A5084592241- Protein Structure and Dynamics
- Advanced Fluorescence Microscopy Techniques
- Hippo pathway signaling and YAP/TAZ
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Electrocatalysts for Energy Conversion
- Signaling Pathways in Disease
- Enzyme Structure and Function
- Gestational Diabetes Research and Management
- Microtubule and mitosis dynamics
- Cancer-related gene regulation
- Metalloenzymes and iron-sulfur proteins
- Endoplasmic Reticulum Stress and Disease
- Hydrogen Storage and Materials
- Pregnancy and preeclampsia studies
- Heme Oxygenase-1 and Carbon Monoxide
- Supramolecular Self-Assembly in Materials
- Antimicrobial Peptides and Activities
- 14-3-3 protein interactions
- Optical Imaging and Spectroscopy Techniques
- Diabetes Management and Research
- Cellular Mechanics and Interactions
- Molecular Junctions and Nanostructures
- Plant biochemistry and biosynthesis
- Advanced Biosensing Techniques and Applications
Tufts University
2024-2025
City College of New York
2022-2024
Stony Brook University
2010-2018
University of California, San Diego
2017
Institute of Pharmacology
2012
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro vivo. However, generalized protocols FRET standards ensure the reproducibility accuracy measurements efficiencies are currently lacking. Here we report results a comparative blind study which 20 labs determined (E) several dye-labeled DNA duplexes. Using unified, straightforward method, obtained with s.d. between ±0.02 ±0.05. We...
Quantum defects in single-walled carbon nanotubes promote exciton localization, which enables potential applications biodevices and quantum light sources. However, the effects of local electric fields on emissive energy states how they can be controlled are unexplored. Here, we investigate defect sensitization by engineering an intrinsically disordered protein to undergo a phase change at site. We designed supercharged single-chain antibody fragment (scFv) enable full ligand-induced folding...
Scaffold proteins form a framework to organize signal transduction by binding multiple partners within signaling pathway. This shapes the output of responses as well providing specificity and localization. The Membrane Associated Guanylate Kinases (MAGuKs) are scaffold at cellular junctions that localize cell surface receptors link them downstream enzymes. often contain protein-binding domains connected in series disordered linkers. tertiary structure folded is understood, but describing...
Ancestral metabolic processes involve the reversible oxidation of molecular hydrogen by hydrogenase. Extant hydrogenase enzymes are complex, comprising hundreds amino acids and multiple cofactors. We designed a 13–amino acid nickel-binding peptide capable robustly producing from protons under wide variety conditions. The forms di-nickel cluster structurally analogous to Ni-Fe in [NiFe] Ni-Ni acetyl-CoA synthase, two ancient, extant proteins central metabolism. These experimental results...
Otitis media (OM) is the most common infectious disease of children under six, causing more antibiotic prescriptions and surgical procedures than any other pediatric condition. By screening a bacteriophage (phage) library genetically engineered to express random peptides on their surfaces, we discovered unique that actively transport phage particles across intact tympanic membrane (TM) into middle ear (ME). Herein our goals were characterize physiochemical peptide features may underlie...
This paper was originally published under standard Springer Nature copyright. As of the date this correction, Analysis is available online as an open-access with a CC-BY license. No other part has been changed.
The VX nerve agent is one of the deadliest chemical warfare agents. Specific, sensitive, real-time detection methods for this neurotoxin have not been reported. creation proteins that use biological recognition to fulfill these requirements using directed evolution or library screening has hampered because its toxicity makes laboratory experimentation extraordinarily expensive. A pair VX-binding were designed a supercharged scaffold couples large-scale phase change from unstructured folded...
Many proteins are modular in that they contain a series of independently‐folded domains. Often, these sub‐domains have no affinity for each other solution, but can maintain stable supertertiary structure when connected by polypeptide linkers. Little is known about the ultraweak interactions dictate stability structure. It our hypothesis surface features select specific structure, while linker length determines effective concentration thereby setting interaction energy. To test this we...