A5062656088- Aortic aneurysm repair treatments
- Endoplasmic Reticulum Stress and Disease
- Aortic Disease and Treatment Approaches
- Spinal Cord Injury Research
- Melanoma and MAPK Pathways
- Infectious Aortic and Vascular Conditions
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Diet, Metabolism, and Disease
- Macrophage Migration Inhibitory Factor
- Helicobacter pylori-related gastroenterology studies
- Nerve injury and regeneration
- Osteoarthritis Treatment and Mechanisms
- Histone Deacetylase Inhibitors Research
- Ginkgo biloba and Cashew Applications
- Angiogenesis and VEGF in Cancer
- Acute Kidney Injury Research
- Signaling Pathways in Disease
- Nitric Oxide and Endothelin Effects
- NF-κB Signaling Pathways
- Advanced Glycation End Products research
- Neurological Disease Mechanisms and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Tryptophan and brain disorders
- Inflammasome and immune disorders
Nanjing Medical University
2014-2023
Nanjing General Hospital of Nanjing Military Command
2021
Jiangsu Province Hospital
2018
Singapore General Hospital
2016
Institute for Infocomm Research
2016
Xiaomi (China)
2014
The Wistar Institute
2012
Zhejiang University
2007
Sir Run Run Shaw Hospital
2007
Xuzhou Medical College
2007
To investigate the roles of melanoma-associated macrophages in melanoma resistance to BRAF inhibitors (BRAFi).An vitro macrophage and cell coculture system was used whether play a role BRAFi. The effects tumor were examined by proliferation assay, death Western blot analyses. Furthermore, two mouse preclinical models validate targeting can increase antitumor activity Finally, number tissues immunohistochemistry.We demonstrate that BRAF-mutant melanomas, BRAFi paradoxically activate...
BACKGROUND Angiogenesis plays a crucial role in myocardial infarction (MI) treatment by ameliorating remodeling, thus improving cardiac function and preventing heart failure. Muscone has been reported to have beneficial effects on remodeling MI mice. However, the of muscone angiogenesis mice its underlying mechanisms remain unknown. MATERIAL AND METHODS Mice were randomly divided into sham, MI, MI+muscone groups. The mouse model was established ligating left anterior descending coronary...
We examined the pathogenic significance of VEGF (vascular endothelial growth factor)-A in experimental abdominal aortic aneurysms (AAAs) and translational value pharmacological VEGF-A or its receptor inhibition aneurysm suppression. Approaches Results: AAAs were created male C57BL/6J mice via intra-aortic elastase infusion. Soluble VEGFR (VEGF receptor)-2 extracellular ligand-binding domain (delivered Ad [adenovirus]-VEGFR-2), anti-VEGF-A mAb (monoclonal antibody), sunitinib used to...
The presence of tumor-associated macrophages (TAMs) in melanomas is correlated with a poor clinical prognosis. However, there limited information on the characteristics and biological activities human TAMs melanomas. In this study, we developed an vitro method to differentiate monocytes using modified melanoma-conditioned medium (MCM). We demonstrate that factors from MCM-induced (MCMI-Mφ) express both M1-Mφ M2-Mφ markers inhibit melanoma-specific T-cell proliferation. Furthermore,...
Background/Aims: Postmenopausal osteoporosis is closely associated with reduction in the differentiation of mesenchymal stem cells (MSCs) into osteoblasts. Previous studies have demonstrated that miR-214 plays an important role genesis and development postmenopausal osteoporosis. Here, we performed this study to investigate potential mechanism by which regulates osteoblast MSCs. Methods: First, explored expression MSCs osteoporotic mice. Next, examined change during Then, were infected...
Abstract Background Osteoarthritis (OA) is a major cause of limb dysfunction, and distraction arthroplasty which generates intermittent hydrostatic pressure (IHP) an effective approach for OA treatment. However, the result was not always satisfactory reasons remained unresolved. Because aging recognized as important risk factor chondrogenic progenitor cells (CPCs) could acquire senescent phenotype, we made hypothesis that CPCs senescence have harmful effect on chondrogenesis outcome be...
Dehydroepiandrosterone (DHEA) has been implicated not only to prevent N-methyl-d-aspartate (NMDA)-induced neurotoxicity but also enhance Ca 2+ influx through NMDA receptor (NMDAr). However, these DHEA effects, which would produce inconsistent outcomes about neuronal damages, are well studied in ischemia-induced cerebral damages. Herein, we report that a single administration of (20 mg/kg) during 3 48 h after transient global ischemia rats exerted neuroprotective effects such as reduction...
