A5103277398- RNA Research and Splicing
- Nitric Oxide and Endothelin Effects
- RNA modifications and cancer
- Eicosanoids and Hypertension Pharmacology
- Pain Mechanisms and Treatments
- Cancer-related molecular mechanisms research
- Head and Neck Surgical Oncology
- Neurobiology and Insect Physiology Research
- Cancer, Hypoxia, and Metabolism
- Genomics and Chromatin Dynamics
- Visual perception and processing mechanisms
- Mast cells and histamine
- Cancer Risks and Factors
- Dialysis and Renal Disease Management
- Vascular Malformations Diagnosis and Treatment
- Gout, Hyperuricemia, Uric Acid
- Spinal Dysraphism and Malformations
- Case Reports on Hematomas
- Spinal Hematomas and Complications
- Family and Disability Support Research
- Advanced Fluorescence Microscopy Techniques
- Diagnosis and Treatment of Venous Diseases
- Immune Cell Function and Interaction
- Restless Legs Syndrome Research
- NF-κB Signaling Pathways
Shanghai Institute for Science of Science
2023-2024
Fudan University
2019-2024
Shanghai Center for Brain Science and Brain-Inspired Technology
2023-2024
Stanford University
2013-2023
Henan Cancer Hospital
2020-2023
Zhengzhou University
2020-2023
Jinshan Hospital of Fudan University
2021
Wuhan University
2019
University of California, Los Angeles
2018-2019
Long Island Jewish Medical Center
2015
To achieve guide RNA (gRNA) multiplexing and an efficient delivery of tens distinct gRNAs into single cells, we developed a molecular assembly strategy termed chimeric array gRNA oligonucleotides (CARGO). We coupled CARGO with dCas9 (catalytically dead Cas9) imaging to quantitatively measure the movement enhancers promoters that undergo differentiation-associated activity changes in live embryonic stem cells. Whereas all examined functional elements exhibited subdiffusive behavior, their...
Co-transcriptional pre-mRNA processing relies on reversible phosphorylation of the carboxyl-terminal domain (CTD) Rpb1, largest subunit RNA polymerase II (RNAP II). In this study, we replaced in live cells endogenous Rpb1 by S2A where second serines (Ser2) CTD heptapeptide repeats were switched to alanines, prevent phosphorylation. Although slower, RNAP was able transcribe. However, it failed recruit splicing components such as U2AF65 and U2 snRNA transcription sites, although recruitment U1...
Numerous non-coding RNAs are known to be involved in the regulation of gene expression. In this work, we analyzed that co-immunoprecipitated with human RNA polymerase II from mitotic cell extracts and identified U1 small nuclear (snRNA) as a major species. To investigate possible splicing-independent recruitment snRNA transcription units, established lines having integrated reporter containing functional intron or splicing-deficient construction. Recruitment U snRNAs some splicing factors...
Abstract The proteins involved in breast cancer initiation and progression are still largely elusive. To gain insights into these processes, we conducted quantitative proteomic analyses with 21T series of cell lines, which include a normal, primary tumor metastatic that were isolated from single patient. Stable isotope labeling amino acid culture followed by LC‐MS/MS analysis was performed deregulated identified using statistical analysis. Gene ontology revealed metabolic processes the most...
Aim: Salazosulfapyridine (SASP) frequently causes several adverse reactions, such as drug rash with eosinophilia and systemic symptoms (DRESS). This study aims to assess whether there is an association between SASP-induced DRESS HLA-A, -B -C alleles in the Chinese Han population. Subjects & methods: We performed of six subjects DRESS, 30 SASP-tolerant patients 283 general from human MHC database, all whom are Chinese. Results: The frequency carrying HLA-B*13:01 allele 66.67% (4/6). It...
We have shown that hydrogen peroxide (H 2 O ) downregulates tetrahydrobiopterin salvage enzyme DHFR (dihydrofolate reductase) to result in eNOS (endothelial NO synthase) uncoupling and elevated blood pressure. Here, we aimed delineate molecular mechanisms underlying H downregulation of endothelial by examining transcriptional pathways hypothesized modulate expression effects on pressure regulation targeting these novel mechanisms. dose time dependently attenuated mRNA protein enzymatic...
Abstract Colitis-associated cancer (CAC), a prototype of inflammation-associated cancer, is one the most common gastrointestinal tumors. As potential testis antigen (CT antigen), 55 (CT55) expressed in different tumors and normal testes. However, its role CAC remains unknown. Here, we identified CT55 as new potent promoter CAC. We discovered that Ct55 deficiency alleviated inflammatory responses, decreased cell proliferation colitis-associated tumorigenesis an azoxymethane/dextran sulfate...
Backgrounds. Long noncoding RNAs (lncRNAs) play an important role in various biological processes. However, their functions salt-sensitive hypertension are largely unknown. In this study, the lncRNA-seq technique was employed to compare expression profiles of lncRNAs and mRNAs hypertensive rats. Methods . Blood pressure, serum sodium, urinary creatinine were texted salt-insensitive rats fed with different salt concentrations. High-throughput sequencing used detect mRNA renal medulla two...
Abstract Background The feasibility of submandibular gland (SMG) preservation in oral squamous cell carcinoma (SCC) has occasionally been analyzed, but the differences survival associated with presence or absence SMG remain unknown. We aimed to prospectively evaluate oncologic results cT1-2 N0 buccal SCC. Methods This was a prospective, non-randomized cohort study. Patients surgically treated SCC were enrolled and divided into two groups based on management SMG. Level 1b lymph nodes...
Fusobacterium nucleatum (F. nucleatum) often colonizes cancerous gastric tissues and is characterized by the promotion of platelet aggregation development visceral thrombosis. Venous thromboembolism (VTE) leads to a significant increase in mortality cancer (GC) patients. However, relationship between colonization F. prognosis GC patients still unknown.The aim this study was explore whether related complicated with VTE other potential risk factors.From 2017-2021, data 304 new VTEs during...
Detecting looming signals for collision avoidance encounters challenges in real‐world scenarios, where moving backgrounds can interfere as an agent navigates through complex natural environments. Remarkably, even insects with limited neural systems adeptly respond to stimuli while motion at high speeds. Existing insect‐inspired detection models typically rely on either motion‐pathway or feature‐pathway signals, yet both are susceptible dynamic visual scene interference. Coordinating...
Assessing endurance in non-ambulatory individuals with Spinal Muscular Atrophy (SMA) has been challenging due to limited evaluation tools. The Assisted 6-Minute Cycling Test (A6MCT) is an upper limb ergometer assessment used other neurologic disorders measure endurance. To study the performance of A6MCT SMA population, prospective data was collected on 38 (13 sitters; 25 non-sitters), aged 5 74 years (mean = 30.3; SD 14.1). clinical measures were A6MCT, Revised Upper Limb Module (RULM),...
ABSTRACT Detecting looming signals for collision avoidance faces challenges in real-world scenarios due to interference from moving backgrounds. Astonishingly, animals, like insects with limited neural systems, adeptly respond stimuli while at high speeds. Existing insect-inspired detection models integrate either motion-pathway or feature-pathway signals, remaining susceptible dynamic visual scene interference. We propose that coordinating interneuron the two pathways could elevate...