A5010932917- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Fungal Biology and Applications
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Carbohydrate Chemistry and Synthesis
- SARS-CoV-2 detection and testing
- Phytochemical compounds biological activities
- Mycobacterium research and diagnosis
- Microbial Fuel Cells and Bioremediation
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Microbial Metabolism and Applications
- RNA and protein synthesis mechanisms
- Algal biology and biofuel production
- Plant Disease Resistance and Genetics
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Structural Characterization
- Advanced biosensing and bioanalysis techniques
Wuhan University
2016-2025
Zhongnan Hospital of Wuhan University
2022-2025
Shanghai Jiao Tong University
2006-2025
State Key Laboratory of Microbial Metabolism
2024
Renmin Hospital of Wuhan University
2023-2024
Wuhan Institute of Bioengineering
2014-2023
Hunan Rice Research Institute
2020-2021
Ministry of Agriculture and Rural Affairs
2020-2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
Northeastern University
2019
Saccharomyces cerevisiae is an efficient host for natural-compound production and preferentially employed in academic studies bioindustries. However, S. exhibits limited capacity lipophilic natural products, especially compounds that accumulate intracellularly, such as polyketides carotenoids, with some engineered displaying cytotoxicity. In this study, we used a nature-inspired strategy to establish effective platform improve lipid oil–triacylglycerol (TAG) metabolism enable increased...
Enzymatic reactions in living cells are highly dynamic but simultaneously tightly regulated. Enzyme engineers seek to construct multienzyme complexes prevent intermediate diffusion, improve product yield, and control the flux of metabolites. Here we choose a pair short peptide tags (RIAD RIDD) create scaffold-free enzyme assemblies achieve these goals. In vitro, assembling enzymes menaquinone biosynthetic pathway through RIAD-RIDD interaction yields protein nanoparticles with varying...
The ongoing global novel coronavirus pneumonia COVID-19 outbreak has engendered numerous cases of infection and death. diagnosis relies upon nucleic acid detection; however, currently recommended methods exhibit high false-negative rates are unable to identify other respiratory virus infections, thereby resulting in patient misdiagnosis impeding epidemic containment. Combining the advantages targeted amplification long-read, real-time nanopore sequencing, herein, sequencing (NTS) is...
Summary Background 2019-Novel coronavirus (2019-nCoV) outbreaks create challenges for hospital laboratories because thousands of samples must be evaluated each day. Sample types, interpretation methods, and corresponding laboratory standards established. The possibility other infections should assessed to provide a basis clinical classification, isolation, treatment. Accordingly, in the present study, we testing methods 2019-nCoV co-infections. Methods We used fluorescence-based quantitative...
All known triterpenes are generated by triterpene synthases (TrTSs) from squalene or oxidosqualene1. This approach is fundamentally different the biosynthesis of short-chain (C10-C25) terpenes that formed polyisoprenyl diphosphates2-4. In this study, two fungal chimeric class I TrTSs, Talaromyces verruculosus talaropentaene synthase (TvTS) and Macrophomina phaseolina macrophomene (MpMS), were characterized. Both enzymes use dimethylallyl diphosphate isopentenyl hexaprenyl as substrates,...
Testosterone deficiency can lead to depressive symptoms in humans; however, the causes of this are incompletely understood. Here, we isolated Mycobacterium neoaurum from fecal samples testosterone-deficient patients with depression and showed that strain could degrade testosterone vitro. Furthermore, gavaging rats M. reduced their serum brain levels induced depression-like behaviors. We identified gene encoding 3β-hydroxysteroid dehydrogenase (3β-HSD) as enzyme causing degradation....
ABSTRACT Approaches using metabolic engineering and synthetic biology to overproduce terpenoids, such as the precursors of taxol artemisinin, in microbial systems have achieved initial success. However, due lack steady‐state kinetic information incomplete understanding terpenoid biosynthetic pathway, it has been difficult build a highly efficient, universal system. Here, we reconstituted mevalonate pathway produce farnesene (a precursor new jet fuel) vitro purified protein components. The...
