A5029136370- Bioinformatics and Genomic Networks
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Computational Drug Discovery Methods
- Liver physiology and pathology
- RNA modifications and cancer
- Gene Regulatory Network Analysis
- Extracellular vesicles in disease
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Cancer-related gene regulation
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- interferon and immune responses
- Alzheimer's disease research and treatments
- Pluripotent Stem Cells Research
- Gene expression and cancer classification
- Liver Diseases and Immunity
- Kruppel-like factors research
- Neuroinflammation and Neurodegeneration Mechanisms
Second Military Medical University
2016-2024
Eastern Hepatobiliary Surgery Hospital
2016-2024
Shanghai Cancer Institute
2016-2022
Beijing Radiation Center
2011-2015
Abstract COVID-19, caused by SARS-CoV-2, has recently affected over 1,200,000 people and killed more than 60,000. The key immune cell subsets change their states during the course of COVID-19 remain unclear. We sought to comprehensively characterize transcriptional changes in peripheral blood mononuclear cells recovery stage single-cell RNA sequencing technique. It was found that T decreased remarkably, whereas monocytes increased patients early (ERS) COVID-19. There an ratio classical CD14...
Background and aims Liver fibrosis is a wound-healing response that disrupts the liver architecture function by replacing functional parenchyma with scar tissue. Recent progress has advanced our knowledge of this scarring process, but detailed mechanism far from clear. Methods The fibrotic specimens patients HLF (hepatic leukemia factor) PB/PB mice were used to assess expression role in fibrosis. Primary murine hepatic stellate cells (HSCs) human HSC line Lx2 investigate impact on activation...
Hepatocellular carcinoma (HCC) is one of the fastest-rising causes cancer-related death worldwide, but its deficiency specific biomarkers and therapeutic targets in early stages lead to severe inadequacy diagnosis treatment HCC. Covalently closed circular RNA (circRNA), which was once considered an aberrant splicing by-product, now drawing new interest cancer research because remarkable functionality. Beneath surface dominant functional proteins events, a hidden circRNA-centric noncoding...
Abstract Induction of tumor vascular normalization is a crucial measure to enhance immunotherapy efficacy. cGAS-STING pathway vital for anti-tumor immunity, but its role in vasculature unclear. Herein, using preclinical liver cancer models Cgas / Sting -deficient male mice, we report that the interdependence between cGAS and host STING mediates immune response. Mechanistically, TET2 mediated IL-2/STAT5A signaling epigenetically upregulates expression produces cGAMP. Subsequently, cGAMP...
Background and aims The unique expression pattern makes oncofetal proteins ideal diagnostic biomarkers therapeutic targets in cancer. However, few have been identified entered clinical practice. Methods Fetal liver, adult liver hepatocellular carcinoma (HCC) tissues were employed to assess the of hepatic leukaemia factor (HLF). impact HLF on HCC onset progression was investigated both vivo vitro. association between patient prognosis determined cohorts. correlation sorafenib benefits further...
Abstract Hepatocyte nuclear factor 3γ (HNF3γ) is a hepatocyte factor, but its role and clinical significance in hepatocellular carcinoma (HCC) remain unclear. Herein, we report that HNF3γ expression downregulated patient HCC inversely correlated with malignancy survival. Moreover, our data suggested the reduction could be mediated by METTL14-dependent m6A methylation of mRNA. was increased during hepatic differentiation decreased dedifferentiated cells. Interestingly, delivery promoted not...
Gallbladder carcinoma (GBC) is the most common biliary tract malignancy with lowest survival rate, primarily arising from chronic inflammation. To better characterize progression inflammation to cancer metastasis, we performed single-cell RNA sequencing across samples of 6 cholecystitis, 12 treatment-naive GBCs, and matched metastases. Benign epithelial cells inflamed gallbladders displayed resting, immune-regulating, gastrointestinal metaplastic phenotypes. A small amount PLA2G2A+ copy...
Abstract COVID-19, caused by SARS-CoV-2, has recently affected over 300,000 people and killed more than 10,000. The manner in which the key immune cell subsets change their states during course of COVID-19 remain unclear. Here, we applied single-cell technology to comprehensively characterize transcriptional changes peripheral blood mononuclear cells recovery stage COVID-19. Compared with healthy controls, patients early (ERS) T decreased remarkably, whereas monocytes increased. A detailed...