ObjectiveTo investigate the anatomical and histological features of spinal nerve roots provide base data for neuroanastomosis therapy paraplegia.MethodsSpinal from C1 to S5 were exposed on six adult cadavers. The diameter number fibers each root measured, respectively, with a caliper image analysis software.ResultsAs ventral roots, C5 (2.50 ± 0.55 mm) was largest in cervical segments. In thoracic lumbosacral segments, gradually increased T11 S1 then decreased except L3. (1.43 0.16 thickest...
Endoplasmic reticulum (ER) stress-mediated cell apoptosis has been implicated in various types, including fibroblasts. Previous studies have shown that mitomycin C (MMC)-induced occurs fibroblasts, but the effects of MMC on ER fibroblasts not examined. Here, MMC-induced human primary was investigated by exposing cells to a single dose for 5 minutes. Significant inhibition proliferation and increased were observed using viability assay, Annexin V/propidium iodide double staining, cycle...
This study aimed to determine the effect of fibronectin (FN) on cartilage regeneration through activation chondrogenic progenitor cells (CPCs). Cells were isolated from knee mice and cultured in presence various concentrations FN. Proliferation, migration, differentiation assays performed vitro. In some experiments, CPCs preincubated with anti-integrin α5β1 antibody for 60 min before FN treatment block integrin receptor. Soluble was mixed Pluronic F-127 injected into joint cavity an...
It is well recognized that the incidence of heart failure and risk death high in diabetic patients after myocardial infarction (MI). Accumulating evidence showed puerarin (PUE) has protecting function on both cardiovascular disease diabetes. The aim this study to explore whether could improve cardiac mice MI underlying mechanism. left anterior Streptozotocin (STZ)-Nicotinamide (NA) induced were ligated permanently except for Shame group. Then operated randomly treated with PUE or saline....
Depression is common in patients with myocardial infarction (MI), attributing to worse outcomes. Inflammatory response the central nervous system (CNS) considered be a potential mechanism underlying MI induced depression. Our former research demonstrated Ginkgo biloba extract played an important role repression of hippocampus inflammation heart failure mice depressive behaviors. This study was designed investigate effect Ginkgolide B (GB) on MI-induced depression-like behaviors post mice....
Background/Aims: Epidural fibrosis, a common complication after laminectomy, has been demonstrated to be closely associated with poor surgical outcomes. Previous studies showed that taurine had remarkable anti-fibrotic effects on lung and liver fibrosis. We performed this study investigate the of in rat models epidural fibrosis laminectomy explore potential molecular mechanism. Methods: Laminectomy was each establish model. After treatment, Masson's trichrome immunohistochemistry staining...
Background The protective effects of polyamines on cardiovascular disease have been demonstrated in many studies. However, the roles spermidine, a natural polyamine, abdominal aortic aneurysm (AAA) not studied. In this study, we investigated influence and potential mechanisms spermidine treatment experimental AAA disease. Methods Results Experimental AAAs were induced 8- to 10-week-old male C57BL/6J mice by transient intra-aortic infusion porcine pancreatic elastase. Spermidine was...
Purpose.: To evaluate the role of miR-200b expression in proliferation human Tenon's capsule fibroblasts (HTFs) induced by transforming growth factor-beta 1 (TGF-β1). Methods.: Human were treated with various doses TGF-β1 for 24 hours. Cell was quantified cell counting kit-8 (cck-8) assay, cycle analysis, 5-ethynyl-2-deoxyuridine (EdU) and analysis cyclin E, D1, proliferating nuclear antigen (PCNA) expression. MicroRNA-200b (miR-200b) detected quantitative reverse-transcriptase polymerase...
Background: Hepatocyte growth factor (HGF) is widely known as a protective in ischemic myocardium, however HGF sensitive cellular mechanism remained ill-defined. Autophagy at early stage of hypoxia has been demonstrated to play role protecting myocardium both vivo and vitro. We performed this study investigate the association between effect autophagy. Methods: Ventricular myocytes were isolated from neonatal rat heart (NRVMs). evaluated cardiomyocytes apoptosis by Hoechst staining flow...
Ischemia causes desquamation of proximal tubular epithelial cells leading to acute renal failure. However, the molecular mechanisms underlying detachment tubule remain unknown. In this study, we reported that ATP depletion resulted in actin polymerization, a shift filamentous from web-like structure fragmented parallel stress fibers, followed by reduction cellular adhesion ability. The pre-treatment with Jasplakinolide, an stabilizer, prevented depletion-induced polymerization and cell...