A5004877841- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Allergic Rhinitis and Sensitization
- Education, Healthcare and Sociology Research
- Asthma and respiratory diseases
- Microstructure and Mechanical Properties of Steels
- Analytical Chemistry and Chromatography
- Protein Structure and Dynamics
- Fatigue and fracture mechanics
- Metabolomics and Mass Spectrometry Studies
- Immunodeficiency and Autoimmune Disorders
- Metal Alloys Wear and Properties
- Bacterial Genetics and Biotechnology
- Porphyrin Metabolism and Disorders
- Pediatric health and respiratory diseases
- Gynecological conditions and treatments
- Endometriosis Research and Treatment
- RNA and protein synthesis mechanisms
- Research in Social Sciences
- Hydrogen embrittlement and corrosion behaviors in metals
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Endometrial and Cervical Cancer Treatments
- Hemoglobin structure and function
- ATP Synthase and ATPases Research
University of Gothenburg
2014-2024
Science for Life Laboratory
2023-2024
National University of Singapore
2021
Cornell University
2019
Göteborgs Stads
2017
Chalmers University of Technology
2003-2012
Pfizer (Sweden)
2012
Stockholm University
1978-2012
Pharma Nord (United Kingdom)
2011
Octapharma (Austria)
2011
Aluminium alloys AA 5083 and 6082 have been friction stir welded the mechanical properties microstructures of welds evaluated. Alloy mainly fractured near centre weld, while fracture in occurred heat affected zone. The tensile strength joints was lower than base material strength, but still met classification societies’ requirements. Hardness approximately constant across zone 5083, a minimum hardness found welds. location closely matched region. Very fine scale precipitation significantly...
A spectroelectrochemical method has been used to determine the reduction potential of copper site in wild‐type and 22 mutant forms azurin from Pseudomonas aeruginosa at 25 °C range pH 4–8; effect buffers ionic strength on potentials also studied. Amino‐acid residues changed include Met121, which provides an S atom a distance about 0.3 nm metal, some amino acids hydrophobic patch, other believed be important electron transfer with physiological partners internal acids. The observed span 300...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTReorganization Energy of Blue Copper: Effects Temperature and Driving Force on the Rates Electron Transfer in Ruthenium- Osmium-Modified AzurinsAngel J. Di Bilio, Michael G. Hill, Nicklas Bonander, B. Göran Karlsson, Randy M. Villahermosa, Bo Malmström, Jay R. Winkler, Harry GrayView Author Information Beckman Institute, California Institute Technology Pasadena, 91125 Department Biochemistry Biophysics Göteborg University, Medicinaregatan 9C...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRaman Spectroscopy as an Indicator of Cu-S Bond Length in Type 1 and 2 Copper Cysteinate ProteinsColin R. Andrew, Hyeyeong Yeom, Joan Selverstone Valentine, B. Goeran Karlsson, Gertie van Pouderoyen, Gerard W. Canters, Thomas M. Loehr, Joann Sanders-Loehr, N. BonanderCite this: J. Am. Chem. Soc. 1994, 116, 25, 11489–11498Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue December...
The reaction of fully reduced soluble bovine heart cytochrome oxidase with O2 at 173K was investigated by low-temperature optical and e.p.r. spectroscopy, the kinetics were analysed non-linear optimization techniques. only signals seen during course are those attributable to low-spin a3+ CuA2+. Quantitative analysis shows that, end point 173K, nearly 100% CuA is in cupric state but about 40% a ferric state. spectra recorded this stage show incomplete oxidation haem absence 655 nm absorption...
Somatic mutations in the POLE gene encoding catalytic subunit of DNA polymerase ε have been found sporadic colorectal cancers (CRCs) and are most likely importance tumour development and/or progression. Recently, families with dominantly inherited adenomas cancer were shown to a causative heterozygous germline mutation proofreading exonuclease domain POLE. The highly penetrant was associated predisposition CRC only no extra-colonic tumours observed. We identified large family which carriers...
The purpose of this study was to define the cumulative incidence pregnancy rhinitis, and whether smoking, asthma, hayfever or month conception are risk factors. A questionnaire delivered during 1 year in 10 antenatal care centers one Swedish county. Questions were asked by midwives on ordinary check-up visits throughout pregnancy. Five with response rates 70% more, including 599 women, evaluated. rhinitis 22%. Smokers had a significantly increased relative enhancement 69%, whereas hayfever,...
The structural gene for the blue copper protein azurin from Pseudomonas aeruginosa has been subcloned in different expression plasmid vectors. highest yield of was obtained when with its native ribosome-binding site placed downstream lac promoter pUC18. is exported to periplasmic space Escherichia coli and amount corresponds 27% total content space. preprotein cleaved correctly according N-terminal sequencing purified protein. Azurin large amounts spectroscopically indistinguishable P. aeruginosa.
Cassette mutagenesis was used to exchange the suggested copper ligand Met121 in azurin all other amino acids, and a stop codon. The mutant proteins were characterized by optical absorption spectroscopy EPR. At low pH, mutants exhibit characteristics of blue type 1 protein, indicating that methionine is not needed create site. high Glu121 Lys121 constitute new form protein-bound outside range type1 copper.
A new EPR signal from Cu2+ has been discovered in reductive experiments with type 2 copper-depleted laccase Polyporus versicolor. novel also found native Rhus vernicifera on oxidation of the reduced protein H2O2. In reoxidation cytochrome c oxidase beef heart, a observed. With laccase, is shown to originate one copper ions that are nondetectable resting enzyme, and evidence presented for signals stemming metal pairs antiferromagnetically coupled oxidized enzymes. The show strong rhombic...