A5100778515- Hepatitis B Virus Studies
- Genomics and Chromatin Dynamics
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- bioluminescence and chemiluminescence research
- Advanced biosensing and bioanalysis techniques
- Nuclear Physics and Applications
- Gene Regulatory Network Analysis
- RNA Interference and Gene Delivery
- Photoreceptor and optogenetics research
- Hepatitis C virus research
- COVID-19 diagnosis using AI
- Nuclear reactor physics and engineering
- Inflammasome and immune disorders
- Bone Metabolism and Diseases
- Heme Oxygenase-1 and Carbon Monoxide
- Hepatitis Viruses Studies and Epidemiology
- Enzyme Catalysis and Immobilization
- Developmental Biology and Gene Regulation
- Radioactivity and Radon Measurements
- Retinal Development and Disorders
- Immune Cell Function and Interaction
- Cell Image Analysis Techniques
Dalian Medical University
2025
Chongqing Medical University
2025
Second Affiliated Hospital of Chongqing Medical University
2025
Guangzhou Eighth People's Hospital
2024
Guangzhou Medical University
2024
Anhui Medical University
2024
Jinan University
2009-2023
Southern University of Science and Technology
2022-2023
Fifth Affiliated Hospital of Sun Yat-sen University
2009-2023
Princeton University
2014-2023
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro vivo. However, generalized protocols FRET standards ensure the reproducibility accuracy measurements efficiencies are currently lacking. Here we report results a comparative blind study which 20 labs determined (E) several dye-labeled DNA duplexes. Using unified, straightforward method, obtained with s.d. between ±0.02 ±0.05. We...
Nanoparticles have been widely applied as gene carrier for improving RNA interference (RNAi) efficiency in medical and agricultural fields. However, the mechanism delivery process of nanoparticle-mediated RNAi is not directly visualized elucidated. Here we synthesized a star polymer (SPc) consisted hydrophilic shell with positively-charged tertiary amine side chain, which was taken an example to investigate delivery. The SPc could assemble dsRNA spontaneously through electrostatic force,...
Despite functional evidence for disulfide bond-reducing activity in endosomal compartments, the mechanistic details pertaining to such process (e.g., kinetics and sites of reduction) remain largely controversial. To address these questions directly, we have synthesized a previously uncharacterized fluorescent folate conjugate, folate-(BODIPY FL)-SS-rhodamine (folate-FRET), that changes fluorescence from red green upon bond reduction. Using this construct, observed reduction: (i) occurs with...
In vivo cross-linking studies suggest that the Drosophila transcription factor Bicoid (Bcd) binds to several thousand sites during early embryogenesis, but it is not clear how many of these binding events are functionally important. contrast, reporter gene have identified >60 Bcd-dependent enhancers, all which contain clusters consensus sequence TAATCC. These also TAATCC motifs (inactive fragments) failed drive activation. general, active fragments showed higher levels Bcd in and were...
Summary Background Coronavirus Disease 2019 (COVID-19) caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global threat to public health. Aiming construct an efficient screening pattern, we comprehensively evaluated the performances of RT-PCR and chest CT in diagnosing COVID-19. Methods The records including demographics, RT-PCR, from 87 confirmed COVID-19 cases 481 exclusion were collected. diagnostic accuracy pharyngeal swab CT, combination with second or...
Background/Aims: Postmenopausal osteoporosis is closely associated with reduction in the differentiation of mesenchymal stem cells (MSCs) into osteoblasts. Previous studies have demonstrated that miR-214 plays an important role genesis and development postmenopausal osteoporosis. Here, we performed this study to investigate potential mechanism by which regulates osteoblast MSCs. Methods: First, explored expression MSCs osteoporotic mice. Next, examined change during Then, were infected...
The final identity and functional properties of a neuron are specified by terminal differentiation genes, which controlled specific motifs in compact regulatory regions. To determine how these sequences integrate inputs from transcription factors that specify cell types, we compared the mechanism Drosophila Rhodopsin genes expressed subsets photoreceptors to phototransduction broadly, all photoreceptors. Both sets share an 11-base pair (bp) activator motif. Broadly contain palindromic...
Skeletal muscle atrophy with high prevalence can induce weakness and fatigability place huge burden on both health quality of life. During skeletal degeneration, excessive fibroblasts extracellular matrix (ECM) accumulated to replace impair the resident fiber led loss mass. Cyclooxygenase-2 (COX-2), rate-limiting enzyme in synthesis prostaglandin, has been identified as a positive regulator pathophysiological process like inflammation oxidative stress. In our study, we found injured muscles...
Lung cancer is the most diagnosed malignant and leading cause of cancer-related deaths worldwide, with advanced stage metastasis being a major issue. The mechanism to not yet understood. Here, we found that KRT16 upregulated in metastatic lung tissues correlated poor overall survival. Knockdown inhibits both vitro vivo. Mechanistically, interacts vimentin, depletion leads downregulation vimentin. acquired its oncogenic ability by stabilizing vimentin required for KRT16-driven metastasis....
Nod-like receptor protein 3 (NLRP3), absent in melanoma 2 (AIM2), and interferon gamma inducible 16 (IFI16) are innate immune sensors for intracellular microbes, which can be activated by various dangerous signals subsequently lead to caspase-1 (CASP1) activation the maturation cleavage of effector molecules pro-IL-1β pro-IL-18. Their roles immunopathology acute chronic hepatitis B virus (HBV) infection still unclear. In this study, we first investigated NLRP3, AIM2, IFI16 inflammasomes...
Background: Coronavirus Disease 2019 (COVID-19) caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global threat to public health. Aiming construct an efficient screening pattern, we comprehensively evaluated the performances of RT-PCR and chest CT in diagnosing COVID-19.Methods: The records including demographics, RT-PCR, from 87 confirmed COVID-19 cases 481 exclusion were collected. diagnostic accuracy pharyngeal swab CT, combination with second or...
Mesenchymal stem cells (MSCs) are able to differentiate into hepatocytes, promote the regeneration of hepatic and inhibit progression fibrosis. Transforming growth factor (TGF)-β1 is one key factors in development liver fibrosis, which also promotes extracellular matrix (ECM) formation. Drosophila mothers against decapentaplegic 7 (Smad7) an essential negative regulator TGF-β1/Smad signaling pathway. In present study, bone mesenchymal (BMSCs) were isolated from rat marrow transfected with...
Chromosomes in the eukaryotic nucleus are highly compacted. However, for many functional processes, including transcription initiation, 3D pair-wise motion of distal chromosomal elements, such as enhancers and promoters, is essential necessitates dynamic fluidity. Therefore, interplay chromosome organization dynamics crucial gene regulation. Here, we use a live imaging assay to simultaneously measure positions pairs promoters their transcriptional output developing fly embryo while...
Aeromonas dhakensis is an important ubiquitous Gram-negative and freshwater bacterium detected in different reservoirs. It can cause invasive diseases humans. Herein, we report the first case Mainland China of a fulminant death 29-year-old man as result new, unexpected association between septicemic A. hepatitis B viral infection (HBV). patient died from multiple organ failure 5 d postadmission after ingestion Snakehead Fish meal. The isolated was initially misidentified hydrophila using...