A5090953273- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Protein Degradation and Inhibitors
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Advanced Welding Techniques Analysis
- Autophagy in Disease and Therapy
- Plant Virus Research Studies
- Aluminum Alloys Composites Properties
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- 14-3-3 protein interactions
- Glycosylation and Glycoproteins Research
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Agricultural Engineering and Mechanization
- Aluminum Alloy Microstructure Properties
- Plant Disease Resistance and Genetics
- Plant Surface Properties and Treatments
- Electronic Packaging and Soldering Technologies
- Allelopathy and phytotoxic interactions
- Click Chemistry and Applications
Liaocheng University
2008-2024
Shandong University
2005-2024
Institute of Genetics and Developmental Biology
2007-2022
Chinese Academy of Sciences
2007-2022
State Key Laboratory of Plant Genomics
2007-2022
Washington University in St. Louis
2019
Hunan Agricultural University
2011-2012
The Ohio State University
2011-2012
Chinese Academy of Agricultural Sciences
2011-2012
Institute of Plant Protection
2011-2012
Ubiquitination plays important roles in plant hormone signal transduction. We show that the RING finger E3 ligase, Arabidopsis thaliana SALT- AND DROUGHT-INDUCED FINGER1 (SDIR1), is involved abscisic acid (ABA)-related stress SDIR1 expressed all tissues of and upregulated by drought salt stress, but not ABA. Plants expressing ProSDIR1-beta-glucuronidase (GUS) reporter construct confirmed strong induction GUS expression stomatal guard cells leaf mesophyll under stress. The green fluorescent...
The ubiquitination proteasome pathway has been demonstrated to regulate all plant developmental and signaling processes. E3 ligase/substrate-specific interactions play important roles in this pathway. However, due technical limitations only a few instances of ligase-substrate binding protein plants have directly evidenced. An efficient vivo vitro assay was developed for analysis reactions by agroinfiltration expression both substrates ligases Nicotiana benthamiana. Using detailed the...
Abstract Plants modify their growth and development to protect themselves from detrimental conditions by triggering a variety of signaling pathways, including the activation ubiquitin-mediated protein degradation pathway. Endoplasmic reticulum (ER)-associated (ERAD) is an important aspect ubiquitin-proteasome system, but only few active ERAD components have been reported in plants. Here, we report that Arabidopsis thaliana ubiquitin-conjugating enzyme, UBC32, stress-induced functional...
Plant viruses are excellent tools for studying microbial-plant interactions as well the complexities of host activities. Our study focuses on role C2 encoded by Beet severe curly top virus (BSCTV) in virus-plant interaction. Using BSCTV bait a yeast two-hybrid screen, C2-interacting protein, S-adenosyl-methionine decarboxylase 1 (SAMDC1), was identified from an Arabidopsis thaliana cDNA library. The interaction confirmed vitro pull-down assay and firefly luciferase complemention imaging...
Ubiquitin-regulated protein degradation is a critical regulatory mechanism that controls wide range of biological processes in plants. Here, we report OsDIS1 (for Oryza sativa drought-induced SINA 1), C3HC4 RING finger E3 ligase, involved drought-stress signal transduction rice (O. sativa). The expression was up-regulated by drought treatment. In vitro ubiquitination assays showed possessed ubiquitin ligase activity and the conserved region required for activity. Transient Nicotiana...
Abstract The phytohormone abscisic acid (ABA) is well known for its regulatory roles in integrating environmental constraints with the developmental programs of plants. Here, we characterize biological function Arabidopsis (Arabidopsis thaliana) RING-H2 protein RHA2a ABA signaling. rha2a mutant less sensitive to than wild type during seed germination and early seedling development, whereas transgenic plants overexpressing are hypersensitive, indicating that positively regulates ABA-mediated...
Abstract We have previously shown that the Arabidopsis (Arabidopsis thaliana) RING-H2 E3 ligase RHA2a positively regulates abscisic acid (ABA) signaling during seed germination and postgerminative growth. Here, we report RHA2b, closest homolog of RHA2a, is also an active plays important role in ABA signaling. show RHA2b expression induced by overexpression leads to ABA-associated phenotypes such as hypersensitivity seedling growth, enhanced stomatal closure, reduced water loss, and,...
