A5115602062- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Genetics and Plant Breeding
- Plant tissue culture and regeneration
- MicroRNA in disease regulation
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Crop Yield and Soil Fertility
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Plant Micronutrient Interactions and Effects
- Phytase and its Applications
- Cell Adhesion Molecules Research
- Celiac Disease Research and Management
- Genetics and Neurodevelopmental Disorders
- Yeasts and Rust Fungi Studies
Henan Agricultural University
2016-2025
Tongji Hospital
2009-2025
Huazhong University of Science and Technology
2009-2025
Zhejiang University
2004-2025
Zhejiang Medicine (China)
2025
Peking University
2013-2025
Sir Run Run Shaw Hospital
2025
Sichuan University
2024
State Key Laboratory of Oral Diseases
2024
Beijing Jiaotong University
2024
The genome sequence and its analysis of the diploid wild wheat Triticum urartu (progenitor A genome) represent a tool for studying complex, polyploid genomes should be valuable resource genetic improvement wheat. hexaploid bread aestivum, designated AABBDD, evolved as result hybridization between three ancestral grasses. Two papers published in issue Nature present sequences two these progenitors. First, T. (ancestor genome), which resembles cultivated more strongly than either Aegilops...
Cytosine and adenine base editors (CBEs ABEs) are promising new tools for achieving the precise genetic changes required disease treatment trait improvement. However, genome-wide unbiased analyses of their off-target effects in vivo still lacking. Our whole-genome sequencing analysis rice plants treated with third-generation editor (BE3), high-fidelity BE3 (HF1-BE3), or ABE revealed that HF1-BE3, but not ABE, induce substantial mutations, which mostly C→T type single-nucleotide variants...
Triticum urartu (diploid, AA) is the progenitor of A subgenome tetraploid (Triticum turgidum, AABB) and hexaploid aestivum, AABBDD) wheat1,2. Genomic studies T. have been useful for investigating structure, function evolution polyploid wheat genomes. Here we report generation a high-quality genome sequence by combining bacterial artificial chromosome (BAC)-by-BAC sequencing, single molecule real-time whole-genome shotgun sequencing3, linked reads optical mapping4,5. We assembled seven...
Summary GW 2 is emerging as a key genetic determinant of grain weight in cereal crops; it has three homoeologs ( Ta 2‐A1 , ‐B1 and ‐D1 ) hexaploid common wheat Triticum aestivum L.). Here, by analyzing the gene editing mutants that lack one B1 or D1 ), two all A1 several insights are gained into functions 2‐B1 traits. First, both affect thousand‐grain TGW influencing width length, but effect conferred stronger than 2‐D1 . Second, there exists functional interaction between because increase...
Abstract Rye is a valuable food and forage crop, an important genetic resource for wheat triticale improvement indispensable material efficient comparative genomic studies in grasses. Here, we sequenced the genome of Weining rye, elite Chinese rye variety. The assembled contigs (7.74 Gb) accounted 98.47% estimated size (7.86 Gb), with 93.67% (7.25 assigned to seven chromosomes. Repetitive elements constituted 90.31% genome. Compared previously Triticeae genomes, Daniela , Sumaya Sumana...
In higher eukaryotes, heterochromatin is mainly composed of transposable elements (TEs) silenced by epigenetic mechanisms. But, the silencing certain heterochromatin-associated TEs disrupted heat stress. By comparing genome-wide high-resolution chromatin packing patterns under normal or conditions obtained through Hi-C analysis, we show here that stress causes global rearrangement 3D genome in Arabidopsis thaliana. Contacts between pericentromeric regions and distal chromosome arms, as well...
Our previous results demonstrated that the DNAbeta satellite (Y10beta) associated with Tomato yellow leaf curl China virus Y10 isolate (TYLCCNV-Y10) is essential for induction of symptoms in plants and transgenic expression its betaC1 gene Nicotiana induces virus-like symptoms. In present study, vitro DNA binding activity proteins Y10beta (Y35beta) found Tobacco curly shoot Y35 (TbCSV-Y35) were studied following their as six-His fusion Escherichia coli. Electrophoretic mobility shift assays...
