A5102012954- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Ubiquitin and proteasome pathways
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Plant Parasitism and Resistance
- Plant responses to water stress
- Genetic Mapping and Diversity in Plants and Animals
- Plant Gene Expression Analysis
- Bioenergy crop production and management
- Cellular transport and secretion
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Calcium signaling and nucleotide metabolism
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Protein Degradation and Inhibitors
- Insect-Plant Interactions and Control
Institute of Genetics and Developmental Biology
2016-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2016-2025
Guangdong Academy of Agricultural Sciences
2023-2025
Syngenta (China)
2023-2025
Shenzhen University
2025
State Key Laboratory of Plant Genomics
2015-2024
Huazhong Agricultural University
2012-2024
Sun Yat-sen University
2004-2024
Institute of Vegetables and Flowers
2022
Although several plant microRNAs (miRNAs) have been shown to play a role in development, no phenotype has yet associated with reduction or loss of expression any miRNA. Arabidopsis thaliana miR164 was predicted target five NAM/ATAF/CUC (NAC) domain-encoding mRNAs, including NAC1, which transduces auxin signals for lateral root emergence. Here, we show that guides the cleavage endogenous and transgenic NAC1 mRNA, producing 3'-specific fragments. Cleavage blocked by mutations disrupt base...
Auxin plays a key role in lateral root formation, but the signaling pathway for this process is poorly understood. We show here that NAC1 , new member of NAC family, induced by auxin and mediates to promote development. transcription activator consisting an N-terminal conserved NAC-domain binds DNA C-terminal activation domain. This factor activates expression two downstream auxin-responsive genes, DBP AIR3 . Transgenic plants expressing sense or antisense cDNA increase reduction roots,...
Plant responses to cold stress are mediated by a transcriptional cascade, in which the transcription factor ICE1 and possibly related proteins activate expression of C-repeat (CRT)-binding factors (CBFs), leading downstream effector genes. The variant RING finger protein high osmotically responsive gene (HOS)1 was identified genetically as negative regulator responses. We present evidence here that HOS1 is an E3 ligase required for ubiquitination ICE1. physically interacts with mediates both...
During plant development, the final size of an organ is regulated and determined by various developmental signals; however, molecular mechanisms which these signals are transduced mediators involved largely unknown. Here, we show that ARGOS, a novel Arabidopsis gene highly induced auxin, in control. Transgenic plants expressing sense or antisense ARGOS cDNA display enlarged reduced aerial organs, respectively. The alteration attributable mainly to changes cell number duration growth. Ectopic...
Abstract The rice (Oryza sativa) spotted leaf11 (spl11) mutant was identified from an ethyl methanesulfonate–mutagenized indica cultivar IR68 population and previously shown to display a spontaneous cell death phenotype enhanced resistance fungal bacterial pathogens. Here, we have isolated Spl11 via map-based cloning strategy. isolation of the gene facilitated by identification three additional spl11 alleles IR64 collection. predicted SPL11 protein contains both U-box domain armadillo (ARM)...
Summary Rice tillering is an important agronomic trait for grain production. The HIGH‐TILLERING DWARF1 ( HTD1 ) gene encodes ortholog of Arabidopsis MAX3. Complementation analyses confirm that the defect in responsible both high‐tillering and dwarf phenotypes htd1 mutant. rescue max3 mutant phenotype by introduction Pro 35S :HTD1 indicates a carotenoid cleavage dioxygenase has same function as MAX3 synthesis carotenoid‐derived signal molecule. expressed shoot root tissues. By evaluating HTD...
Ubiquitination plays important roles in plant hormone signal transduction. We show that the RING finger E3 ligase, Arabidopsis thaliana SALT- AND DROUGHT-INDUCED FINGER1 (SDIR1), is involved abscisic acid (ABA)-related stress SDIR1 expressed all tissues of and upregulated by drought salt stress, but not ABA. Plants expressing ProSDIR1-beta-glucuronidase (GUS) reporter construct confirmed strong induction GUS expression stomatal guard cells leaf mesophyll under stress. The green fluorescent...
