A5087773115- Glioma Diagnosis and Treatment
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Genomics and Rare Diseases
- BRCA gene mutations in cancer
- Biological Stains and Phytochemicals
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Meningioma and schwannoma management
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- Prostate Cancer Treatment and Research
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Pituitary Gland Disorders and Treatments
- Plant-Derived Bioactive Compounds
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Bioinformatics and Genomic Networks
- Sarcoma Diagnosis and Treatment
Huashan Hospital
2016-2025
Fudan University
2016-2025
Shanghai Center for Brain Science and Brain-Inspired Technology
2022-2025
Shanghai Clinical Research Center
2022-2025
Karamay Central Hospital of Xinjiang
2025
Southern Medical University
2011-2024
Chongqing Medical and Pharmaceutical College
2022-2024
Linyi People's Hospital
2024
Chongqing Medical University
2015-2024
Children's Hospital of Chongqing Medical University
2024
Glioblastoma cells secrete extra-cellular vesicles (EVs) containing microRNAs (miRNAs). Analysis of these EV miRNAs in the bio-fluids afflicted patients represents a potential platform for biomarker development. However, analytic algorithm quantitative assessment miRNA remains under-developed. Here, we demonstrate that reference transcripts commonly used PCR (including GAPDH, 18S rRNA, and hsa-miR-103) were unreliable assessing miRNA. In this context, quantitated absolute terms normalized...
Abstract Protein corona presents a major obstacle to bench-to-bedside translation of targeted drug delivery systems, severely affecting targeting yields and directing unfavorable biodistribution. Corona-mediated provides new impetus for specific by precisely manipulating interaction modes functional plasma proteins on nano-surface. Here bio-inspired liposomes (SP-sLip) were developed modifying liposomal surface with short nontoxic peptide derived from Aβ 1-42 that specifically interacts the...
Abstract Purpose: The objective of this study was to evaluate clinical significance and immunosuppressive mechanisms B7-H4 (B7x/B7S1), a B7 family member, in glioma. Experimental Design: levels glioma tissue/cerebral spinal fluid (CSF) were compared between different grades patients. Survival data analyzed with Kaplan–Meier determine the prognostic value B7-H4. Cytokines from CD133+ cells stimulate expression on human macrophages (Mϕs) investigated by FACS, neutralizing antibodies, Transwell...
Background Hyperlipidemia-induced apoptosis mediated by fatty acid translocase CD36 is associated with increased uptake of ox-LDL or in macrophages, hepatocytes and proximal tubular epithelial cells, leading to atherosclerosis, liver damage fibrosis obese patients, diabetic nephropathy (DN), respectively. However, the specific role podocyte DN hyperlipidemia remains poorly investigated. Methods The expression was measured paraffin-embedded kidney tissue samples (Ctr = 18, 20)...
To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma.Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray® Human MicroRNA Panel. profiles glioblastoma patients controls created three discovery cohorts confirmed in two validation cohorts.miRNA clinical correlated with those found tissues. Comparison of between non-brain yielded "signature" consisting nine miRNAs. The volume (p=0.008)....
Abstract Background Spontaneous intracerebral hemorrhage (ICH) is a devastating form of stroke with the high mortality twofold to sixfold higher than that for ischemic stroke. But treatment haematomas within basal ganglia continues be matter debate among neurologists and neurosurgeons. The purpose this study judge short-term long-term clinical value minimally invasive stereotactic puncture therapy (MISPT) on acute ICH. Methods A prospective controlled was undertaken. trial in compliance WMA...
BACKGROUND: Tumor specific genetic material can be detected in extracellular vesicles (EVs) isolated from blood, cerebrospinal fluid (CSF), and other biofluids of glioblastoma patients. As such, EVs have emerged as a promising platform for biomarker discovery. However, the optimal procedure to tran sport clinical EV samples remains poorly characterized. METHODS: We examined stability CSF patients that were stored under different conditions. recovery was determined by Nanoparticle tracking...
Since the discovery of mutations in isocitrate dehydrogenase 1 (IDH1) gliomas and other tumors, significant efforts have been made to gain a deeper understanding consequences this oncogenic mutation. One aspect neomorphic function IDH1 R132H enzyme that has received less attention is perturbation cellular redox homeostasis. Here, we describe biosynthetic pathway exhibited by cells expressing mutant IDH1. By virtue change homeostasis, IDH1-mutated synthesize excess glutamine-derived proline...
