawater by means coastal solar salterns is a very well-described process. Moreover, the characterization these environments ecological, biochemical and microbiological perspectives has become key focus for many research groups all over world last 20 years. In countries such as Spain, there are several examples (mainly on Mediterranean coast) inland salterns, which sodium chloride obtained human consumption. However, studies focused scarce both archaeal diversity...
The occurrence of the glyoxylate cycle has not previously been demonstrated in any Archaea. In halophilic archaea, only isocitrate lyase activity detected. archaeon Haloferax volcanii was tested for presence other key enzyme this pathway, malate synthase. High activities were detected when carbon source acetate. Both enzymes, and synthase, from Hf . purified characterized.
The nitrite reductase from the extreme halophilic archaeon, Haloferax mediterranei, has been purified and characterised. H. mediterranei is capable of growing in a minimal medium (inorganic salts glucose as carbon source) with nitrate only nitrogen source. overall purification was 46-fold about 4% recovery activity. enzyme monomeric protein approximately 66 kDa. A pH 7.5 high temperatures up to 60°C are necessary for optimum Reduced methyl viologen found be an electron donor effective...
The first gene encoding a glucose dehydrogenase (GDH) from halophilic organism has been sequenced. Amino acid sequence alignments of GDH Haloferax mediterranei show high degree homology with the thermoacidophilic GDHs and other enzymes medium chain dehydrogenase/reductase family. Heterologous overexpression using mesophilic Escherichia coli as host performed expression product was obtained inclusion bodies. To obtain enzyme in its native form refolding reactivation saline environment were...