A5101770519- Ferroelectric and Piezoelectric Materials
- Advanced Fluorescence Microscopy Techniques
- Electronic and Structural Properties of Oxides
- Holomorphic and Operator Theory
- Advanced biosensing and bioanalysis techniques
- Protein Structure and Dynamics
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- ZnO doping and properties
- DNA and Nucleic Acid Chemistry
- Copper-based nanomaterials and applications
- Electron and X-Ray Spectroscopy Techniques
- Algebraic and Geometric Analysis
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Molecular Junctions and Nanostructures
- Advanced Electron Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Microwave Dielectric Ceramics Synthesis
- DNA Repair Mechanisms
- Analytic and geometric function theory
- Cystic Fibrosis Research Advances
- Heat shock proteins research
- Magnetic and transport properties of perovskites and related materials
- RNA and protein synthesis mechanisms
TU Dresden
2015-2025
Université de Bordeaux
2015-2023
Institut de Mathématiques de Bordeaux
2004-2023
Institut Polytechnique de Bordeaux
2017-2023
Centre National de la Recherche Scientifique
2017-2023
Bioengineering Center
2020
Klinik und Poliklinik für Psychotherapie und Psychosomatik
2020
Laboratoire Paul Painlevé
2019
University of Richmond
2019
PNSensor (Germany)
2011-2012
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro vivo. However, generalized protocols FRET standards ensure the reproducibility accuracy measurements efficiencies are currently lacking. Here we report results a comparative blind study which 20 labs determined (E) several dye-labeled DNA duplexes. Using unified, straightforward method, obtained with s.d. between ±0.02 ±0.05. We...
Abstract Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study of biomolecular structure and dynamics in vitro vivo. We performed an international blind involving 19 laboratories to assess uncertainty FRET for proteins with respect measured efficiency histograms, determination distances, detection quantification structural dynamics. Using two protein systems distinct conformational changes dynamics, we obtained ≤0.06, corresponding interdye distance precision...
The alkaline single cell gel test (SCG or comet assay) was used to study the contribution of excision repair activity observed DNA effect mutagen treatment. cytotoxicity and genotoxicity UV-irradiation chemical mutagens 4-nitroquinoline-1-oxide (4NQO), benzo[a]pyrene (BP) 7,12-dimethy-benz[a]anthracenentracene (DMBA) were compared in a normal human line (MRC5CV1) an excision-deficient xeroderma pigment-osum (XP) (XP12ROSV). XP cells showed increased killing after treatment with all tested,...
Abstract Cellular senescence is a program activated during diverse situations of cell stress. Chondrocytes differ from other somatic cells as articular cartilage an avascular tissue. The effects oxidative stress on chondrocytes are still unknown. Our studies were to investigate into the proliferation potential, cytological features and telomere linked response system human osteoarthritic chondrocytes, subjected acute or prolonged oxidant challenge with hydrogen peroxide. Telomere length was...
Single-molecule Förster resonance energy transfer (smFRET) has become a powerful nanoscopic tool in studies of biomolecular structures and nanoscale objects; however, conventional smFRET measurements are generally blind to distances above 10 nm thus impeding the study long-distance phenomena. Here, we report development farFRET, technique that extends range beyond line by enhanced using multiple acceptors. We demonstrate farFRET can be readily employed quantify FRET efficiencies...
The voltage and film composition dependence of leakage currents ferroelectric SrBi2Ta2O9 thin films, sandwiched between Pt has been studied. Schottky emission dominated the current at voltages above ohmic conduction regime, while space charge limited (SCLC), for which observed temperature is correctly predicted in Rose’s theory, appeared to dominate high conductivity films including bismuth-excess samples. A consequence latter was observation negative differential resistivity films. X-ray...
We demonstrate the growth of epitaxial Fe/Ba(Fe(1-x)Co(x))2As2 (Fe/Ba-122) bilayers on MgO(001) and LSAT(001) single crystal substrates using Pulsed Laser Deposition (PLD). By exploiting metallic nature FeAs tetrahedron in Ba-122 structure, we achieve a coherent interfacial bond between bcc iron Co-doped Ba-122. Tc values for both were close to that PLD target. Direct observation bonding Fe sublattice by atomic resolution transmission electron microscopy implies this bilayer architecture may...