Seed dormancy is an important economic trait for agricultural production. Abscisic acid (ABA) and Gibberellins (GA) are the primary factors that regulate transition from to germination, they this process antagonistically. The detailed regulatory mechanism involving crosstalk between ABA GA, which underlies seed dormancy, requires further elucidation. Here, we report ABI4 positively regulates while negatively regulating cotyledon greening, by mediating biogenesis of GA. Seeds Arabidopsis abi4...
Abstract The genus Brassica includes several important agricultural and horticultural crops. Their current genome structures were shaped by whole-genome triplication followed extensive diploidization. availability of crucifer sequences, especially that Chinese cabbage (Brassica rapa), enables study the evolution mesohexaploid genomes from their diploid progenitors. We reconstructed three ancestral subgenomes B. rapa (n = 10) comparing its sequence to extant Brassicaceae genomes. All...
RNA interference (RNAi) induced through double stranded (dsRNA) has been used widely to study gene function in insects. Recently, it reported that knockdown several insects can be successfully feeding with dsRNA. However, is still unknown whether phenotypic silencing of genes not expressed the midgut occurs after ingestion insect dsRNA.Using chitin synthase A (SeCHSA) as target gene, which cuticle and tracheae lepidopteran pest Spodoptera exigua, we showed growth development S. exigua larvae...
The ubiquitination proteasome pathway has been demonstrated to regulate all plant developmental and signaling processes. E3 ligase/substrate-specific interactions play important roles in this pathway. However, due technical limitations only a few instances of ligase-substrate binding protein plants have directly evidenced. An efficient vivo vitro assay was developed for analysis reactions by agroinfiltration expression both substrates ligases Nicotiana benthamiana. Using detailed the...
Plants have evolved intricate immune mechanisms to combat pathogen infection. Upon perception of pathogen-derived signals, plants accumulate defense hormones such as ethylene (ET), jasmonate, salicylate, and damage-associated molecular patterns amplify responses. In particular, the Arabidopsis peptide Pep1 its family members are thought be that trigger immunity through receptor kinases PEPR1 PEPR2. Here we show specifically interacts with receptor-like cytoplasmic botrytis-induced kinase 1...
Thellungiella salsuginea, a close relative of Arabidopsis , represents an extremophile model for abiotic stress tolerance studies. We present the draft sequence T. salsuginea genome, assembled based on ∼134-fold coverage to seven chromosomes with coding capacity at least 28,457 genes. This genome provides resources and evidence about nature defense mechanisms constituting genetic basis underlying plant tolerance. Comparative genomics experimental analyses identified genes related cation...
Abstract Clade A protein phosphatase 2Cs (PP2Cs) are abscisic acid (ABA) co-receptors that block ABA signalling by inhibiting the downstream kinases. is activated after PP2Cs inhibited ABA-bound PYR/PYL/RCAR receptors (PYLs) in Arabidopsis . However, whether these regulated other factors remains unknown. Here, we report ABI1 (ABA-INSENSITIVE 1) can interact with U-box E3 ligases PUB12 and PUB13, but ubiquitinated only when it interacts an vitro assay. mutant form of ABI1-1 unable to PYLs...
Abstract De novo organ regeneration is an excellent biological system for the study of fundamental questions regarding stem cell initiation, fate determination, and hormone signaling. Despite general belief that auxin cytokinin responses interact to regulate de regeneration, molecular mechanisms underlying such a cross talk are little understood. Here, we show spatiotemporal biosynthesis polar transport resulted in local distribution Arabidopsis (Arabidopsis thaliana), which turn determined...
Light and brassinosteroid (BR) antagonistically regulate the developmental switch from etiolation in dark to photomorphogenesis light plants. Here, we identify GATA2 as a key transcriptional regulator that mediates crosstalk between BR- light-signaling pathways. Overexpression of causes constitutive dark, whereas suppression reduces caused by light, BR deficiency, or mutant cop1. Genome profiling chromatin immunoprecipitation experiments show directly regulates genes respond both BR....