Harboring the behavioral and histopathological signatures of Alzheimer's disease (AD), senescence accelerated mouse-prone 8 (SAMP8) mice are currently considered a robust model for studying AD. However, underlying mechanisms, prioritized pathways genes in SAMP8 linked to AD remain unclear. In this study, we provide biological interpretation molecular underpinnings mice. Our results were derived from differentially expressed hippocampus cerebral cortex compared age-matched SAMR1 at 2, 6, 12...
Precancerous lesion, a well-established histopathologically premalignant tissue with the highest risk for tumourigenesis, develops preferentially from activation of DNA damage checkpoint and persistent inflammation. However, little is known about mechanisms by which precancerous lesions are initiated their physiological significance.Laser capture microdissection was used to acquire matched normal liver, lesion tumour tissues. miR-484(-/-), Ifnar1(-/-) Tgfbr2(△hep) mice were employed...
Abstract Coronavirus disease 2019 (COVID-19), driven by SARS-CoV-2, is a severe infectious that has become global health threat. Vaccines are among the most effective public tools for combating COVID-19. Immune status critical evaluating safety and response to vaccine, however, evolution of immune during immunization remains poorly understood. Single-cell RNA sequencing (scRNA-seq) represents powerful tool dissecting multicellular behavior discovering therapeutic antibodies. Herein,...
Despite the efficacy demonstrated by immunotherapy recently, liver cancer still remains one of deadliest cancers, mainly due to heterogeneity this disease. Continuous exploration new therapeutics is therefore necessary. Chemical-induced cell differentiation can serve as a promising approach, with its ability consistently remodel gene expression profile and alter fate. Inspired advances in stem reprogramming field, here it reported that small molecule cocktail (SMC) consisted of: SB431542...
Comparisons of gene expression signatures provide a way to explore functional connections among biological events in global aspects cell response. GeneExpressionSignature is an R package developed for the large-scale analysis signatures. The implements two rank-merging algorithms and similarity-scoring algorithms. functions flexible solution signature-based studies hold great potential biomedical research applications, such as drug repurposing. released under GPL v2 within Bioconductor...
Cationic liposomes (CLs) can be used as non-viral vectors in gene transfer and drug delivery. However, the underlying molecular mechanism of its cytotoxicity has not been well elucidated yet. We herein report a systems biology approach based on whole-transcriptome sequencing coupled with computational method to identify predominant genes pathways involved CLs HepG2 cell line. Firstly, we validated concentration-dependent an IC50 120 μg/ml exposed for 24 h. Subsequently, 220 (77 up- 143...
There are currently no approved effective therapies for Alzheimer’s disease (AD). AD is a classic, multifactorial, complex syndrome. Thus, polypharmacological or multitargeted approach to might provide better therapeutic benefits than monotherapies. However, it remains elusive which biological processes and biomolecules involved in the pathophysiologic of would constitute good targets therapy. This study proposes that co-module, consisting processes, cellular pathways nodes, molecular...
Abstract It can be difficult for biomedical researchers to understand complex molecular networks due their unfamiliarity with the mathematical concepts employed. To represent clear meanings and familiar forms researchers, we introduce a knowledge-based computational framework decipher by making systematic comparisons well-studied “basic networks”. A network is characterized as spectrum-like vector called “network fingerprint”, which contains similarities basic networks. This multidimensional...
Human miRNAs have recently been found to important roles in viral replication. Understanding the patterns and details of human miRNA interactions during virus-host may help uncover novel antiviral therapies. Based on abundance knowledge available regarding protein-protein (PPI), protein interactions, experimentally validated miRNA-target pairs transcriptional regulation miRNAs, it is possible explore complex regulatory network that exists between proteins at system level.By integrating...
Polyene phosphatidylcholine (PPC) is a widely used hepatoprotective drug. We aim to explore the effectiveness of PPC in patients with liver diseases based on real-world research, and compare other drugs. This was 'three-phase' retrospective study, including descriptive self-control case specific-disease cohort study. A total 14,800 hospitalized were enrolled phase I from 1 January 2015 2020, which 793 using alone included for II III. The major measurement analysis ALT level its changes....