Microbial fatty acid derivatives are emerging as promising alternatives to fossil fuel derived transportation fuels. Among bacterial synthases (FAS), the Escherichia coli FAS is perhaps most well studied, but little known about its steady-state kinetic behavior. Here we describe reconstitution of E. using purified protein components and report detailed analysis this reconstituted system. When all ketosynthases present at 1 μM, maximum rate free synthesis exceeded 100 μM/ min. The turnover...
Terpenoids represent the largest family of natural products. Their structural diversity is largely due to variable skeletons generated by terpene synthases. However, found in nature are much more than those from known Most promiscuous synthases (i.e. that can generate one product) have not been comprehensively characterised. Here, we first demonstrated produce terpenoids vivo converting precursor polyisoprenoid diphosphates different lengths (C10, C15, C20, C25). To release synthetic...
Lycopene is widely used in foods, cosmetics, nutritional supplements, and pharmaceuticals. Microbial production of lycopene has been intensively studied. However, there are few systematic engineering studies on Saccharomyces cerevisiae aimed at achieving high-yield production. In the current study, by employing a optimization strategy, we screened key biosynthetic genes, crtE, crtB, crtI, from diverse organisms. By adjusting copy number these three knocking out endogenous bypass increasing...
Multienzyme complexes, or metabolons, are assemblies clusters of sequential enzymes that naturally exist in metabolic pathways. These nanomachineries catalyze the conversion metabolites more effectively than freely floating by minimizing diffusion intermediates vivo. Bioengineers have devised synthetic versions multienzyme complexes cells to synergize heterologous biosynthesis, improve intracellular flux, and achieve higher titer valuable chemical products. Here, we utilized orthogonal...
Terpenoids, critical components of human medicine, are the largest family natural products. Fungi an important source terpenoids, but many corresponding biosynthetic gene clusters (BGCs) silent in laboratory conditions. Strategies such as homologous activation and heterologous expression were usually used to active a single cluster, making them low efficiency. Here we developed automated high-throughput (auto-HTP) biofoundry workflow using Aspergillus oryzae chassis that enables efficient...
Vitamin E is one of the most widely used vitamins. In classical commercial synthesis vitamin (α-tocopherol), chemical isophytol key technical barrier. Here, we establish a new process for from microbial fermented farnesene. To achieve an efficient pathway farnesene production, Saccharomyces cerevisiae was selected as host strain. First, β-farnesene synthase genes different sources were screened, and through protein engineering system metabolic engineering, high production in S. achieved...
ABSTRACT The long hydrocarbon fatty acyl chain is energy rich, making it an ideal precursor for liquid transportation fuels and high‐value oleo chemicals. As Saccharomyces cerevisiae has many advantages industrial production compared to Escherichia coli . Here, we attempted engineer overproduction of acids. First, disruption the beta‐oxidation pathway, elimination acyl‐CoA synthetases, overexpression different thioesterases acetyl‐CoA carboxylase ACC1, engineering supply acetyl‐CoA....
With the advent of genomics era, heterologous gene expression has been used extensively as a means accessing natural products (NPs) from environmental DNA samples. However, production NPs often very low efficiency or is unable to produce targeted NPs. Moreover, due complicated transcriptional and metabolic regulation NP biosynthesis in native producers, especially cases genome mining, it also difficult rationally systematically engineer synthetic pathways improved biosynthetic efficiency. In...
Based on a terpenoid overproduction platform in yeast for genome mining, chimeric diterpene synthase from the endophytic fungus Colletotrichum gloeosporioides ES026 was characterized as (5R,12R,14S)-dolasta-1(15),8-diene synthase. The absolute configuration independently verified through use of enantioselectively deuterated terpene precursors, which unequivocally established predicted C1-III-IV cyclization mode this first clade II-D enzyme. Extensive isotopic labeling experiments and...