A5017060034- Enzyme Structure and Function
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Glutathione Transferases and Polymorphisms
- Viral gastroenteritis research and epidemiology
- Protein Structure and Dynamics
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- SARS-CoV-2 and COVID-19 Research
- Genomics, phytochemicals, and oxidative stress
- Advanced X-ray Imaging Techniques
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- Plant Disease Resistance and Genetics
- X-ray Spectroscopy and Fluorescence Analysis
- Plant and Fungal Interactions Research
- Dental Research and COVID-19
- Bacillus and Francisella bacterial research
- Metabolism and Genetic Disorders
- Spaceflight effects on biology
- Aldose Reductase and Taurine
- Polyoxometalates: Synthesis and Applications
- Metal-Organic Frameworks: Synthesis and Applications
- Advanced Chemical Sensor Technologies
- Biochemical and Molecular Research
Hiroshima University
2018-2025
Osaka University
2011-2022
Protein Research Foundation
2008-2019
Institute for Public Relations
2014-2015
University of California, Davis
2010
Karolinska Institutet
2010
Central Region Agricultural Research Center
2010
Max Planck Institute of Biophysics
2008
Abstract Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at Linac Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, aerosolized injected into approximately 0.1 μm diameter focused hard beam CXI LCLS. Diffraction patterns with signal to...
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, small particles has proven to be difficult two reasons: not quite high enough pulse intensity from currently available lasers and, as we demonstrate here, contamination the aerosolized molecules by nonvolatile contaminants in solution. amount sample depends initial droplet size during aerosolization. Here, show that, with our electrospray injector,...
The P9-1 protein of Rice black streaked dwarf virus accumulates in viroplasm inclusions, which are structures that appear to play an important role viral morphogenesis and commonly found viruses the family Reoviridae. Crystallographic analysis revealed structural features allow form dimers via hydrophobic interactions. Each dimer has carboxy-terminal regions, resembling arms, extend neighboring dimers, thereby uniting sets four lateral interactions, yield cylindrical octamers. importance...
Chlorous acid water and sodium hypochlorite solution are effective disinfectants against SARS-CoV-2, the virus that caused COVID-19 pandemic. Recent studies have shown both compounds equivalent inactivation effects when tested on purified viruses. However, in practical applications, presence of organic matter is common can significantly affect disinfectant performance. We conducted several experiments comparing these two under different conditions to better understand their efficacy. When an...
ABSTRACT The conservation of the core structure and diversification external features among turreted reoviruses appear to be relevant structural evolution in facilitating infection diverse host species. Rice ragged stunt virus (RRSV), genus Oryzavirus family Reoviridae , is determined show a composed capsid shell, clamps, long turrets. RRSV equivalent Orthoreovirus virion Cytoplasmic polyhedrosis (CPV). In RRSV, five peripheral trimers surround each turret sit at Q trimer position T=13 l...
Ethanol is an effective disinfectant against the novel coronavirus SARS-CoV-2. However, its concentration has not been shown, and we therefore analyzed effects of different concentrations ethanol on When SARS-CoV-2 was treated with varying examined for changes in infectivity, at which 99% infectious titers were reduced 24.1% (w/w) [29.3% (v/v)]. For reference, susceptibility also other envelope viruses, including influenza virus, vesicular stomatitis virus family Rhabdoviridae, Newcastle...
Abstract The decrease of antibody efficacy to mutated SARS-CoV-2 spike RBD explains the breakthrough infections and reinfections by Omicron variants. Here, we analyzed broadly neutralizing antibodies isolated from long-term hospitalized convalescent patients early strains. One named NCV2SG48 is highly potent broad variants including BA.1, BA.2, BA.4/5. To reveal mode action, determined sequence crystal structure Fab fragment in a complex with original, Delta, BA.1. minor V H but multiple...
The non-structural Pns9 protein of rice gall dwarf virus (RGDV) accumulates in viroplasm inclusions, which are structures that appear to play an important role viral morphogenesis and commonly found host cells infected by viruses the family Reoviridae. Immunofluorescence immunoelectron microscopy RGDV-infected vector monolayers, using antibodies against RGDV expression Spodoptera frugiperda cells, demonstrated is minimal factor necessary for formation inclusion during infection RGDV. When...
