A5031748572- Sphingolipid Metabolism and Signaling
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Analytical Chemistry and Chromatography
- Nanocluster Synthesis and Applications
- Lipid metabolism and biosynthesis
- Cancer, Lipids, and Metabolism
- Molecular Sensors and Ion Detection
- Protein Kinase Regulation and GTPase Signaling
- Caveolin-1 and cellular processes
- Liver Disease Diagnosis and Treatment
- Luminescence and Fluorescent Materials
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- RNA modifications and cancer
- Crystallization and Solubility Studies
- Electrochemical sensors and biosensors
- Bone health and treatments
- X-ray Diffraction in Crystallography
- Advanced Proteomics Techniques and Applications
- Analytical chemistry methods development
- Advanced oxidation water treatment
Beijing National Laboratory for Molecular Sciences
2016-2025
University of Chinese Academy of Sciences
2016-2025
Chinese Academy of Sciences
2016-2025
Graduate School USA
2025
Harbin Institute of Technology
2024
Institute of Chemistry
2003-2021
Shanghai University
2021
Weatherford College
2015
Indiana University – Purdue University Indianapolis
2006-2013
Indiana University School of Medicine
2007-2013
Plasma levels of lysophospholipids were evaluated as potential biomarkers for colorectal cancer (CRC), where a highly reliable and minimally invasive blood test is lacking.Patients with CRC (n = 133) control subjects 125) recruited through the Cleveland Clinic. Preoperative plasma samples analyzed lysophospholipid using liquid chromatography mass spectrometry in blinded fashion. Participants randomly divided 2:1 ratio into "training set" (TS) "validation (VS). Logistic regression models used...
Abstract Lysophosphatidic acid (LPA) is both a potential marker and therapeutic target for ovarian cancer. It critical to identify the sources of elevated LPA levels in ascites blood patients with We show here that human peritoneal mesothelial cells constitutively produce LPA, which accounts significant portion chemotactic activity conditioned medium from cancer cells. Both production by can be blocked HELSS [an inhibitor calcium-independent phospholipase A2 (iPLA2)] AACOCF3 cytosolic PLA2...
Rheumatoid arthritis is a destructive arthropathy characterized by chronic synovial inflammation that imposes substantial socioeconomic burden. Under the influence of proinflammatory milieu, fibroblasts (SFs), main effector cells in disease pathogenesis, become activated and hyperplastic, releasing factors tissue-remodeling enzymes. This study shows arthritic SFs from human patients animal models express significant quantities autotaxin (ATX; ENPP2), lysophospholipase D catalyzes conversion...
Autotaxin (ATX) is an extracellular lysophospholipase D that generates lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC). Both ATX and LPA have been shown to be involved in many cancers. However, the functional role of regulation expression human hepatocellular carcinoma (HCC) remain elusive. In this study, was evaluated tissues 38 HCC 10 normal control subjects. detected mainly tumor cells within tissue sections its over-expression specifically correlated with inflammation...
Increased epithelial cell apoptosis in response to lung injury has been implicated the development of idiopathic pulmonary fibrosis (IPF), but molecular pathways promoting this disease have yet be fully identified. Lysophosphatidic acid (LPA), which we previously demonstrated mediate bleomycin injury-induced fibroblast recruitment and vascular leak mice patients with IPF, is an important regulator survival many types. We now show that LPA signaling through its receptor LPA(1) promotes...
Ferroptosis, an iron-dependent regulated cell death process driven by excessive lipid peroxides, can enhance cancer vulnerability to chemotherapy, targeted therapy and immunotherapy. As essential upstream for ferroptosis activation, peroxidation of biological membranes is expected be primarily induced intrabilayer reactive oxygen species (ROS), indicating a promising strategy initiate improving the local content diffusion-limited ROS in bilayer. Herein, liposomes embedded with PEG-coated 3...
Abstract Sinocyclocheilus represents a rare, freshwater teleost genus endemic to China that comprises the river-dwelling surface fish and cave-dwelling cavefish. Using combinatorial approach of quantitative lipidomics mass-spectrometry imaging (MSI), we demonstrated neural compartmentalization lipid distribution metabolism is associated with evolution troglomorphic traits in Sinocyclocheilus. Attenuated docosahexaenoic acid (DHA) biosynthesis via Δ4 desaturase pathway led reductions...
