A5100400758- Ubiquitin and proteasome pathways
- Spatial Neglect and Hemispheric Dysfunction
- Protein Degradation and Inhibitors
- Advancements in Battery Materials
- NF-κB Signaling Pathways
- Agronomic Practices and Intercropping Systems
- Cognitive and developmental aspects of mathematical skills
- Plant Gene Expression Analysis
- Microbial Natural Products and Biosynthesis
- Plant nutrient uptake and metabolism
- Lipid metabolism and biosynthesis
- Immune cells in cancer
- Viral gastroenteritis research and epidemiology
- Endoplasmic Reticulum Stress and Disease
- Histone Deacetylase Inhibitors Research
- Plant-Microbe Interactions and Immunity
- Nitrogen and Sulfur Effects on Brassica
- Transgenic Plants and Applications
- Antibiotics Pharmacokinetics and Efficacy
- Mathematics Education and Teaching Techniques
- Phytase and its Applications
- Estrogen and related hormone effects
- Pharmacogenetics and Drug Metabolism
- Osteoarthritis Treatment and Mechanisms
- Immunotherapy and Immune Responses
Zhengzhou People's Hospital
2025
Changzhi University
2024
The University of Tokyo
2024
Zhengzhou University
2023
Center for Drug Evaluation and Research
2023
Wuhan University
2022
Shanxi University
2022
Shandong Agricultural University
2022
Shanghai Jiao Tong University
2021
South China Normal University
2013-2021
All known triterpenes are generated by triterpene synthases (TrTSs) from squalene or oxidosqualene1. This approach is fundamentally different the biosynthesis of short-chain (C10-C25) terpenes that formed polyisoprenyl diphosphates2-4. In this study, two fungal chimeric class I TrTSs, Talaromyces verruculosus talaropentaene synthase (TvTS) and Macrophomina phaseolina macrophomene (MpMS), were characterized. Both enzymes use dimethylallyl diphosphate isopentenyl hexaprenyl as substrates,...
Abstract Ubiquitin-specific protease 14 (USP14) is one of the major proteasome-associated deubiquitinating enzymes critical for proteome homeostasis. However, substrates USP14 remain largely unknown, hindering understanding its functional roles. Here we conduct a comprehensive proteome, ubiquitinome and interactome analysis substrate screening. Bioinformatics reveals broad new potential roles USP14, especially in lipid carbohydrate metabolism. Among identified, show that fatty acid synthase...
Kainate receptors have been implicated in excitotoxic neuronal death induced by diseases such as epilepsy and stroke. Actinfilin, a synaptic member of the BTB-Kelch protein family, is known to bind actin cytoskeleton. However, little understood about its function at synapse. Here, we report that actinfilin able GluR6, kainate-type glutamate receptor subunit, target GluR6 for degradation. Like many members acts substrate adaptor, binding Cullin 3 (Cul3) linking E3 ubiquitin-ligase complex. We...
The linear relationship of Nov GII VP1 protein in the range 2.5–320 ng mL −1 was Y = 0.6784 X − 1.1443, R 2 0.9935 and lowest limit detection 0.61 .
Ovarian cancer is the most lethal gynecologic malignancy worldwide. Olaparib, an inhibitor of poly (ADP-ribose) polymerase (PARP), becoming widely used in ovarian treatment. The overall survival has not been significantly changed over past decades and become increasingly resistant to Olaparib. Ubiquitin-conjugating enzyme E2S (UBE2S) proved promote malignant behaviors many cancers. However, function UBE2S development Olaparib resistance are unclear.In this study, we detected expression...
Multiple myeloma (MM) is an incurable hematologic malignancy due to inevitable relapse and chemoresistance development. Our preliminary data show that MM cells express high levels of PGC1β LDHA. In this study, we investigated the mechanism behind PGC1β-mediated LDHA expression its contribution tumorigenesis, aid in development novel therapeutic approaches for MM. Real-time PCR western blotting were first used evaluate gene different cells, then, luciferase reporter assay, chromatin...
Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated 9 (Cas9)-mediated loss-of-function screens are powerful tools for identifying genes responsible diverse phenotypes. Here, we perturbed in melanoma cells to screen involved tumor escape from T cell-mediated killing. Multiple interferon gamma (IFNγ) signaling-related were enriched resistant cell In addition, deletion of the deubiquitinating protease ubiquitin specific peptidase 22 (USP22) mouse...
