A5061707731- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Plant Pathogens and Fungal Diseases
- Natural product bioactivities and synthesis
- Microbial Metabolic Engineering and Bioproduction
- Pharmacological Effects of Natural Compounds
- Plant Disease Resistance and Genetics
- Phytochemical compounds biological activities
- Fungal Biology and Applications
- Microbial Fuel Cells and Bioremediation
- Essential Oils and Antimicrobial Activity
- Photosynthetic Processes and Mechanisms
- Actinomycetales infections and treatment
- Sesquiterpenes and Asteraceae Studies
- Plant Gene Expression Analysis
- Cancer therapeutics and mechanisms
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- bioluminescence and chemiluminescence research
- Photoreceptor and optogenetics research
- Ginger and Zingiberaceae research
- Infectious Diseases and Mycology
- Biofuel production and bioconversion
Shenzhen Institutes of Advanced Technology
2023-2024
Chinese Academy of Sciences
2023-2024
Wuhan University
2017-2023
State Key Laboratory of Synthetic Chemistry
2023
Zhongnan Hospital of Wuhan University
2022
Jiangsu Normal University
2010-2013
All known triterpenes are generated by triterpene synthases (TrTSs) from squalene or oxidosqualene1. This approach is fundamentally different the biosynthesis of short-chain (C10-C25) terpenes that formed polyisoprenyl diphosphates2-4. In this study, two fungal chimeric class I TrTSs, Talaromyces verruculosus talaropentaene synthase (TvTS) and Macrophomina phaseolina macrophomene (MpMS), were characterized. Both enzymes use dimethylallyl diphosphate isopentenyl hexaprenyl as substrates,...
Terpenoids represent the largest family of natural products. Their structural diversity is largely due to variable skeletons generated by terpene synthases. However, found in nature are much more than those from known Most promiscuous synthases (i.e. that can generate one product) have not been comprehensively characterised. Here, we first demonstrated produce terpenoids vivo converting precursor polyisoprenoid diphosphates different lengths (C10, C15, C20, C25). To release synthetic...
Terpenoids, critical components of human medicine, are the largest family natural products. Fungi an important source terpenoids, but many corresponding biosynthetic gene clusters (BGCs) silent in laboratory conditions. Strategies such as homologous activation and heterologous expression were usually used to active a single cluster, making them low efficiency. Here we developed automated high-throughput (auto-HTP) biofoundry workflow using Aspergillus oryzae chassis that enables efficient...
Based on a terpenoid overproduction platform in yeast for genome mining, chimeric diterpene synthase from the endophytic fungus Colletotrichum gloeosporioides ES026 was characterized as (5R,12R,14S)-dolasta-1(15),8-diene synthase. The absolute configuration independently verified through use of enantioselectively deuterated terpene precursors, which unequivocally established predicted C1-III-IV cyclization mode this first clade II-D enzyme. Extensive isotopic labeling experiments and...
Significance Chimeric terpene synthases, termed PTTSs, are a unique family of enzymes occurring only in fungi. Characterizing PTTSs is challenging due to the complex reactions they catalyze and structural complexity their products. Here, by devising an efficient precursor-providing yeast chassis incorporating high-throughput automated platform, we identified 34 active which was considerably more than number known functional PTTSs. This effective rapid pipeline can be employed for...
Microbial communication can drive coordinated functions through sensing, analyzing and processing signal information, playing critical roles in biomanufacturing life evolution. However, it is still a great challenge to develop effective methods construct microbial system with behaviors. Here, we report an electron transfer triggered redox network consisting of three building blocks including router, optical verifier bio-actuator for metabolism regulation coordination. In the network,...
Herein, we report a short semisynthesis of the potent transient receptor potential canonical (TRPC) channel agonist englerin A (EA) and related guaianes oxyphyllol orientalol E. The guaia-6,10(14)-diene starting material was systematically engineered in Escherichia coli Saccharomyces cerevisiae using CRISPR/Cas9 system produced with high titers. potentially scalable approach combines advantages synthetic biology chemical synthesis providing an efficient economical method for producing EA analogues.
Abstract Catalytic versatility is an inherent property of many enzymes. In nature, terpene cyclases comprise the foundation molecular biodiversity as they generate diverse hydrocarbon scaffolds found in thousands terpenoid natural products. Here, we report that catalytic activity AaTPS and FgGS can be switched from cyclase to aromatic prenyltransferase at basic pH prenylindoles. The crystal structures provide insights into mechanism this cryptic function. Moreover, discovered other indicates...
Abstract Microbial cell factories reinvigorate current industries by producing complex fine chemicals at low costs. Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is the main reducing power to drive biosynthetic pathways in microorganisms. However, insufficient intrinsic NADPH limits productivity of Here, we report that supplying microorganisms with long‐lived electrons from persistent phosphor mesoporous Al 2 O 3 (meso‐Al ) can elevate level facilitate efficient chemical...
Taxol (paclitaxel) is a diterpenoid compound with significant and extensive applications in the treatment of cancer. The production relevant intermediates by engineered microbes an attractive alternative to semichemical synthesis Taxol. In this study, based on previously developed platform, authors first established taxadiene mutant E. coli T2 T4 engineering mevalonate (MVA) pathway. then Agrobacterium tumefaciens-mediated transformation (ATMT) method verified strength heterologous promoters...
A novel actinomycete, designated strain KLBMP 1279 T , was isolated from surface-sterilized roots of a coastal halophyte, Salicornia europaea Linn., collected Jiangsu Province, in the east China. The taxonomic status this organism established using polyphasic approach. 16S rRNA gene sequence analysis indicated that closely related to Modestobacter marinus 42H12-1 (99.5 % similarity), versicolor CP153-2 (98.4 %) and multiseptatus AA-826 (97.5 %). Chemotaxonomic characteristics were consistent...
The potential power of sesquiterpene synthase FgJ03939 from Fusarium graminearum was fully exploited in a farnesyl diphosphate-overexpressing Saccharomyces cerevisiae chassis to produce the novel sesquiterpenes fusariumdiene (1), epi-fusagramineol (2), and fusagramineol (3) with 5/7 bicyclic 5/6/3 tricyclic ring systems, respectively, as well five known (4–8). structure unusual skeletons characterized, an absolute configuration proposed. A mechanism for biosynthesis 1–8 also
(+)-Nootkatone is a natural sesquiterpene ketone widely used in food, cosmetics, pharmaceuticals, and agriculture. It also regarded as one of the most valuable terpenes commercially. However, plants contain trace amounts (+)-nootkatone, extraction from insufficient to meet market demand. Alpinia oxyphylla well-known medicinal plant China, (+)-nootkatone main components within fruits. By transcriptome mining functional screening using precursor-providing yeast chassis, complete biosynthetic...
Tremulane sesquiterpenoids are key secondary metabolites of the basidiomycete Irpex lacteus, which displays structural diversity and various bioactivities. However, tremulane sesquiterpene synthases have not been reported to date. The synthase I. lacteus was characterized by genome mining, heterologous expression, an in vitro assay, substrate feeding. Moreover, structures corresponding products were elucidated NMR spectroscopy X-ray diffraction analysis.
A novel actinomycete, designated strain KLBMP 1256 T , was isolated from a surface-sterilized stem of the oil-seed plant Jatropha curcas L. collected Sichuan Province, south-west China, and characterized to determine its taxonomic position. Phylogenetic analyses based on 16S rRNA gene sequences indicated that isolate closely related members genus Nocardia in family Nocardiaceae being most callitridis CAP 290 (98.4 % similarity) nova JCM 6044 (97.5 %). Levels sequence similarity between type...