A5074578778- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- RNA Research and Splicing
- Protein Structure and Dynamics
- RNA modifications and cancer
- Advanced Fluorescence Microscopy Techniques
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Electron Spin Resonance Studies
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Advanced Biosensing Techniques and Applications
- Genomics and Phylogenetic Studies
- Ecology, Conservation, and Geographical Studies
- Drug Transport and Resistance Mechanisms
- Libraries and Information Services
- Lanthanide and Transition Metal Complexes
- Biocrusts and Microbial Ecology
- Bacteriophages and microbial interactions
- Metal complexes synthesis and properties
- Microbial Natural Products and Biosynthesis
Heinrich Heine University Düsseldorf
2015-2024
GP Forschungsgruppe
2006
St. Josefskrankenhaus Freiburg
2006
University of Würzburg
1987
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro vivo. However, generalized protocols FRET standards ensure the reproducibility accuracy measurements efficiencies are currently lacking. Here we report results a comparative blind study which 20 labs determined (E) several dye-labeled DNA duplexes. Using unified, straightforward method, obtained with s.d. between ±0.02 ±0.05. We...
Abstract FRET experiments can provide state-specific structural information of complex dynamic biomolecular assemblies. However, to overcome the sparsity experiments, they need be combined with computer simulations. We introduce a program suite ( i ) an automated design tool for which determines how many and pairs should used minimize uncertainty maximize accuracy integrative structure, ii efficient approach FRET-assisted coarse-grained modeling, all-atom molecular dynamics simulations-based...
Guanylate binding proteins (GBPs) are soluble dynamin-like that undergo a conformational transition for GTP-controlled oligomerization and disrupt membranes of intracellular parasites to exert their function as part the innate immune system mammalian cells. We apply neutron spin echo, X-ray scattering, fluorescence, EPR spectroscopy techniques integrative dynamic structural biology study basis mechanism transitions in human GBP1 (hGBP1). mapped hGBP1's essential dynamics from nanoseconds...
IHMCIF (github.com/ihmwg/IHMCIF) is a data information framework that supports archiving and disseminating macromolecular structures determined by integrative or hybrid modeling (IHM), making them Findable, Accessible, Interoperable, Reusable (FAIR). an extension of the Protein Data Bank Exchange/macromolecular Crystallographic Information Framework (PDBx/mmCIF) serves as for (PDB) to archive experimentally atomic biological macromolecules their complexes with one another small molecule...
The mechanical heterogeneity of biomolecular structures is intimately linked to their diverse biological functions. Applying rigidity theory biomolecules identifies this heterogeneous composition flexible and rigid regions, which can aid in the understanding stability long‐ranged information transfer through biomolecules, yield valuable for rational drug design protein engineering. We review fundamental concepts theory, ways represent as constraint networks, methodological algorithmic...
Riboswitches are genetic regulatory elements that control gene expression depending on ligand binding. The guanine-sensing riboswitch (Gsw) binds ligands at a three-way junction formed by paired regions P1, P2, and P3. Loops L2 L3 cap the P2 P3 helices form tertiary interactions. Part of P1 belongs to switching sequence dictating fate mRNA. Previous studies revealed an intricate relationship between binding presence interactions, influence region. However, no information is available...
This paper was originally published under standard Springer Nature copyright. As of the date this correction, Analysis is available online as an open-access with a CC-BY license. No other part has been changed.
Riboswitches are genetic regulatory elements mainly found in bacteria, which regulate gene expression based on the availability of a ligand. Purine-sensing riboswitches, including guanine-sensing riboswitch (Gsw), possess tertiary interactions connecting L2 and L3 loops. These important for ligand binding to aptamer. However, atomic-level structural knowledge about unbound state how influence conformational heterogeneity aptamer is still scarce. We performed replica exchange molecular...