A5100422004- CRISPR and Genetic Engineering
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Plant Virus Research Studies
- Enzyme Catalysis and Immobilization
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Bacteriophages and microbial interactions
- Microbial Metabolites in Food Biotechnology
- Plant and Fungal Interactions Research
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Insect Resistance and Genetics
- Molecular Biology Techniques and Applications
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- GNSS positioning and interference
- Folate and B Vitamins Research
- Plant tissue culture and regeneration
- RNA regulation and disease
- Microbial Inactivation Methods
- Plant Disease Resistance and Genetics
- Ion Transport and Channel Regulation
Institute of Genetics and Developmental Biology
2008-2025
Chinese Academy of Sciences
2016-2025
Nanjing Forestry University
2018-2025
Shanghai Institute of Materia Medica
2021-2025
Research Center for Eco-Environmental Sciences
2025
University of Chinese Academy of Sciences
2022-2025
Chinese Academy of Forestry
2021-2025
Shanghai University of Engineering Science
2023-2025
Yunnan Normal University
2014-2024
Tsinghua University
2024
Nucleotide base editors in plants have been limited to conversion of cytosine thymine. Here, we describe a new plant adenine editor based on an evolved tRNA adenosine deaminase fused the nickase CRISPR/Cas9, enabling A•T G•C at frequencies up 7.5% protoplasts and 59.1% regenerated rice wheat plants. An endogenous gene is also successfully modified through introducing gain-of-function point mutation directly produce herbicide-tolerant plant. With this editing system, it now possible precisely...
Abstract Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry ( Fragaria vesca ), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content homozygous T1 mutant lines 33.9–83.6% higher than that wild-type. also recover a series transgene-free mutants with 35 genotypes containing continuum sugar content. All could be immediately fixed subsequent...
The genetic diversity and phenotypic variability of crop agronomic traits is valued by breeders for their benefits in breeding but are limited most target traits. Genome editing has proved to be a powerful tool quick efficient creation continuous beneficial variation (Eshed Lippman, 2019). rice Waxy (Wx) gene (LOC_Os06g04200) encodes granule-bound starch synthase I (GBSSI), which determines the amylose content (AC) endosperm controlling synthesis. This one major contributors eating cooking...
CRISPR-Cas12a (Cpf1) is widely used for pathogen detection. However, most Cas12a nucleic acid detection methods are limited by a PAM sequence requirement. Moreover, preamplification and cleavage separate. Here, we developed one-step RPA-CRISPR (ORCD) system unrestricted the with high sensitivity specificity that offers one-tube, rapid, visually observable of acids. In this system, RPA amplification performed simultaneously, without separate product transfer steps, 0.2 copies/μL DNA 0.4 RNA...
Here we report a biological and molecular characterization of novel positive-sense RNA virus isolated from field isolate (NW10) filamentous phytopathogenic fungus, the white root rot fungus that is designated as Rosellinia necatrix fusarivirus 1 (RnFV1). A recently developed technology using zinc ions allowed us to transfer RnFV1 two mycelially incompatible strains. comparison virus-free -recipient isogenic fungal strains suggested infects latently thus has no potential virocontrol agent....
Abstract The plant tryptophan (Trp) biosynthetic pathway produces many secondary metabolites with diverse functions. Indole‐3‐acetic acid (IAA), proposed as a derivative from Trp or its precursors, plays an essential role in growth and development. Although the Trp‐dependant Trp‐independent IAA pathways have been proposed, enzymes, reactions regulatory mechanisms are largely unknown. In Arabidopsis , indole‐3‐glycerol phosphate (IGP) is suggested to serve branchpoint component biosynthesis....
During the growth and development of land plants, some specialized cells, such as tracheary elements, undergo secondary cell wall thickening. Secondary walls contain additional lignin, compared with primary walls, thus providing mechanical strength potentially improving defenses against pathogens. However, molecular mechanisms that initiate thickening are unknown. In this study, we identified an Arabidopsis (Arabidopsis thaliana) leucine-rich repeat receptor-like kinase, encoded by AtVRLK1...
Abstract Background Upland cotton ( Gossypium hirsutum ), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic functional analyses extremely challenging. The recent accessibility CRISPR/Cas9 tool provides the ability to modify targeted locus efficiently in various complicated plant genomes. However, current transformation method targeting one requires complicated, long laborious...
Zellweger spectrum disorder (ZSD) is a heterogeneous group of diseases with high morbidity and mortality caused by failure to assemble normal peroxisomes. There no therapy for ZSD, but management supportive. Nevertheless, one-half the patients have phenotype milder than classic syndrome exhibit progressive disease course. Thus, would benefit if therapies became available were instituted early. Recent reports indicate several interventions that result in partial peroxisome recovery ZSD...