A5006070284- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Cancer Research and Treatments
- RNA modifications and cancer
- Advanced Proteomics Techniques and Applications
- Immune Cell Function and Interaction
- Heat shock proteins research
- vaccines and immunoinformatics approaches
- Climate change impacts on agriculture
- Pancreatic function and diabetes
- Mathematical Biology Tumor Growth
- Neuroinflammation and Neurodegeneration Mechanisms
- Liver physiology and pathology
- Autophagy in Disease and Therapy
- Viral Infections and Immunology Research
- Neonatal Respiratory Health Research
- Chemokine receptors and signaling
- Agriculture Sustainability and Environmental Impact
- Tissue Engineering and Regenerative Medicine
Fudan University
2006-2024
Beijing Normal University
2024
Zhongshan Hospital
2006-2024
Second Military Medical University
2003-2023
Changhai Hospital
2023
Beihang University
2020-2022
Eastern Hepatobiliary Surgery Hospital
2018-2022
Shanghai Institute of Nutrition and Health
2021
Chinese Academy of Sciences
2011-2021
Shanghai Institutes for Biological Sciences
2021
The prevalent DNA modification in higher organisms is the methylation of cytosine to 5-methylcytosine (5mC), which partially converted 5-hydroxymethylcytosine (5hmC) by Tet (ten eleven translocation) family dioxygenases. Despite their importance epigenetic regulation, it unclear how these modifications are reversed. Here, we demonstrate that 5mC and 5hmC oxidized 5-carboxylcytosine (5caC) dioxygenases vitro cultured cells. 5caC specifically recognized excised thymine-DNA glycosylase (TDG)....
Abstract Preoperative MRI is one of the most important clinical results for diagnosis and treatment glioma patients. The objective this study was to construct a stable validatable preoperative T2-weighted MRI-based radiomics model predicting survival gliomas. A total 652 patients across three independent cohorts were covered in including their images, RNA-seq data. Radiomic features (1731) extracted from images 167 gliomas (discovery cohort) collected Beijing Tiantan Hospital then used...
Methylation of the fifth position cytosine (5mC) is an important epigenetic modification DNA. It has been shown that oxidized derivatives 5mC, namely 5‐hydroxymethylcytosine (5hmC), 5‐formylcytosine (5fC), and 5‐carboxylcytosine (5caC), are in dynamic existence have distinct regulatory functions. In current study, we investigated whether there changes contents all three 5mC‐oxidized hepatocellular carcinoma (HCC) genome further explored underlying mechanisms. We showed both global genomic...
Abstract The negative signal provided by interactions of programmed death-1 (PD-1) and its ligands, costimulatory molecules PD-L1 (also B7-H1) PD-L2 B7-DC), is involved in the mechanisms tumor immune evasion. In this study, we found that was also evasion immunotherapy. When used different doses a constructed eukaryotic expression plasmid, pSLC, which expresses functional murine secondary lymphoid tissue chemokine (SLC, CCL21), to treat BALB/c mice inoculated with H22 hepatoma cells,...
The popularity of costume dramas has driven the adaptation historical novels, yet fiction elements within these novels pose significant challenges for adapters. Many current TV adapted from fictional works are set in specific periods to address complexities adapting fiction. However, series Nirvana Fire distinguished itself by preserving alternate setting its source material, while still being recognized as a high-quality drama audiences. This paper examines innovative strategies employed...
Abstract Many tumor immunotherapy efforts are focused on the generation of strong T‐cell response against antigens. However, does not always coincide with rejection, for which upregulated expression immunoinhibitory molecules may be responsible. In this study, treatment heat shock protein 70 (HSP70) vaccine induced an infiltration T cells into site as well IFN‐γ and IL‐2, delayed lung metastases tumor, but progression nonetheless occur finally. We demonstrated that B7‐H1 expressed by...
Toll-like receptor 4 (TLR4) specifically recognizes lipopolysaccharide (LPS) to initiate signal transduction events that modulate host inflammatory responses. Although increasing numbers of genes have been characterized individually for their involvement in TLR4 signaling, the LPS-induced TLR4-mediated signaling pathway and connected networks are incompletely delineated. Given most components pathways activated at an early phase response, we employed a subcellular, SILAC-based quantitative...
