A5012635551- Adenosine and Purinergic Signaling
- Neuroscience of respiration and sleep
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Pain Mechanisms and Treatments
- Neuroendocrine regulation and behavior
- Sleep and Wakefulness Research
- Neuroendocrine Tumor Research Advances
- Genetics and Neurodevelopmental Disorders
- Neuroscience and Neuropharmacology Research
- Adolescent and Pediatric Healthcare
- Barrier Structure and Function Studies
- Circadian rhythm and melatonin
- MicroRNA in disease regulation
- Ion channel regulation and function
- Hydrogen's biological and therapeutic effects
- Nitric Oxide and Endothelin Effects
- RNA Research and Splicing
- Gastroesophageal reflux and treatments
- Vagus Nerve Stimulation Research
- S100 Proteins and Annexins
- Immune cells in cancer
- Restless Legs Syndrome Research
- Neurological Complications and Syndromes
Second Military Medical University
2013-2022
Zhejiang University
2007
Roland Hill (United Kingdom)
1998-2005
The Royal Free Hospital
1998-2005
University College London
1998-2005
Roche (Switzerland)
2003
The regulation of inflammation is pivotal for preventing the development or reoccurrence multiple sclerosis (MS). A biased ratio high-M1 versus low-M2 polarized microglia a major pathological feature MS Here, using microarray screening, we identify long noncoding RNA (lncRNA) GAS5 as an epigenetic regulator microglial polarization. Gain- and loss-of-function studies reveal that suppresses M2 Interference with in transplanted attenuates progression experimental autoimmune encephalomyelitis...
In mammals, the ventilatory response to decreased oxygen tension in arterial blood is initiated by excitation of specialized O2-sensitive chemoreceptor cells carotid body that release neurotransmitters activate endings sinus nerve afferent fibers. We investigated role ATP acting via ionotropic P2X receptors function and hypoxia mice. Mice deficient P2X2 receptor subunit showed a markedly attenuated hypoxia, whereas P2X3-deficient mice was comparable with seen wild-type controls. P2X3...
Major depressive disorder ranks as a major burden of disease worldwide, yet the current antidepressant medications are limited by frequent non-responsiveness and significant side effects. The lateral septum (LS) is thought to control depression, however, cellular circuit substrates largely unknown. Here, we identified subpopulation LS GABAergic adenosine A
P2X receptors have been suggested to play a role in the transduction of sensory signals such as pain and sound. In present study, polyclonal antibodies against P2X1 P2X6 were used localize circumvallate fungiform papillae rats. Nerve fibres innervating taste buds stained intensely with P2X3 receptor antibodies. receptor-positive nerves observed intra- subgemmal regions. The nerve also P2X2 antibodies, but intensity was much lower. distribution immunoreactivity overlaps that P2X3. These...
Background The pathology of Parkinson's disease (PD) is characterized by the degeneration nigrostriatal dopaminergic pathway, as well formation intraneuronal inclusions known Lewy bodies and neurites in substantia nigra. Accumulations nitrated α-synuclein are demonstrated signature disease. However, whether nitration relevant to pathogenesis PD unknown. Methodology/Principal Findings In this study, effect (DA) neurons was determined delivering recombinant TAT-α-synuclein intracellular. We...
The development of hippocampal circuitry depends on the proper assembly correctly specified and fully differentiated neurons. Little is known about factors that control specification. Here, we show zinc finger protein Zbtb20 essential for specification CA1 field identity. We found expression was initially activated in anlage at onset corticogenesis, persisted immature Targeted deletion mice did not compromise progenitor proliferation adjacent transitional ventricular zone, but led to...
The major challenge for progressive multiple sclerosis therapy is the promotion of remyelination from inflammation-induced demyelination. A switch an M1- to M2-dominant polarization microglia critical in these repair processes. In this study, we identified homeobox gene msh-like homeobox-3 ( Msx3 ) as a new pivotal regulator microglial polarization. MSX3 was induced during M2 and repressed M1 cells. expression dynamically regulated experimental autoimmune encephalomyelitis (EAE), which...
Tanshinone IIA (Tan IIA), one of the major active constituents medicinal herb Salvia miltiorrhiza, has been reported to possess neuroprotective effects against pathological features Alzheimer's disease (AD), but molecular mechanism underlying this effect remains unclear. To examine Tan on AD, as well mechanisms, in vivo animal experiments and vitro biology investigations were employed present study. Firstly, a rat model AD was successfully established by direct injection amyloid beta protein...
Abstract Objective To assess the effect of serine protein inhibitor A3N (serpinA3N) in ischemic stroke and to explore its mechanism action. Methods Mouse model was induced by transient middle cerebral artery occlusion followed reperfusion. The expression pattern serpinA3N assessed using immunofluorescence, Western blot analysis, real‐time quantitative PCR. An adeno‐associated virus (AAV) recombinant were administered. Additionally, co‐immunoprecipitation‐mass spectrometry immunofluorescence...
Blood-brain barrier (BBB) impairment is an early prevalent feature of multiple sclerosis (MS), and remains vital for MS progression. Microglial activation precedes BBB disruption cellular infiltrates in the brain patients. However, little known about function microglia impairment. Here, acts as important modulator integrity inflammatory demyelination. depletion profoundly ameliorates experimental autoimmune encephalomyelitis (EAE). Specifically, miR-126a-5p positively correlated with four...
Abstract We have used single‐ and double‐labeling immunfluorescence reverse transcription‐polymerase chain reaction (RT‐PCR) methods to examine expression of P2X receptor subtypes on microglial cells brain in late embryonic postnatal rat, the N9 cell line primary cultured cells. 1 , 4 7 receptors were shown from day 16. Almost all that positive for marker ED1, expressed receptors, whereas only about 30% with ED1‐immunoreactivity found express receptor. Positive localized mainly white matter...
P2X receptors on dorsal root ganglion (DRG) neurons have been strongly implicated in pathological nociception after peripheral nerve injuries or inflammation. However, nothing is known of a role for purinergic neuropathic pain produced by chronic compression DRG (CCD) - an injury that may accompany intraforaminal stenosis, laterally herniated disc other disorders the spine leading to radicular pain. In rat model compression, hyperexcitable retain functioning axonal connections with their...
Intronic polymorphisms of the GABAA receptor β2 subunit gene (GABRB2) under adaptive evolution were associated with schizophrenia and reduced expression, especially long isoform which differs in electrophysiological properties from short isoform. The present study was directed to examining dosage effects Gabrb2 knockout mice both heterozygous (HT) homozygous (KO) genotypes respect possible schizophrenia-like comorbid phenotypes. KO mice, HT a lesser extent, found display prepulse inhibition...
Background Reactive oxygen and nitrogen species are key molecules that mediate neuropathic pain. Although hydrogen is an established antioxidant, its effect on chronic pain has not been characterized. This study was to investigate the efficacy mechanisms of hydrogen-rich normal saline induced analgesia. Methodology/Principal findings In a rat model by L5 spinal nerve ligation (L5 SNL), intrathecal injection relieved SNL-induced mechanical allodynia thermal hyperalgesia. Importantly, repeated...
Spinal cord injury (SCI) can induce excessive astrocyte activation. Hydrogen has been deemed as a novel antioxidant. We investigated whether molecular hydrogen could act an antiastrogliosis agent during SCI and oxidative in experimental rats cultured astrocytes. Hydrogen-rich saline (HS, 8 mL/kg, i.p.) was injected every 12 h after rats. The expression of STAT3, p-STAT3, glial fibrillary acidic protein (GFAP); the release IL-1β, IL-6, TNF-α; astrogliosis, along with BBB score, were...