A5100448103- Acute Ischemic Stroke Management
- Neurological Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cerebrovascular and Carotid Artery Diseases
- Estrogen and related hormone effects
- Neuroscience and Neuropharmacology Research
- Menopause: Health Impacts and Treatments
- Venous Thromboembolism Diagnosis and Management
- Acute Myocardial Infarction Research
- Alzheimer's disease research and treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Glaucoma and retinal disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Mitochondrial Function and Pathology
- Cardiac, Anesthesia and Surgical Outcomes
- MicroRNA in disease regulation
- Anesthesia and Neurotoxicity Research
- Metabolism, Diabetes, and Cancer
- Retinal Diseases and Treatments
- Hepatitis C virus research
- Autophagy in Disease and Therapy
- Cardiac Valve Diseases and Treatments
- RNA modifications and cancer
- Receptor Mechanisms and Signaling
- Angiogenesis and VEGF in Cancer
West China Hospital of Sichuan University
2014-2025
Peking University First Hospital
2015-2025
Peking University
2012-2025
Sichuan University
2014-2025
Central South University
2025
Xiangya Hospital Central South University
2025
Hunan Institute of Mental Health
2025
University of North Texas Health Science Center
2015-2024
Chinese PLA General Hospital
2018-2024
State Key Laboratory of Kidney Diseases
2021-2024
Estrogen receptors (ERs) are believed to be ligand-activated transcription factors belonging the nuclear receptor superfamily, which on ligand binding translocate into nucleus and activate gene transcription. To date, two ERs have been identified: ERα ERβ. plays major role in estrogen-mediated genomic actions both reproductive nonreproductive tissue, whereas function of ERβ is still unclear. In this study, we used immunocytochemistry, immunoblotting, proteomics demonstrate that localizes...
Neuroprotective strategies, including free radical scavengers, ion channel modulators, and anti-inflammatory agents, have been extensively explored in the last 2 decades for treatment of neurological diseases. Unfortunately, none neuroprotectants has proved effective clinical trails. In current study, we demonstrated that methylene blue (MB) functions as an alternative electron carrier, which accepts electrons from NADH transfers them to cytochrome c bypasses complex I/III blockage. A de...
Testosterone deficiency can lead to depressive symptoms in humans; however, the causes of this are incompletely understood. Here, we isolated Mycobacterium neoaurum from fecal samples testosterone-deficient patients with depression and showed that strain could degrade testosterone vitro. Furthermore, gavaging rats M. reduced their serum brain levels induced depression-like behaviors. We identified gene encoding 3β-hydroxysteroid dehydrogenase (3β-HSD) as enzyme causing degradation....
Ovarian granulosa cells (GCs) proliferate and differentiate along with follicular growth, this is indispensable for oocyte development female fertility. Although the role of macroautophagy/autophagy in ovarian function has been reported, its contribution to regulation GC characteristics remains elusive. The siRNA-mediated knockdown two key autophagy-related genes ATG5 BECN1 autophagy inhibitor chloroquine were used interfere GCs. Inhibition both genetically pharmacologically resulted...
Opioids administered to treat postsurgical pain are a major contributor the opioid crisis, leading chronic use in considerable proportion of patients. Initiatives promoting opioid-free or opioid-sparing modalities perioperative management have led reduced administration operating room, but this reduction could unforeseen detrimental effects terms postoperative outcomes, as relationship between intraoperative usage and later requirements is not well understood.To characterize association...
Previous analyses of fluids collected from chronic, nonhealing wounds found elevated levels inflammatory cytokines, proteinases, and low growth factor activity compared with acute, healing wounds. This led to the general hypothesis that chronic inflammation in acute produces proteinases destroy essential factors, receptors, extracellular matrix proteins, which ultimately prevent healing. To test this further, pro- activated metalloproteinases (MMP-2 MMP-9), tissue inhibitors (TIMP-1 TIMP-2),...
Recent evidence indicates that exosomes can mediate certain microRNAs (miRNAs) involved in a series of biological functions tumor occurrence and development. Our previous studies showed microRNA-21 (miR-21) was abundant both esophageal cancer cells their corresponding exosomes. The present study explored the function exosome-shuttling miR-21 progression. We found could be internalized from extracellular space to cytoplasm. exosome-derived Cy3-labeled mimics transported into recipient neutral...
<p indent="0mm">Atherosclerosis (AS), the main contributor to acute cardiovascular events, such as myocardial infarction and ischemic stroke, is characterized by necrotic core formation plaque instability induced cell death. The mechanisms of death in AS have recently been identified elucidated. Ferroptosis, a novel iron-dependent form death, has proven participate atherosclerotic progression increasing endothelial reactive oxygen species (ROS) levels lipid peroxidation. Furthermore,...
Abstract: PURPOSE: In patients undergoing endovascular thrombectomy (EVT) with acute ischemic stroke (AIS), dynamic cerebral autoregulation (dCA) may minimize neurological injury from blood pressure fluctuations. This study set out to investigate the function of dCA in predicting clinical outcomes following EVT. METHODS: 43 AIS middle or internal carotid artery underwent EVT, and healthy individuals (controls) were enrolled this case control research. The was evaluated using transcranial...
Substantial evidence now exists that intrinsic free-radical scavenging contributes to the receptor-independent neuroprotective effects of estrogens. This activity is inherently associated with presence a phenolic A-ring in steroid. We report previously unrecognized antioxidant cycle maintains “chemical shield” raised by estrogens against most harmful reactive oxygen species, hydroxyl radical (•OH) produced Fenton reaction. In this cycle, capture •OH was shown produce nonphenolic quinol no...
Increasing evidence has demonstrated striking sex differences in the outcome of neurological injury. Whereas estrogens contribute to these by attenuating neurotoxicity and ischemia-reperfusion injury, effects testosterone are unclear. The present study was undertaken determine on neuronal injury both a cell-culture model rodent model. Glutamate-induced HT-22 cell-death used evaluate cell survival. Testosterone shown significantly increase toxicity glutamate at 10 μM concentration, whereas...
Aberrant mitosis occurs in many tauopathy-related neurodegenerative diseases and is believed to precede the formation of neurofibrillary tangles. In this study, we report for first time that transient cerebral ischemia induces aberrant mitotic proteins hyperphosphorylation tau protein with tangle-like conformational epitopes adult female rat cortex. Following rats, initiation apoptosis precedes potentially integrated subsequent hyperphosphorylation. Furthermore, inhibition mitosis-related...
Glioblastoma multiforme (GBM), like most cancers, possesses a unique bioenergetic state of aerobic glycolysis known as the Warburg effect. Here, we documented that methylene blue (MB) reverses effect evidenced by increasing oxygen consumption and reduction lactate production in GBM cell lines. MB decreases proliferation halts cycle S phase. Through activation AMP-activated protein kinase, inactivates downstream acetyl-CoA carboxylase cyclin expression. Structure-activity relationship...
Background and Purpose— Pyruvate is known to be cytoprotective through antioxidant anti-inflammatory mechanisms. We tested the hypothesis that pyruvate protects brain against ischemia–reperfusion injury by inducing endogenous erythropoietin (EPO) expression. Methods— Pyruvate's protective effect was evaluated in C6 glioma cells HT22 neuronal subjected transient oxygen glucose deprivation. Cell viability (calcein AM assay) expression of hypoxia-inducible factor-1α, EPO, Akt Erk (immunoblot),...