Neoantigens are mutation-containing immunogenic peptides from tumor cells. Neoantigen intrinsic features neoantigens' sequence-associated characterized by different amino acid descriptors and physical-chemical properties, which have a crucial function in prioritization of neoantigens with potentials predicting patients better survival. Different might functions to varying degrees evaluating immunogenicity. Identification comparison among particularly important for developing neoantigen-based...
In mammalian cells, when tandem affinity purification approach is employed, the existence of untagged endogenous target protein and repetitive washing steps together result in overall low yield purified/stable complexes loss weakly transiently interacting partners biological significance. To avoid trade-offs involving methodological sensitivity, precision, throughput, here we introduce an integrated method, biotin tagging coupled with amino acid-coded mass tagging, for highly sensitive...
Precise and comprehensive identifications of the proteins associated with metastasis are critical for early diagnosis therapeutic intervention hepatocellular carcinoma (HCC). Therefore, we investigated proteomic differences between a pair HCC cell lines, originating from same progenitor, different potential using amino acid-coded mass tagging-based LC-MS/MS quantitative approach. Totally relative abundance 336 in these lines were quantified, which 121 upregulated by >30%, 64 downregulated...
In addition to the important roles played by 5-methylcytosine (5mC), emerging evidence suggests that 5mC derivatives, such as 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), also exhibit regulatory functions in physiological pathological processes. Four cytosine modifications (5mC, 5hmC, 5fC 5caC) are produced erased a cyclic enzymatic cascade mediated DNA methyltransferases (DNMTs), ten-eleven translocation (TET) family enzymes thymine glycosylase...
Liver stem/progenitor cells play a key role in liver development and maybe also cancer development. In our previous study population of c-Kit-(CD45/TER119)- mouse fetal liver, was successfully sorted with large amount (10(6)-10(7)) by using immuno-magnetic microbeads. this study, the were used for proteomic study. Proteins unsorted investigated two-dimensional electrophoresis. A proteome map obtained first time. that exhibited significantly upregulation identified peptide mass fingerprinting...
Cancer stem cells (CSCs) usually account for a very small tumor cell population but play pivotal roles in human cancer development and recurrence. A fundamental question biology is what genetic epigenetic changes occur CSCs. Here we show that the in-situ global levels of DNA cytosine modifications, including 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) 5-formylcytosine (5fC), are similar between liver stem-like (LCSL) paratumor patients. We then developed robust method combining...
AIM:To develop a method to isolate liver stem cells fast and efficiently. METHODS:Fetal mouse were characterized by cell surface antigens (c-Kit CD45/TER119) using flow cytometry.The candidate sorted immuno-magnetic microbeads identified clone-forming culture, RT-PCR immunofluorescence assays. RESULTS:The c-Kit -(CD45/TER119) -cell population with 97.9% of purity purified at one time.The yield this separation was about 6% the total sorting viability above 98%.When cultured in vitro these had...
Abstract Background Neoantigen based personalized immune therapies achieve promising results in melanoma and lung cancer, but few neoantigen models perform well IDH wild-type GBM, the association between intrinsic features prognosis remain unclear GBM. We presented a novel feature-based deep learning model (neoDL) to stratify GBMs into subgroups with different survivals. Results first derived for each associated survival, followed by applying neoDL TCGA data cohort(AUC = 0.988, p value <...
A rapid and convenient dissolvable gel-based one-tube sample treatment method was developed for S -acylation proteomics by combining a tube gel the classic ABE to simplify operations shorten duration.
Abstract This study was to explore the activation of mast cells by microbubbles, with focus on transient receptor potential (TRP) channels mediated degranulation and calcium influx. Bone marrow‐derived (BMMCs) were primarily obtained from femurs in mice induced differentiation for 4 weeks. After purity identification, BMMCs contacted homogeneous microbubbles diameter 1 mm h. β‐hexosaminidase histamine levels supernatants assessed enzyme‐linked immunosorbent assay (ELISA) CD63 expression...