A5001957696- RNA Research and Splicing
- Hippo pathway signaling and YAP/TAZ
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Protein Kinase Regulation and GTPase Signaling
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Nuclear Structure and Function
- Glioma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Lipid metabolism and biosynthesis
- Cardiomyopathy and Myosin Studies
- 14-3-3 protein interactions
- Galectins and Cancer Biology
- Metabolism, Diabetes, and Cancer
- Wnt/β-catenin signaling in development and cancer
- FOXO transcription factor regulation
- Radiomics and Machine Learning in Medical Imaging
- Glycosylation and Glycoproteins Research
- Cancer-related gene regulation
Fudan University
2015-2024
Huashan Hospital
2016-2024
State Key Laboratory of Medical Neurobiology
2020-2024
Fudan University Shanghai Cancer Center
2020-2023
Shanghai Medical College of Fudan University
2013-2023
Shanghai Cancer Institute
2021-2023
Cancer Institute (WIA)
2021-2023
Zhongshan Hospital
2023
Sun Yat-sen University
2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Abstract The evolutionarily conserved Par3/Par6/aPKC complex regulates the polarity establishment of diverse cell types and distinct polarity-driven functions. However, how Par is concentrated beneath membrane to initiate polarization remains unclear. Here we show that exhibits cycle-dependent condensation in Drosophila neuroblasts, driven by liquid–liquid phase separation. open conformation Par3 undergoes autonomous separation likely due its NTD-mediated oligomerization. Par6, via...
Uneven distribution and local concentration of protein complexes on distinct membrane cortices is a fundamental property in numerous biological processes, including Drosophila neuroblast (NB) asymmetric cell divisions polarity general. In NBs, the fate determinant Numb forms basal crescent together with Pon segregated into daughter to initiate its differentiation. Here we discover that PTB domain, using two binding surfaces, recognizes repeating motifs within previously unrecognized mode....
Abstract HECT E3 ligases control the degradation and functioning of numerous oncogenic/tumor-suppressive factors signaling proteins, their activities must be tightly regulated to prevent cancers other diseases. Here we show that Nedd4 family WWP1 adopts an autoinhibited state, in which its multiple WW domains sequester using a multi-lock mechanism. Removing WW2 or WW34 led partial activation WWP1. The structure fully inhibited reveals many mutations identified cancer patients result...
Tumor growth, especially in the late stage, requires adequate nutrients and rich vasculature, which PKM2 plays a convergent role. It has been reported that PKM2, together with FOXM1D, is upregulated late-stage colorectal cancer associated metastasis; however, their underlying mechanism for promoting tumor progression remains elusive. Herein, we revealed FOXM1D potentiates PKM2-mediated glycolysis angiogenesis through multiple protein-protein interactions. In presence of FBP, binds to...
Lipid homeostasis is essential for normal cellular functions and dysregulation of lipid metabolism highly correlated with human diseases including neurodegenerative diseases. In the ubiquitin-dependent autophagic degradation pathway, Troyer syndrome–related protein Spartin activates recruits HECT-type E3 Itch to droplets (LDs) regulate their turnover. this study, we find that promotes formation condensates independent LDs. through its multiple PPAY-motif platform generated by...
BCAT2-mediated branched-chain amino acid (BCAA) catabolism is critical for pancreatic ductal adenocarcinoma (PDAC) development, especially at an early stage. However, whether a high-BCAA diet promotes PDAC development in vivo, and the underlying mechanism of BCAT2 upregulation, remain undefined. Here, we find that intraepithelial neoplasia (PanIN) progression LSL-KrasG12D/+ ; Pdx1-Cre (KC) mice. Moreover, screened with available deubiquitylase library which contains 31 members USP family...
Rho kinase (ROCK), a downstream effector of GTPase, is serine/threonine protein that regulates many crucial cellular processes via control cytoskeletal structures. The C-terminal PH-C1 tandem ROCKs has been implicated to play an autoinhibitory role by sequestering the N-terminal domain and reducing its activity. binding lipids pleckstrin homology (PH) not only localization but also releases from close conformation thereby activates However, molecular mechanism governing ROCK tandem-mediated...
The double-stranded RNA-binding protein Staufen1 (STAU1) regulates a variety of physiological and pathological events via mediating RNA metabolism. STAU1 overabundance was observed in tissues from mouse models fibroblasts patients with neurodegenerative diseases, accompanied by enhanced mTOR signaling impaired autophagic flux, while the underlying mechanism remains elusive. Here, we find that endogenous forms dynamic cytoplasmic condensate normal tumor cell lines, as well Huntington’s...