A5100428364- Metabolomics and Mass Spectrometry Studies
- Air Quality and Health Impacts
- Atmospheric chemistry and aerosols
- Genetics, Aging, and Longevity in Model Organisms
- Sphingolipid Metabolism and Signaling
- Atmospheric aerosols and clouds
- Computational Drug Discovery Methods
- Extracellular vesicles in disease
- PI3K/AKT/mTOR signaling in cancer
- Protein Structure and Dynamics
- Liver Disease Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Remote Sensing and LiDAR Applications
- 3D Surveying and Cultural Heritage
- Endoplasmic Reticulum Stress and Disease
- Circadian rhythm and melatonin
- Microbial Natural Products and Biosynthesis
- Air Quality Monitoring and Forecasting
- Aquatic Ecosystems and Phytoplankton Dynamics
- Single-cell and spatial transcriptomics
- Nanoparticle-Based Drug Delivery
- Biocrusts and Microbial Ecology
- Marine Sponges and Natural Products
- Marine Biology and Ecology Research
- Lipid metabolism and disorders
Hunan University of Traditional Chinese Medicine
2025
University of Chinese Academy of Sciences
2015-2024
Chinese Academy of Sciences
2014-2024
Beijing National Laboratory for Molecular Sciences
2023-2024
Molecular Sciences Institute
2024
South China Sea Institute Of Oceanology
2015-2024
Institute of Oceanology
2015-2024
Affiliated Hospital of Hangzhou Normal University
2024
BGI Group (China)
2021-2023
Beijing Graphene Institute
2023
Abstract Motivation: Molecular recognition between biological macromolecules and organic small molecules plays an important role in various life processes. Both structural information binding data of biomolecular complexes are indispensable for depicting the underlying mechanism such event. The PDBbind database was created to collect experimentally measured throughout Protein Data Bank (PDB). It thus provides linkage energetic properties complexes, which is especially desirable computational...
Psoriasis is a common and chronic inflammatory skin disease that complicated by gene-environment interactions. Although genomic, transcriptomic, proteomic analyses have been performed to investigate the pathogenesis of psoriasis, role metabolites in particularly lipids, remains unclear. Lipids not only comprise bulk cellular membrane bilayers but also regulate variety biological processes such as cell proliferation, apoptosis, immunity, angiogenesis, inflammation. In this study, an...
Abstract Controlling vertical phase separation of the active layer to enable efficient exciton dissociation and charge carrier transport is crucial boost power conversion efficiencies (PCEs) pseudoplanar heterojunction (PPHJ) organic solar cells (OSCs). However, how optimize PPHJ OSCs via molecule design rarely reported yet. Herein, ternary polymerization strategy employed develop a series polymer donors, DL1‐DL4, regulate their solubility, molecular aggregation, orientation, miscibility,...
To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma.Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray® Human MicroRNA Panel. profiles glioblastoma patients controls created three discovery cohorts confirmed in two validation cohorts.miRNA clinical correlated with those found tissues. Comparison of between non-brain yielded "signature" consisting nine miRNAs. The volume (p=0.008)....
Abstract Sinocyclocheilus represents a rare, freshwater teleost genus endemic to China that comprises the river-dwelling surface fish and cave-dwelling cavefish. Using combinatorial approach of quantitative lipidomics mass-spectrometry imaging (MSI), we demonstrated neural compartmentalization lipid distribution metabolism is associated with evolution troglomorphic traits in Sinocyclocheilus. Attenuated docosahexaenoic acid (DHA) biosynthesis via Δ4 desaturase pathway led reductions...
Specific drug delivery to metastatic tumors remains a great challenge for antimetastasis therapy. We herein report bioengineered macrophage-based system (LD-MDS) that can be preferentially delivered lung metastases and intelligently transformed into nanovesicles secondary LD-MDS was prepared by anchoring legumain-specific propeptide of melittin (legM) cytotoxic soravtansine (DM4) prodrug onto the membrane living macrophages. is responsively activated legumain protease converted DM4-loaded...
Spontaneous mutations introduce uncertainty into coronavirus disease 2019 (COVID-19) control procedures and vaccine development. Here, we perform a spatiotemporal analysis on intra-host single-nucleotide variants (iSNVs) in 402 clinical samples from 170 affected individuals, which reveals an increase genetic diversity over time after symptom onset individuals. Nonsynonymous are overrepresented the pool of iSNVs but underrepresented at polymorphism (SNP) level, suggesting two-step fitness...
Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation long-term culture of bovine trophoblast (TSCs). Bovine TSCs retain developmental potency to differentiate into mature exhibit transcriptomic epigenetic (chromatin accessibility DNA methylome) features characteristic trophectoderm from...
