A5051811672- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Cellular transport and secretion
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
- Genetic Syndromes and Imprinting
- Monoclonal and Polyclonal Antibodies Research
- RNA regulation and disease
- Liver Disease and Transplantation
- Adenosine and Purinergic Signaling
- Immune cells in cancer
- Genetics and Neurodevelopmental Disorders
- Acute Kidney Injury Research
- Immune Response and Inflammation
- Microbial Natural Products and Biosynthesis
- Hepatocellular Carcinoma Treatment and Prognosis
- Liver physiology and pathology
- Renal and Vascular Pathologies
- HER2/EGFR in Cancer Research
- Pain Mechanisms and Treatments
- Neuroscience of respiration and sleep
- Renal and related cancers
- Kruppel-like factors research
General Hospital of Guangzhou Military Command
2017-2025
Chinese PLA General Hospital
2018-2024
Chinese People's Liberation Army
2021-2024
State Key Laboratory of Kidney Diseases
2018-2024
Brown University
2011-2024
Third Affiliated Hospital of Southern Medical University
2021-2024
Bradley Hospital
2016-2023
Western General Hospital
2023
Providence College
2023
Allen Institute for Brain Science
2021-2023
The triple-negative breast cancer (TNBC) microenvironment makes a feature of aberrant vasculature, high interstitial pressure, and compact extracellular matrix, which combine to reduce the delivery penetration therapeutic agents, bringing about incomplete elimination cells. Herein, employing tumor strategy size-shrinkage combined with ligand modification, we constructed photothermal nanocluster for cascaded deep in parenchyma efficient eradication TNBC In our approach, agent indocyanine...
Significance We report autosomal recessive mutations in the enzyme glutamate pyruvate transaminase 2 (GPT2) a neurological syndrome involving intellectual disability, reduced brain growth, and progressive motor symptoms. show that inactivate enzyme. GPT2 catalyzes reversible addition of an amino group from to pyruvate, yielding alanine α-ketoglutarate. The gene demonstrates expression postnatally, protein localizes mitochondria. As humans, Gpt2 -null mice exhibit growth. Furthermore, mutant...
Abstract We aimed to investigate the role of renal pericyte pyruvate kinase M2 (PKM2) in progression acute kidney injury (AKI) chronic disease (CKD). The PKM2 pericyte‐myofibroblast transdifferentiation was investigated an AKI‐CKD mouse model. Platelet growth factor receptor beta (PDGFRβ)‐iCreERT2; tdTomato mice were used for tracing. Western blotting and immunofluorescence staining examine protein expression. An 5‐ethynyl‐2′‐deoxyuridine assay measure proliferation. A scratch cell migration...
Precancerous lesion, a well-established histopathologically premalignant tissue with the highest risk for tumourigenesis, develops preferentially from activation of DNA damage checkpoint and persistent inflammation. However, little is known about mechanisms by which precancerous lesions are initiated their physiological significance.Laser capture microdissection was used to acquire matched normal liver, lesion tumour tissues. miR-484(-/-), Ifnar1(-/-) Tgfbr2(△hep) mice were employed...
Neurons from patient-derived iPSCs demonstrate mutation-specific responses to potential therapeutic strategies for Christianson syndrome.
Ischemia-reperfusion injury (IRI) is a major cause of chronic renal fibrosis. Currently, numerous therapies have shown minimal effect on the blockade fibrosis progression. Here, therapeutic potential peptide-based TGF-β1 inhibitor P144 in IRI-induced and underlying mechanism were analyzed.The unilateral ischemia-reperfusion with contralateral nephrectomy model was established, administered intravenously 1d/14d after onset IRI. The histopathology immunofluorescence staining used to detect...
It is urgent that the means to improve liver regeneration (LR) be found, while mitigating concurrent risk of hepatocarcinogenesis (HCG). Nuclear receptor corepressor 1 (NCoR1) a co-repressor nuclear receptors, which regulates expression level metabolic genes; however, little known about its potential contribution for LR and HCG. Here, we found liver-specific NCoR1 knockout in mice (NCoR1Δhep ) dramatically enhances after partial hepatectomy and, surprisingly, blocks process...
Loss-of-function mutations in the X-linked endosomal Na+/H+ exchanger 6 (NHE6) cause Christianson syndrome males. involves endosome dysfunction leading to early cerebellar degeneration, as well later-onset cortical and subcortical neurodegeneration, potentially including tau deposition reported post-mortem studies. In addition, there is evidence of modulation amyloid-β levels experimental models wherein NHE6 expression was targeted. We have recently shown that loss causes defects maturation...
