A5030954903- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Ubiquitin and proteasome pathways
- Electrochemical sensors and biosensors
- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- Graphene and Nanomaterials Applications
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Advanced Sensor and Energy Harvesting Materials
- Chemical Synthesis and Analysis
- 14-3-3 protein interactions
- Click Chemistry and Applications
- Metabolomics and Mass Spectrometry Studies
- Dielectric materials and actuators
- Biotin and Related Studies
- Analytical Chemistry and Chromatography
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Proteoglycans and glycosaminoglycans research
- Machine Learning in Bioinformatics
Fudan University
2011-2025
Xinjiang University
2024
Hulunbuir University
2024
Zhongshan Hospital
2004-2024
Fudan University Shanghai Cancer Center
2017-2022
Xi'an International Studies University
2022
Jilin University
2021
Tianjin University of Traditional Chinese Medicine
2017
University of Massachusetts Amherst
2015
University of North Carolina at Chapel Hill
2009
Abstract Conductive nanofibers are adopted to enhance the electric properties of ferroelectric polymers. Polyaniline (PANI) doped by protonic acids have a high dispersion stability in vinylidene fluoride‐trifluoroethylene copolymers [P(VDF‐TrFE)] and lead percolative nanocomposites with enhanced responses. About 50‐fold rise dielectric constant polymer matrix has been achieved. Percolation thresholds relevant doping levels PANI can be as low 2.9 wt% for fully nanofibers. The interface...
Sialylated N-glycan isomers with α-2,3 and α-2,6 linkages play crucial distinctive roles in diverse physiological pathological processes. Changes of α-2,3-linked sialic acids sialylated N-glycans are especially important monitoring the initiation progression diseases. However, specific analysis α-2,3-sialylated linkage remains challenging due to their extremely low abundance technical limitations separation detection. Herein, we designed an integrated strategy that combines linkage-specific...
In this report, trypsin was immobilized in the layer-by-layer (LBL) coating of graphene oxide (GO) and chitosan on a piece glass fiber to fabricate microchip bioreactor for efficient proteolysis. LBL deposition driven by electrostatic forces easily took place surface fiber, providing mild environmental conditions so that denaturation autolysis minimized. Prior use, prepared trypsin-immobilized inserted into channel form core-changeable bioreactor. The novel GO-based can be regenerated...
Sialylated N-glycans play pivotal role in several important biological and pathological processes. Their sialyl-linkage isomers, mostly α-2,3- α-2,6-linked, act differently during the cellular events diseases. While mass spectrometry (MS) technology is a powerful tool N-glycome analysis, it still suffers from an inability to distinguish linkage isomers of native N-glycans. Herein, we described sequential selective derivatization method, by which α-2,6- α-2,3-linked sialic acids are...
In this report, infrared (IR)-assisted on-plate proteolysis has been developed for rapid peptide mapping. Protein solutions containing trypsin were allowed to digest directly on the spots of matrix-assisted laser desorption/ionization (MALDI) plates under IR radiation. The feasibility and performance novel approach investigated by digestion bovine serum albumin (BSA) cytochrome c (Cyt-c). It was demonstrated that radiation substantially enhanced efficiency time significantly reduced 5 min....
Abstract A new approach based on far infrared‐assisted in situ reduction was developed for the facile one‐step preparation of graphene–nickel nanoparticle hybrid by refluxing a mixture solution containing graphene oxide, nickel(II) sulfate, and hydrazine over an far‐infrared heater. The time as short 20 min. structure material investigated transmission electron microscopy, scanning X‐ray diffraction, energy dispersive spectroscopy, vibrating sample magnetometery, Fourier transform infrared...
Modification of proteins with 4-hydroxynonenal (HNE) is known to alter the function and regulate associated biological processes in eukaryotic cells. The development mass spectrometry (MS) makes high-throughput analysis HNE modification accessible. However, identification still hampered by low frequency this modification. Therefore, only a limited number sites have been identified. enrichment HNE-modified peptides critical for MS because its abundance. Herein, we explored novel strategy...