Abstract Early diagnosis and intervention of Alzheimer’s disease (AD) are particularly important to delay the pathological progression. Although fluorescent probes have been widely employed for investigating diagnosing AD, their biological applications significantly restricted due low penetration ability blood–brain barrier (BBB) in vivo. In this study, we reported first Golgi-targeted two-photon (TP) probe, DCM-DH, detecting viscosity Golgi apparatus. The probe was rationally designed...
Calcium-independent phospholipase A(2) (iPLA(2)) plays a pivotal role in phospholipid remodeling and many other biological processes, including inflammation cancer development. iPLA(2) can be activated by caspase-3 via proteolytic process apoptotic cells. In this study we identify novel signaling functional loops of activation leading to migration non-apoptotic human ovarian The extracellular matrix protein, laminin-10/11, but not collagen I, induces integrin- caspase-3-dependent cleavage...
Ovarian cancer G protein-coupled receptor 1 (OGR1) has been shown to be a proton sensing in vitro. We have that OGR1 functions as tumor metastasis suppressor gene when it is over-expressed human prostate cells vivo. To examine the physiological of OGR1, we generated conditional deficient mice by homologous recombination. were viable and upon gross-inspection appeared normal. Consistent with vitro studies showing involved osteoclastogenesis, reduced osteoclasts detected mice. A pH-dependent...
Background: The aim of this study was to characterize the disorder lipid metabolism in hepatocellular carcinoma (HCC). HCC is a worldwide disease. research into very limited. Study liver cancer tissue may have potential provide new insight mechanisms. Methods: A lipidomics based on Ultra high performance liquid chromatography-electronic spray ionization-QTOF mass spectrometer (UPLC-ESI-QTOF MS) and Matrix assisted laser desorption ionization-fourier transform ion cyclotron resonance...
Abstract MALDI MSI has been recently applied as an innovative tool for detection of molecular distribution within a specific tissue. requires deposition organic compound, known matrix, on the tissue interest to assist analyte desorption and ionization, in which matrix crystal homogeneity size greatly influence imaging reproducibility spatial resolution MSI. In this work, homemade electrospray device was developed The could be used achieve 1 μm homogeneous crystals analysis. Moreover, it...
Quantitative measurements of sex difference in vesicle chemistry (i.e., chemical storage and release) at the single-vesicle level are essential to understand differences cognitive behaviors; however, such very challenging conventional analytical methods. By using electrochemistry, we find duration single exocytotic events chromaffin cells prepared from male rats is statistically longer than that female rats, leading more neurotransmitter released group. Further analysis reveals a higher...
Monounsaturated fatty acids (MUFA) are an important class of nutrients and involved in lipid metabolism. The positions the C=C bond cis-trans isomerism have a significant influence on their physiological activity. However, simultaneously detecting these two structural properties has been challenging due to multiple isomers MUFA. In this study, we developed method that N-aminophthalimide (PhthNH2) derivatization MUFA, followed by analysis using ultrahigh-performance liquid...
Ethanol treatment of cultured hepatoma cells and mice inhibited the activity AMP-activated protein kinase (AMPK). This study shows that inhibitory effect ethanol on AMPK phosphorylation is exerted through inhibition upstream kinases activation phosphatase 2A (PP2A).Inhibition by palmitate was attributed to ceramide-dependent PP2A activation. We hypothesized mediated partly generation ceramide. The inhibitors ceramide synthesis phosphorylation, levels, were assessed in rat (H4IIEC3). hepatic...
Our previous data showed the inhibitory effect of ethanol on AMP-activated protein kinase phosphorylation, which appears to be mediated, in part, through increased levels hepatic ceramide and activation phosphatase 2A (Liangpunsakul S, Sozio MS, Shin E, Zhao Z, Xu Y, Ross RA, Zeng Crabb DW. Am J Physiol Gastrointest Liver 298: G1004-G1012, 2010). The phosphorylation was reversed by imipramine, suggesting that generation via acid sphingomyelinase (ASMase) is stimulated ethanol. In this study,...
Fatty acid synthase (FASN) is a key enzyme in the synthesis of palmitate, precursor major nutritional, energetic, and signaling lipids. FASN expression upregulated many human cancers appears to be important for cancer cell survival. Overexpression has also been found associate with poor prognosis higher risk recurrence cancers. Indeed, elevated shown contribute drug resistance. However, mechanism FASN-mediated resistance currently unknown. In this study, we show that overexpression causes...