A5101554108- Advanced Proteomics Techniques and Applications
- Plant Stress Responses and Tolerance
- Mass Spectrometry Techniques and Applications
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Mosquito-borne diseases and control
- Glycosylation and Glycoproteins Research
- Herpesvirus Infections and Treatments
- Mycorrhizal Fungi and Plant Interactions
- S100 Proteins and Annexins
- Ruminant Nutrition and Digestive Physiology
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Aluminum toxicity and tolerance in plants and animals
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Animal Nutrition and Physiology
- Vector-Borne Animal Diseases
- Enzyme Structure and Function
Yunnan Animal Science and Veterinary Institute
2012-2025
Shaanxi University of Science and Technology
2025
Wuhan University
2025
Fudan University
2013-2024
Beijing Forestry University
2015-2024
Taiyuan Central Hospital
2024
Guangdong Academy of Forestry
2021-2024
China University of Geosciences (Beijing)
2017-2024
Second Affiliated Hospital of Soochow University
2024
The Fourth Affiliated Hospital of Soochow University (Suzhou Dushu Lake Hospital)
2024
Metabolic Regulation Through Acetylation Covalent modification of lysine residues in various proteins the nucleus is a recognized mechanism for control transcription. Now two papers suggest that acetylation may represent an important regulatory controlling function metabolic enzymes (see Perspective by Norvell and McMahon ). Zhao et al. (p. 1000 ) found large proportion pathways were acetylated human liver cells. regulated distinct mechanisms, directly activating some, inhibiting one,...
Lysine acetylation regulates many eukaryotic cellular processes, but its function in prokaryotes is largely unknown. We demonstrated that central metabolism enzymes Salmonella were acetylated extensively and differentially response to different carbon sources, concomitantly with changes cell growth metabolic flux. The relative activities of key controlling the direction glycolysis versus gluconeogenesis branching between citrate cycle glyoxylate bypass all regulated by acetylation. This...
Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression P. roots and leaves. We isolated 2022 bp promoter fragment upstream the translational start from euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased root, leaf, stem tissues salt stress. DNA affinity...
Abstract Tall fescue ( Festuca arundinacea Schreb ) is a turf grass species which widely used for rhizoremediation of organic contaminants and shows notable prospects in heavy metal phytoremediation. In this study, different concentrations cadmium ion (Cd 2+ were applied to study toxic effects Cd responses tall by soilless culture. showed comparable high tolerance as Indian mustard Brassica juncea L.). Additionally, the treatment with concentration leaded decreased chlorophyll contents,...
Salt stress is an important environmental cue impeding poplar nitrogen nutrition. Here, we characterized the impact of salinity on proton-driven nitrate fluxes in ectomycorrhizal roots and importance a Hartig net for uptake. We employed two Paxillus involutus strains root colonization: MAJ, which forms typical structures (mantle net), NAU, colonizing with thin, loose hyphal sheath. Fungus-colonized noncolonized Populus × canescens were exposed to sodium chloride used measure surface pH,...
Porcine pseudorabies has long existed in China and is a serious threat to the Chinese farming industry. To understand prevalence genetic variation of porcine virus (PRV) its pathogenicity Yunnan Province, China, we collected 560 serum samples across seven Province regions from 2020 2021 detected anti-gE antibodies these samples. Sixty-one clinical tissue were also pigs with suspected PRV that vaccinated Bartha-K61. PRV-gE found 29.6% (166/560) The positivity rate was 13.1% (8/61). Two...
Abstract Chronic inflammation is pivotal in the pathogenesis of hepatocellular carcinoma (HCC). Histamine a biologically active substance that amplifies inflammatory and immune response serves as neurotransmitter. However, knowledge histamine’s role HCC its effects on immunotherapy remains lacking. We focused histamine-related genes to investigate their potential HCC. The RNA-seq data clinical information regarding were obtained from Cancer Genome Atlas (TCGA). After identifying...
Porcine epidemic diarrhea virus (PEDV) is a highly contagious pathogen responsible for devastating enteric disease and lethal watery diarrhea, leading to significant economic losses in the global swine industry. Understanding epidemiology genetic diversity of PEDV over past decade crucial effective prevention treatment porcine diarrhea. In this study, 1851 fecal samples were collected from pigs exhibiting symptoms across 11 cities Yunnan Province between 2013 2022. The prevalence PEDV, along...
Abstract The proteins involved in breast cancer initiation and progression are still largely elusive. To gain insights into these processes, we conducted quantitative proteomic analyses with 21T series of cell lines, which include a normal, primary tumor metastatic that were isolated from single patient. Stable isotope labeling amino acid culture followed by LC‐MS/MS analysis was performed deregulated identified using statistical analysis. Gene ontology revealed metabolic processes the most...
Ornithine carbamoyltransferase (OTC) is a key enzyme in the urea cycle to detoxify ammonium produced from amino acid catabolism. OTC deficiency an X-linked genetic disorder ranging fatal newborns hyperammonemia and anorexia adults. Through affinity purification of acetylated peptides mass spectrometry, we identified that on lysine residues, including Lys88, which also mutated OTC-deficient patients. acetylation was confirmed occur under physiological conditions. Biochemical characterizations...
Previous studies showed that monochromatic green light stimuli during embryogenesis accelerated posthatch body weight (BW) and pectoral muscle growth of broilers. In this experiment, we further investigated the morphological molecular basis phenomenon. Fertile broiler eggs (Arbor Acres, n=880) were pre-weighed randomly assigned to 1 2 incubation treatment groups: (1) dark condition (control group), (2) group (560 nm). The lighting systems sourced from light-emitting diode lamps equalized at...
A total of 192 broiler chicks were used to evaluate the influence dietary α-lipoic acid (ALA) on growth performance, carcass characteristics and meat quality chickens with purpose developing a strategy prevent occurrence pale, soft, exudative (PSE) improve broilers. At 22 d age, birds allocated 4 ALA treatments (0, 400, 800, 1200 ppm). The results showed that significantly decreased average feed intake (AFI), daily gain (ADG), final live body weight (BW) (p<0.05), while no difference in...
Virus-induced gene silencing (VIGS) has been shown to be an effective tool for investigating functions in herbaceous plant species, but rarely tested trees. The establishment of a fast and reliable transformation system is especially important woody plants, many which are recalcitrant transformation. In this study, we established tobacco rattle virus (TRV)-based VIGS two Populus euphratica P. × canescens. Here, TRV constructs carrying 266 bp or 558 fragment the phytoene desaturase (PDS) were...
In recent years, the prevalence of pseudorabies virus (PRV) has caused huge economic losses to Chinese pig industry. Meanwhile, PRV infection in humans also sounded alarm about its cross-species transmission from pigs humans. To study regional epidemic, serological and epidemiological investigations populations Yunnan Province during 2017-2021 were performed. The results showed that 31.37% (6324/20,158, 95% CI 30.73-32.01) serum samples positive for glycoprotein E (gE)-specific antibodies...
S-acylation is a widespread lipidation form in eukaryotes which various fatty acids can be covalently attached to specific cysteine residues. However, due the low reactivity of lipid moieties and lack antibodies, purification intact S-acylated peptides remains challenging. Here, we developed pretreatment method for direct separation global analysis endogenously by nanographite fluoride-based solid-phase extraction (nGF-SPE), together with investigation optimization enrichment procedure as...