Abstract The components in plant signal transduction pathways are intertwined and affect each other to coordinate growth, development, defenses stresses. role of ubiquitination connecting these pathways, particularly innate immunity flowering, is largely unknown. Here, we report the dual roles for Arabidopsis (Arabidopsis thaliana) Plant U-box protein13 (PUB13) defense flowering time control. In vitro assays indicated that PUB13 an active E3 ubiquitin ligase intact domain required activity....
Cuticular wax plays crucial roles in protecting plants from environmental stresses, particularly drought stress. Many enzyme-encoding genes and transcription factors involved biosynthesis have been identified, but the underlying posttranslational regulatory mechanisms are poorly understood. Here, we demonstrate that DROUGHT HYPERSENSITIVE (DHS), encoding a Really Interesting New Gene (RING)-type protein, is critical regulator of rice (Oryza sativa). The cuticular contents were significantly...
Summary The C4 protein from Curtovirus is known as a major symptom determinant, but the mode of action remains unclear. To understand mechanism involvement in virus–plant interactions, we introduced gene Beet severe curly top virus (BSCTV) into Arabidopsis under conditional expression promoter; resulting overexpression BSCTV led to abnormal host cell division. RKP, RING finger protein, which homolog human cycle regulator KPC1, was discovered be induced by protein. Mutation RKP reduced...
Protein ubiquitination requires the concerted action of three enzymes: ubiquitin-activating enzyme (E1), ubiquitin-conjugating (E2) and ubiquitin ligase (E3). These enzymes belong to an abundant protein family that is encoded in all eukaryotic genomes. Describing their biochemical characteristics important part functional analysis. It has been recognized various E2/E3 specificities exist, detection E3 activity vitro may depend on recruitment E2s. Here, we describe development system based...
Salt stress is a detrimental factor for plant growth and development. The response to salt has been shown involve components in the intracellular trafficking system, as well of ubiquitin-proteasome system (UPS). In this article, we have identified Arabidopsis thaliana little reported ubiquitin ligase involved salt-stress response, which named STRF1 (Salt Tolerance RING Finger 1). member RING-H2 finger proteins demonstrate that it activity vitro. We also show localizes mainly at plasma...
Ubiquitination-mediated protein degradation in both the 26 S proteasome and vacuole is an important process abscisic acid (ABA) signaling. However, role of deubiquitination this remains elusive. Here, we demonstrate that two deubiquitinating enzymes (DUBs), ubiquitin-specific protease 12 (UBP12) UBP13, modulate ABA signaling drought tolerance by stabilizing endosomal sorting complex required for transport-I (ESCRT-I) component vacuolar 23A (VPS23A) thereby affect stability receptors...
Ubiquitination is essential for many cellular processes in eukaryotes, including 26S proteasome-dependent protein degradation, cell cycle progression, transcriptional regulation, and signal transduction. Although numerous ubiquitinated proteins have been empirically identified, their cognate ubiquitin E3 ligases remain largely unknown.
Abstract Abscisic acid (ABA) signaling is crucial for plant responses to various abiotic stresses. The Arabidopsis (Arabidopsis thaliana) transcription factor ABA INSENSITIVE 5 (ABI5) a central regulator of signaling. ABI5 BINDING PROTEIN 1 (AFP1) interacts with and facilitates its 26S-proteasome-mediated degradation, although the detailed mechanism has remained unclear. Here, we report that an ABA-responsive U-box E3 ubiquitin ligase, PLANT U-BOX 35 (PUB35), physically AFP1 ABI5. PUB35...
Abstract Peroxisomes are essential eukaryotic organelles that mediate various metabolic processes. Peroxisome import depends on a group of peroxisome biogenesis factors called peroxins, many which evolutionarily conserved. PEX2, PEX10, and PEX12 three RING‐finger‐domain‐containing integral membrane peroxins crucial for protein import. In yeast ( Saccharomyces cerevisae ), RING act as E3 ligases, facilitating the recycling receptor PEX5 through ubiquitination. plants, to plant vitality. To...
Ubiquitination is an important post-translational protein modification that known to play critical roles in diverse biological processes eukaryotes. The RING E3 ligases function ubiquitination pathways, and are involved a large diversity of physiological higher plants. domain-containing ligase AtRDUF1 was previously identified as positive regulator ABA-mediated dehydration stress response Arabidopsis. In this study, we report plant responses salt stress. expression upregulated by treatment....