Chronic liver injury leads to progressive fibrosis and ultimately cirrhosis, a major cause of morbidity mortality worldwide. However, there are currently no effective anti-fibrotic therapies available, especially for late-stage patients, which is partly attributed the knowledge gap regarding cell heterogeneity cell-specific responses in different stages. To reveal multicellular networks regulating mammalian from mild severe phenotypes, we generated single-nucleus transcriptomic atlas...
The growing world population and increasing life expectancy are driving the need to improve quality of blood transfusion, organ transplantation, preservation. Here, ability red cells (RBCs) for normothermic machine perfusion, a biocompatible silicification approach termed "shielding-augmenting RBC-in-nanoscale amorphous silica (SARNAS)" has been developed. key RBC surface engineering structure augmentation is precise control hydrolysis form silicic acid realize stabilization within conformal...
Apoptosis is of considerable importance in the pathogenesis emphysema, and recent studies show that endoplasmic reticulum (ER) stress involved emphysema. In our research, we investigated role protein kinase RNA (PKR)-like ER (PERK)/ eukaryotic initiation factor 2 alpha (eIF2α) pathway, CCAAT enhancer-binding protein-homologous (CHOP) expression, caspase-12 activation apoptosis emphysema results from cigarette smoke (CS) exposure. Expression phosphorylated-PERK (p-PERK), phospholated-eIF2α...
Abstract Christianson syndrome (CS) is an X-linked disorder resulting from loss-of-function mutations in SLC9A6 , which encodes the endosomal Na + /H exchanger 6 (NHE6). Symptoms include early developmental delay, seizures, intellectual disability, nonverbal status, autistic features, postnatal microcephaly, and progressive ataxia. Neuronal development impaired CS, involving defects neuronal arborization synaptogenesis, likely underlying diminished brain growth postnatally. In addition to...
Burkholderiales are an emerging source of bioactive secondary metabolites and have the potential to be a robust chassis for from Gram-negative bacteria. However, only few constitutive promoters can utilized in Burkholderiales. Herein, we described screening strong strain DSM 7029, 37 identified transcriptome sequencing were cloned characterized using firefly luciferase reporter further verified by qPCR analysis. These then used drive complex 56-kb epothilone BGC myxobacterium 23-kb rhizomide...
Abstract Objectives We aimed to investigate the underlying mechanism of endothelial cells (ECs) proliferation in anti‐Thy‐1 nephritis. Materials and methods established nephritis co‐culture system explore ECs vivo vitro. EdU assay kit was used for measuring cell proliferation. Immunohistochemical staining immunofluorescence were detect protein expression. ELISA measure concentration serum medium. RT‐qPCR Western blot qualify mRNA siRNA knock down specific Results In nephritis, associated...
COPD is a global disease characterized by chronic bronchitis and obstructive emphysema. Its pathogenesis not fully understood. This study aimed to use proteomics provide new insights into the mechanisms of COPD.Protein lysates were prepared from lung tissue samples harvested never-smokers, non-COPD smokers smokers, analysed using 2-dimensional gel electrophoresis. Differentially expressed proteins identified mass spectrometry. The differential expression heat shock protein 27 (Hsp27)...
Objective . There is some evidence implicating receptor for advanced glycation end products (RAGE) signaling in the pathogenesis of neuropathic pain (NP). The objective was to investigate whether RAGE dorsal root ganglion (DRG) might contribute NP following peripheral nerve injury. Design Experimental study before and after spinal ligation (SNL) surgery. Setting Caged a controlled environment. Subjects Male Sprague-Dawley rats. Methods A SNL rat model used. Mechanical hyperalgesia measured...
We cloned 10 full-length variants of PIIH, the gene for I(h) from spiny lobster, Panulirus interruptus, using reverse transcription-PCR (RT-PCR) and rapid amplification cDNA ends (RACE). This shows a significant amount alternative splicing in S3-S4 S4-S5 linkers, P-loop entire S6 transmembrane domain, cyclic nucleotide binding domain (CNBD), near 3' end gene. Functional expression seven splice Xenopus oocytes generated slowly activating hyperpolarization-activated inward currents, which were...
Cholangiocarcinoma (CCA) is an aggressive malignant tumor and the prognosis of patients with advanced stage disease remains poor. Therefore, identification novel treatment agents for CCA required. In present study, biological effects diabetes therapeutic agent, phenformin, in cell lines was investigated. Cell Counting Kit‑8 viability, cellular clone formation subcutaneous assays were performed, which revealed that phenformin inhibited proliferation growth both vitro vivo. addition, induced...