Periplasmic chaperones 17-kilodalton protein (Skp) and survival factor A (SurA) are essential players in outer membrane (OMP) biogenesis. They prevent unfolded OMPs from misfolding during their passage through the periplasmic space aid disassembly of OMP aggregates under cellular stress conditions. However, functionally important links between interaction mechanisms, structural dynamics, energetics that underpin both Skp SurA associations with have remained largely unresolved. Here, using...
Abstract Force spectroscopy gives access to the underlying free energy landscape of protein folding. Proteins exhibit folding rates between microseconds and hours. To slow rates, magnetic tweezers have shown be a promising tool, yet it remained unclear if capture kinetics ultra-fast proteins. Here, we study mechanics λ 6-85 ; five-helix bundle with fast ~20 µs time in thermal denaturation midpoint. We observed two-state at ~ 6.2 pN 250 ms mechanical With optical tweezers, found that folds...
Long- and short-range structural order of nanocrystalline ZnO films grown using single source chemical vapor deposition (SS-CVD) basic zinc acetate Zn4O(CH3COO)6 have been investigated. The addition a low background pressure H2O during SS-CVD provides mechanism for varying the properties through control impurities occluded within film. structure resultant can be varied from amorphous (10 at. % impurities) to highly crystalline with preferred c-axis orientation (less than 1 impurities)....
Abstract We have used x-ray photoelectron spectroscopy (XPS) and ultraviolet absorption measurements to determine unambiguous values of the electron affinities band structure alignments for three most popular ferroelectric memory materials (strontium bismuth tantalate SBT. lead zirconate-titanate PZT, barium strontium titanate BST) on platinum electrodes. The PZT/Pt results model disagree quantitatively qualitatively with earlier work Wouters, Willems, Maes [Microelectron. Eng. 29, 249...
Dynamic loading and perfusion culture environments alone are known to enhance cartilage extracellular matrix (ECM) production in dedifferentiated articular chondrocytes. In this study, we explored whether a combination of these factors would processes over free-swelling (FS) condition using adult human chondrocytes embedded 2% alginate. The alginate constructs were placed into bioreactor for (P) only (100 μL/per minute) or dynamic compressive (PL) (20% 1 h, at 0.5 Hz), each day. Control FS...
Single-molecule FRET (smFRET) has become a versatile tool for probing the structure and functional dynamics of biomolecular systems, is extensively used to address questions ranging from folding drug discovery. Confocal smFRET measurements are amongst widely assays typically performed in single-well format. Thus, sampling many experimental parameters laborious time consuming. To this challenge, we extend here capabilities confocal beyond by integrating multiwell plate functionality allow...
Biologically functional DNA hairpins are found in archaea, prokaryotes and eukaryotes, playing essential roles various transactions. However, during replication, hairpin formation can stall the polymerase is therefore prevented by single-stranded binding protein (SSB). Here, we address question how maintain their secondary structure despite SSB's presence. As a model hairpin, used recombinogenic form of attC site, for capturing antibiotic-resistance genes integrons bacteria. We that have...
Changes in molecular conformations are one of the major driving forces complex biological processes. Many studies based on single-molecule techniques have shed light conformational dynamics and contributed to a better understanding living matter. In particular, FRET experiments revealed unprecedented information at various time scales varying from milliseconds seconds. The choice attachment fluorophores is pivotal requirement for experiments. One particularly well-studied millisecond change...
Abstract Our meagre understanding of CFTR misfolding and its reversal by small-molecule correctors hampers the development mechanism-based therapies cystic fibrosis. Here we exploit a helical-hairpin construct—the simplest proxy membrane-protein tertiary contacts—containing CFTR’s transmembrane helices 3 4 corresponding disease phenotypic mutant V232D to gain molecular-level insights into drug rescue corrector Lumacaftor. Using single-molecule FRET approach study hairpin conformations in...
Ribonucleoprotein (RNP) granules have been linked to translation regulation and disease, but their assembly regulatory mechanisms are not well understood. Here, we show that the RNA-binding protein G3BP1 preferentially interacts with unfolded RNA, driving of RNP granule-like condensates establish RNA-RNA interactions. These interactions limit mobility translatability sequestered mRNAs stabilize condensates. The DEAD-box RNA helicase DDX3X attenuates inside condensates, rendering dynamic...
An increasing number of membrane proteins in different membrane-mimetic systems have become accessible to reversible unfolding experiments monitored by well-established ensemble techniques. However, only little information is available about kinetic processes during membrane-protein folding, mainly because experimental challenges and a lack methods suitable for observing highly dynamic proteins. Here, we present single-molecule Förster resonance energy transfer (smFRET) confocal spectroscopy...