The replacement histone variant H2AX senses DNA double-strand breaks (DSBs) and recruits characteristic sets of proteins at its phosphorylated (gamma-H2AX) foci for concurrent repair. We reasoned that the interaction network, or interactome, formed in tumor-associated DSB environment such as hepatocellular carcinoma (HCC) cells, where preneoplastic lesions frequently occur, is indicative HCC pathogenic status. By using an vivo dual-tagging quantitative proteomic method, we identified 102...
In this report, infrared (IR) radiation was employed to enhance the efficiency of in-gel proteolysis for MS-based protein identification. After sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), target bands excised from polyacrylamide were cut into small pieces that further treated in trypsin solution. Subsequently, wet sealed transparent Eppendorf tubes exposed an IR lamp perform IR-assisted digestion. To demonstrate feasibility and performance novel digestion approach,...
Abstract We report for the first time an expressed proteome human hepatocellular carcinoma (HCC) in nude mice model. Most cases of liver cancer are HCC with highly metastatic ability. Therefore, early prediction or diagnosis and effective treatment key points research. have previously successfully established a model (LCI‐D20) high metastasis potential. To understand better tumor biology it is worth to explore relativity all protein profiles LCI‐D20 With advanced proteomics technologies, we...
Abstract To fabricate ferroelectric ultrathin polymer films with large dielectric constants for potential all‐organic electronic devices, nanotubes and a composite of the dispersed organic semiconductor have been fabricated by template‐assisted methods. The ferroelectricity drops markedly in spin‐coated less than 100 nm thick, whereas P(VDF‐TrFE) wall thickness few ten sustain ferroelectricity. exhibit giant constant as result significantly enhanced interface polarization between nanosized...
Analysis of protein C termini is very important for functional annotations proteomes, while proteome-wide analysis still poses substantial challenges. Here we described a simple and robust strategy specific isolation based on LysC digestion site-selective dimethylation to deplete N-terminal internal peptides by scavenger materials. The performance conditions resin coupling were discussed in detail. Then the was successfully applied characterization HeLa cells. A total 781 identified with 300...
Abstract A novel proteolysis approach was developed by using infrared (IR) radiation and trypsin‐immobilized silica microspheres. Protein solutions containing microspheres in sealed transparent Eppendorf tubes were allowed to digest under an IR lamp at 37°C. The feasibility performance of the present demonstrated digestion BSA cytochrome c (Cyt‐ ) time significantly reduced 5 min. obtained digests identified MALDI‐TOF‐MS with sequence coverages 54% (BSA) 83% that better than those...
Lack of understanding the response hepatocellular carcinoma (HCC) to anticancer drugs causes high mortality HCC patients. Bleomycin (BLM) that induces DNA damage is clinically used for cancer therapy, while mechanism underlying BLM-induced (DDR) in cells remains ambiguous. Given 14-3-3 proteins are broadly involved regulation diverse biological processes (BPs)/pathways, we investigate how a isoform coordinates particular BPs/pathways DDR HCC.Using dual-tagging quantitative proteomic...
Abstract Protein post‐translational modifications (PTMs) are chemical on proteins. PTMs play a key role in many cellular processes by influencing the structure of proteins and dynamically regulating their functions. Therefore, characterizing at proteome level is critical to provide invaluable insight into functions underlying different biological processes. Advances modern proteomics technologies including sample preparation, chromatography separation as well mass spectrometry have propelled...
Protein glycosylation, the most universal post-translational modification, is thought to play a crucial role in regulating multiple essential cellular processes. However, low abundance of glycoproteins and heterogeneity glycans complicate their comprehensive analysis. Here, we develop rapid large-scale glycopeptide enrichment strategy via bioorthogonal ligation trypsin cleavage. The process performed one tube minimize sample loss time costs. This method combines convenience practicality,...