Purple acid phosphatases (PAPs) are members of the metallo-phosphoesterase family. They characterized by presence seven conserved amino residues involved in coordinating dimetal nuclear center their reactive site. We compared 29 PAPs predicted for <i>Arabidopsis thaliana</i> varieties potential metal-ligating residues. Although 24 possessed sets typical known PAPs, 1 member lacked four For remaining members, were generally more similar to those metal-dependent exonucleases and related...
Abstract Induction of secreted acid phosphatase (APase) is a universal response higher plants to phosphate (Pi) limitation. These enzymes are thought scavenge Pi from organophosphate compounds in the rhizosphere and thus increase availability when deficient. The tight association APase with root surface may make more efficient utilization soil around tissues, which present forms. To date, however, no systematic molecular, biochemical, functional studies have been reported for any...
Abstract The phytohormone abscisic acid (ABA) is well known for its regulatory roles in integrating environmental constraints with the developmental programs of plants. Here, we characterize biological function Arabidopsis (Arabidopsis thaliana) RING-H2 protein RHA2a ABA signaling. rha2a mutant less sensitive to than wild type during seed germination and early seedling development, whereas transgenic plants overexpressing are hypersensitive, indicating that positively regulates ABA-mediated...
ABSTRACT Plant purple acid phosphatases (PAPs) belong to a large multigene family whose specific functions in Pi metabolism are poorly understood. Two PAP isozymes secreted by Pi‐deficient (−Pi) Arabidopsis thaliana were purified from culture filtrates of −Pi suspension cells. They correspond an AtPAP12 (At2g27190) homodimer and AtPAP26 (At5g34850) monomer composed glycosylated 60 55 kDa subunit(s), respectively. Each exhibited broad pH activity profiles centred at 5.6, overlapping substrate...
The large and complex hexaploid genome has greatly hindered genomics studies of common wheat (Triticum aestivum, AABBDD). Here, we investigated transcripts in developing caryopses using the emerging single-molecule real-time (SMRT) sequencing technology PacBio RSII, assessed resultant data for improving annotation grain transcriptome research. We obtained 197,709 full-length non-chimeric (FLNC) reads, 74.6 % which were estimated to carry complete open reading frame. A total 91,881...
NPR1 (NONEXPRESSER OF PR GENES1) functions as a master regulator of the plant hormone salicylic acid (SA) signaling and plays an essential role in immunity. In nucleus, interacts with transcription factors to induce expression (PATHOGENESIS-RELATED) genes thereby promote defense responses. However, underlying molecular mechanism gene activation is poorly understood. Furthermore, despite importance immunity, regulation has not been extensively studied. Here, we show that SA promotes...
Background:The continued success of oncological therapeutics is dependent on the mitigation treatment-related adverse events, particularly cardiovascular toxicities.As such, there an important need to understand basic mechanisms drug toxicities in process antitumor therapy.Our aim this study was elucidate underlying sorafenib (sor)-induced cardiomyocyte damage.Methods: Primary mouse cardiomyocytes were prepared and treated with sor various other treatments.Cardiomyocyte necroptosis detected...
Polyploidization and introgression are major events driving plant genome evolution influencing crop breeding. However, the mechanisms underlying higher-order chromatin organization of subgenomes alien chromosomes largely unknown.
The phytohormone salicylic acid (SA) plays a pivotal role in plant defense against biotrophic and hemibiotrophic pathogens. NPR1 EDS1 function as two central hubs local systemic immunity. However, it is unclear how orchestrates gene regulation whether directly participates transcriptional reprogramming. Here, we show that synergistically activate pathogenesis-related (PR) genes defenses by forming protein complex recruiting Mediator. We discover functions an autonomous coactivator with...
ABSTRACT Achieving high yield and good quality in crops is essential for human food security health. However, there usually disharmony between quality. Seed storage protein (SSP) starch, the predominant components cereal grains, determine quality, their coupled synthesis causes a yield–quality trade‐off. Therefore, dissection of underlying regulatory mechanism facilitates simultaneous improvement Here, we summarize current findings about synergistic molecular machinery underpinning SSP...