The available evidence suggests that the lethality of glioblastoma is driven by small subpopulations cells self-renew and exhibit tumorigenicity. It remains unclear whether tumorigenicity exists as a static property few or dynamically acquired property. We used tumor-sphere xenograft formation assays for examined subclones isolated from established primary lines. Our results indicate largely deterministic, yet can be spontaneously at low frequencies. Further, these dynamic transitions are...
The key-stone-pathogen, Porphyromonas gingivalis associates not only with periodontal diseases but a variety of other chronic such as cancer. We previously reported an association between the presence in esophageal squamous cell carcinoma (ESCC) and its progression. now report diagnostic prognostic potential serum immunoglobulin G A antibodies (IgG/A) against for ESCC. An enzyme-linked immunosorbent assay (ELISA) was used to determine levels IgG IgA 96 cases ESCC, 50 esophagitis 80 healthy...
Notch1 regulates tumor biology in a complex, context-dependent manner. The roles of tongue cancer are still controversial. aim this study is to investigate the cancer.The expression was tested between and normal samples by using immunohistochemistry. Tongue cells were transfected with siRNA or plasmid, respectively. Cell proliferation, apoptosis, migration invasion ability appropriate ways. subcutaneous model established observe growth.Notch1 upregulated carcinoma tissues related stage...
BackgroundRecurrence after radiation therapy is nearly universal for glioblastomas, the most common form of adult brain cancer. The study aims to define clinically pertinent mechanisms underlying this recurrence.MethodsmicroRNA (miRNA) profiling was performed using matched pre- and post-radiation treatment glioblastoma specimens from same patients. All harbored unmethylated O6-methylguanine-DNA methyltransferase promoters (umMGMT) wild-type isocitrate dehydrogenase (wtIDH). altered miRNA,...
The purpose of this study was to judge the clinical value minimally invasive stereotactic puncture and thrombolysis therapy (MISPTT) for acute intracerebral hemorrhage (ICH). A randomized control trial undertaken. According enrollment criteria, 122 ICH cases were analyzed, which 64 received MISPTT (MISPTT group, MG) 58 conventional craniotomy (CC CG). Glasgow coma scale (GCS) scores, postoperative complications (PC), rebleeding incidences compared. Moreover, 1 year postoperation, long-term...
Morus, a plant genus from the family of Moraceae, most plants which are used as traditional medicines in Asian counties. The root barks Morus normally called Sang-Bai-Pi (SBP) Chinese and for treatment inflammatory respiratory diseases. Decades research on phytochemistry SBP have led to identification various compounds, such Diels–Alder-type adducts, flavonoids, benzofurans, stilbenes, polyhydroxylated alkaloids, etc. These compounds showed wide range bioactive features including...
The combination of direct sampling ionization and miniature mass spectrometer presents a promising technical pathway point-of-care analysis in clinical applications. In this work, spectrometry system was used for tissue samples. Direct coupled with extraction spray home-built spectrometer, Mini 12. Lipid species samples were well profiled rat brain, kidney, liver couple minutes. By incorporating photochemical (Paternò-Büchi) reaction, fast identification lipid C═C location realized. Relative...
The involvement of propofol and circular RNAs (circRNAs) in lung cancer progression has been identified. However, the relationship between circRNAs as well underlying molecular mechanisms on development remain unclear. Cell viability, migration invasion were measured by cell counting kit-8 assay, 5-bromo-2-deoxyuridine (BrdU) transwell assay. Glycolytic metabolism was calculated measuring glucose consumption, lactate production extracellular acidification. Western blot used to detect protein...
Highlights•TKT attenuates the chemosensitivity in glioma independent of catalytic activity•Chemotherapeutic drugs promote TKT's nuclear translocation cells•TKT interacts with XRN2 to regulate resolution and removal R-loopsSummaryGlioblastoma (GBM) is a highly lethal malignant brain tumor poor survival rates, chemoresistance poses significant challenge treatment patients GBM. Here, we show that transketolase (TKT), metabolic enzyme pentose phosphate pathway (PPP), chemotherapy sensitivity...