Hepatitis E virus (HEV) accounts for the majority of enterically transmitted hepatitis infections worldwide. Currently, there is no specific treatment or vaccine against HEV. The major structural protein derived from open reading frame (ORF) 2 viral genome. A potential oral provided by virus-like particles formed a construct partial ORF3 (residue 70–123) fused to N-terminus ORF2 (residues 112–608). Single crystals obtained hanging-drop vapour-diffusion method at 293 K diffract X-rays 8.3 Å...
Journal Article The functional organization of the internal components Rice dwarf virus Get access Naoyuki Miyazaki, Miyazaki * 1Department Molecular and Cellular Biology, University California, Davis, CA 95616, USA; 2Karolinska Institute Structural Virology, 14157 Huddinge, Sweden; 3National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666; 4Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, 565-0871, Japan *Naoyuki Japan, Tel.: +81 6 6879 8608,...
Previously, we found an unclassified glutathione S-transferase 2 (bmGSTu2) in the silkworm Bombyx mori that conjugates to 1-chloro-2,4-dinitrobenzene and also metabolises diazinon, organophosphate insecticide. Here, provide a structural genome-editing characterisation of diazinon-metabolising B. mori. The structure bmGSTu2 was determined at 1.68 Å by X-ray crystallography. Mutation putative amino acid residues substrate-binding site showed Pro13, Tyr107, Ile118, Phe119, Phe211 are crucial...
We first investigated the interactions between several algae-derived lectins and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). created lectin columns using high-mannose (HM)-type glycan-specific OAA KAA-1 or core fucose-specific hypninA-2 conducted binding experiments with SARS-CoV-2. The results showed that these were capable of to virus. Furthermore, when examining neutralization ability nine different lectins, it was found KAA-1, ESA-2, effective in neutralizing In...
We prepared polyoxomolybdates with methylammonium countercations from monomolybdate, (CH3NH3)2[MoO4], through two dehydrative condensation methods, acidifying in the aqueous solution and solid-state heating. Discrete (CH3NH3)10[Mo36O112(OH)2(H2O)14], polymeric ((CH3NH3)8[Mo36O112(H2O)14])n, ((CH3NH3)4[γ-Mo8O26])n were selectively isolated via pH control of (CH3NH3)2[MoO4] solution. The H2SO4-acidified < 1 produced "sulfonated α-MoO3", ((CH3NH3)2[(MoO3)3(SO4)])n. heating air released...
Journal Article Electron microscopic imaging revealed the flexible filamentous structure of cell attachment protein P2 Rice dwarf virus located around icosahedral 5-fold axes Get access Naoyuki Miyazaki, Miyazaki * 1 Institute for Protein Research, Osaka University, Suita, Osaka, Japan; 2 Supportive Center Brain National Physiological Sciences, Okazaki, Aichi, *Naoyuki 38 Nishigonaka, Myodaiji, Aichi 444-8585, Japan. Tel: +81-56-455-7872 , Fax: +81-56-452-7913 email: naomiya@nips.ac.jp...
Recently, many technical improvements in macromolecular X-ray crystallography have increased the number of structures deposited Protein Data Bank and improved resolution limit protein structures. Almost all high-resolution been determined using a synchrotron radiation source conjunction with cryocooling techniques, which are required order to minimize damage. However, optimization cryoprotectant conditions is time-consuming difficult step. To overcome this problem, high-pressure method was...
An influenza virus epidemic is an important issue in public hygiene, and continuous development on effective drug required. Kampo medicine a traditional that used clinically for treatment of various diseases Japan other East Asian countries. We evaluated the effects drugs maoto, kakkonto, senkyuchachosan, jinkokato, bakumondoto, which are prescribed respiratory symptoms including caused by influenza, replication cultured cells. Culture media virus-infected MDCK(+) cells were tested...
The immune evasion of SARS-CoV-2 Omicron variants caused by multiple amino acid replacements in the receptor-binding domain (RBD) spike protein wanes effectiveness antibodies elicited current booster vaccination. vaccines that target strains have been recently developed, however, there has a concern yet to be addressed regarding negative aspect response known as original antigenic sin. Here, we demonstrate breadth neutralizing against is barely immunizing monovalent viral antigens via...
Beamline BL44XU at SPring-8 is operated by the Institute for Protein Research of Osaka University. The beamline designed X-ray crystallography large biological macromolecular assemblies. Here we show its detailed performances, results, and ongoing upgrade plans.