Arh1p is an essential mitochondrial protein of yeast with reductase activity. Here we show that this involved in iron metabolism. A strain was constructed which the open reading frame placed under control a galactose-regulated promoter. Protein expression induced by galactose and repressed to undetectable levels absence galactose, although cells grew quite well inducer. Under noninducing conditions, cellular uptake dysregulated, exhibiting failure repress response medium iron. Iron...
Mechanistic basis governing the extreme longevity and developmental quiescence of dauer juvenile, a "non-ageing" variant Caenorhabditis elegans , has remained largely obscure. Using lipidomic approach comprising multiple reaction monitoring transitions specific to distinct fatty acyl moieties, we demonstrated that in comparison other stages, membrane phospholipids larva contain unique enrichment polyunsaturated acids (PUFAs). Esterified PUFAs exhibited temporal accumulation throughout course...
Coronavirus 2019 (COVID-19) is a complex disease that affects billions of people worldwide. Currently, effective etiological treatment COVID-19 still lacking; also causes damages to various organs therapeutics and mortality the patients. Surveillance responses organ injury assessment patients are high clinical value. In this study, we investigated characteristic fragmentation patterns explored potential in tissue plasma cell-free DNA Through recruitment 37 patients, 32 controls analysis 208...
A fundamental question in developmental neurobiology is how a common neurotransmitter specified different neuronal types?. We describe cell-specific regulation of the serotonergic phenotype by C. elegans POU-transcription factor UNC-86. show that unc-86 regulates particular aspects terminal identity four classes neurons, but development ADF neurons regulated an UNC-86-independent program. In NSM role confined late differentiation; are generated do not express genes necessary for...
Lipoprotein-associated phospholipase A2 (Lp-PLA2) is thought to play modulatory roles in the development of atherosclerosis. Here we evaluated effects a specific lp-PLA2 inhibitor on atherosclerosis ApoE-deficient mice and its associated mechanisms.ApoE-deficient fed an atherogenic high-fat diet for 17 weeks were divided into two groups. One group was administered inhibitor, darapladib (50 mg/kg/day; p.o.) daily 6 weeks, while control saline. We observed no differences body weight serum...
The classical form of α1-antitrypsin deficiency (ATD) is associated with hepatic fibrosis and hepatocellular carcinoma. It caused by the proteotoxic effect a mutant secretory protein that aberrantly accumulates in endoplasmic reticulum liver cells. Recently we developed model this C. Elegans adapted it for high-content drug screening using an automated, image-based array scanning. Screening Library Pharmacologically Active Compounds identified fluphenazine (Flu) among several other compounds...
The accumulation of serpin oligomers and polymers within the endoplasmic reticulum (ER) causes cellular injury in patients with classical form α1-antitrypsin deficiency (ATD). To better understand molecular genetic aspects this disorder, we generated transgenic C. elegans strains expressing either wild-type (ATM) or Z mutant (ATZ) human fused to GFP. Animals secreted ATM, but retained polymerized ATZ dilated ER cisternae. These latter animals also showed slow growth, smaller brood sizes...
Actinobacteria is a ubiquitous major group in coral holobiont. The diversity and spatial temporal distribution of actinobacteria have been rarely documented. In this study, associated with mucus, tissue skeleton Porites lutea the surrounding seawater were examined every 3 months for 1 year on Luhuitou fringing reef. population structures P. lutea-associated analyzed using phylogenetic analysis 16S rRNA gene clone libraries, which demonstrated highly diverse profiles lutea. A total 25...
Epigallocatechin-3-gallate (EGCG), a main active catechin in green tea, was reported to attenuate renal injury and hypertension. However, its effects on salt-induced hypertension remain unclear. In the present study, we explored damage Dahl rats with salt-sensitive We found that EGCG could lower blood pressure after 6 weeks of oral administration, reduce 24 h urine protein levels decrease creatinine clearance, fibrosis, indicating it by protecting against damage. Furthermore, studied...
Inflammation plays an important role in the progression of sporadic aortic dissection (AD). Immune cells, especially macrophages, infiltrate aorta and secrete inflammatory cytokines matrix metalloproteinases to cause degradation extracellular matrix, thereby contributing pathogenesis AD. However, cellular heterogeneity within these immune cells has not been fully characterized.We used single-cell RNA sequencing profile transcriptomes all AD tissue normal aorta. Using magnetic-activated cell...
Hypoxic exposure makes plateau migrators susceptible to high altitude polycythemia (HAPC). Astragalus membranaceus (AM) is an edible and medicinal plant with remarkable immunomodulatory activities. The purpose of this study was discover if AM could be a candidate for the prevention HAPC its mechanism. Here, network pharmacology applied screen active compounds, key targets, enriched pathways in treatment HAPC. Molecular docking evaluated affinity between compounds core targets. Subsequently,...