Abstract Protein S-acylation is an important post-translational modification in eukaryotes, regulating the subcellular localization, trafficking, stability, and activity of substrate proteins. The dynamic regulation this reversible mediated inversely by protein S-acyltransferases de-S-acylation enzymes, but mechanism remains unclear plant cells. Here, we characterized a group putative enzymes Arabidopsis thaliana, including 11 members Alpha/Beta Hydrolase Domain-containing 17-like acyl...
Many studies have shown that solving addition and subtraction problems can induce overt shifts of spatial attention. In particular, right-side targets are detected faster than left-side when preceded by an operation, while a operation. However, the interaction between space arithmetic in multiplication or division is hardly studied remains controversial. order to make strong case for mental arithmetic, we attempted replicate spatial-arithmetic association (Experiment 1), at same time...
Ubiquitin-fold modifier-1 (Ufm1) is a recently identified ubiquitin-like protein. We previously confirmed that Ufm1 expression was increased in diabetic mice. However, its role the development of diabetes remains undefined.Lentivirus-mediated gene knockdown and overexpression techniques were used to observe effect on inflammatory factors, adhesion molecules chemokines, as well transcriptional activity nuclear factor kappa-B (NF-κB) macrophages. Western blot immunofluorescence analyses...
Chlorophyll contributes to tea coloration, which is an important factor in quality. metabolism induced by light, but the transcriptional regulation responsible for light-induced chlorophyll largely unknown leaves. Here, we characterized a chlorophyllase1 gene CsCLH1 from young leaves and showed it essential metabolism, using transient overexpression silencing ectopic Arabidopsis. was significantly high light. The DOF protein CsDOF3, upstream direct regulator of CsCLH1, also identified....
Sulfur-containing compounds are essential for plant development and environmental adaptation, closely related to the flavor nutrition of agricultural products. Cysteine, first organic sulfur-containing molecule generated in plants, is precursor most these active substances. Serine acetyltransferase (SERAT) catalyzes rate-limiting step its formation. However, despite their importance, systematic analyses enzymes individual species, especially economically important crops, still limited. Here,...
Geminiviruses are DNA viruses that cause severe diseases in diverse species of plants, resulting considerable agricultural losses worldwide. C4 proteins a major symptom determinant several geminiviruses, including Beet curly top virus (BSCTV). Here, we uncovered novel mechanism by which danger peptide signaling enhances the internalization BSCTV plant cells. Previous studies showed this is important for activation bacterium- and fungus-triggered immune responses, but its function plant-virus...
F-box protein S-phase kinase-associated 2 (Skp2) is a component of cullin-RING ligases, which responsible for recruiting and ubiquitinating substrates subsequently plays its proteolytic non-proteolytic role. High expression Skp2 frequently observed in multiple aggressive tumor tissues associated with poor prognosis. Several the inhibitors have been reported last decades; however, few them shown detailed structure–activity relationship (SAR) potent bioactivity. Herein, based on hit compound...
This study aimed to gain a better understanding of the molecular circuitry Schmid-type metaphyseal chondrodysplasia (SMCD), and identify more potential genes associated with pathogenesis SMCD. Microarray data from GSE72261 were downloaded NCBI GEO database, including collagen X p.Asn617Lys knock-in mutation (ColXN617K), ablated XBP1 activity (Xbp1CartΔEx2), compound mutant (C/X), wild-type (WT) specimens. Differentially expressed (DEGs) screened in Xbp1 vs. WT, Col WT CX respectively....
Cephalosporins belong the largest class of antibiotics used in treatment a wide range infectious diseases caused by susceptible organisms. In present study, we chose two typical cefalexin/cefixime based on their structure, and investigated interaction cephalexin/cefixime with bovine serum albumin (BSA) using UV-vis absorption spectra, fluorescence spectroscopy, circular dichroism (CD) spectroscopy molecular modeling approaches. Spectroscopic experiments revealed formation BSA - complex. The...
F-Box and WD40 domain containing protein 7 gene (FBXW7) is part of the E3 ubiquitin ligase complex that controls turnover various proteins including NOTCH1, c-MYC Cyclin E.To investigate mutations FBXW7 in adult T-cell acute lymphoblastic leukemia (T-ALL).Exon 5-12 were amplified, cloned sequenced 54 T-ALL patients; frequency, position types mutation analyzed; co-existing with NOTCH1 their relevant prognostic significance explored as well.FBXW7 identified 11.1% patients. A total 4 point...
Owing to their structural diversity and mesoporous construction, metal-organic frameworks (MOFs) have been used as templates prepare metal oxides, which show excellent performance anode materials for lithium-ion batteries (LIBs). Co-ZnO/C Co-Co