A5103203653- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- melanin and skin pigmentation
- Biochemical Analysis and Sensing Techniques
- Exercise and Physiological Responses
- Endoplasmic Reticulum Stress and Disease
- Cardiovascular Disease and Adiposity
- RNA modifications and cancer
- Hybrid Renewable Energy Systems
- Plant Genetic and Mutation Studies
- Hearing, Cochlea, Tinnitus, Genetics
- Acne and Rosacea Treatments and Effects
- Autoimmune and Inflammatory Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Dietary Effects on Health
- Microbial Metabolism and Applications
- Ocular Disorders and Treatments
- Muscle Physiology and Disorders
- Cancer-related gene regulation
- Regulation of Appetite and Obesity
- Hidradenitis Suppurativa and Treatments
- Phytase and its Applications
University of Michigan
2025
Chinese Academy of Sciences
2006-2023
Institute of Zoology
2015-2023
Laboratoire d’immunologie intégrative du cancer
2022
University of Chinese Academy of Sciences
2015-2021
State Key Laboratory of Stem Cell and Reproductive Biology
2019
Center for Excellence in Molecular Cell Science
2018
China Academy of Chinese Medical Sciences
2018
Wuhan Institute of Physics and Mathematics
2006
Significance Uncoupling protein 1 (UCP1) is responsible for brown adipose tissue-mediated thermogenesis and plays a critical role in protecting against cold regulating energy homeostasis. Modern pigs lack functional UCP1, which makes them susceptible to prone fat deposition results neonatal mortality decreased production efficiency. In the current study, CRISPR/Cas9-mediated homologous recombination-independent approach was established, mouse adiponectin-UCP1 efficiently inserted into...
Abstract Pig shows multiple superior characteristics in anatomy, physiology, and genome that have made this species to be more suitable models for human diseases, especially neurodegenerative because they similar cerebral convolutions compared with neocortex. Recently, CRISPR/Cas9 system enormous potential engineering the pig genome. In study, we expect generate Parkinson’s disease model using by simultaneously targeting three distinct genomic loci, parkin/DJ-1/ PINK1 , Bama miniature pigs....
Pigs lack functional uncoupling protein 1 (UCP1) making them susceptible to cold. Nevertheless, several pig breeds are known be cold resistant. The molecular mechanism(s) enabling such adaptation currently unknown. Here, we show that this resistance is not dependent on shivering, but rather depends UCP3 and white adipose tissue (WAT) browning. In two cold-resistant (Tibetan Min), a cold-sensitive breed (Bama), WAT browning was induced after exposure. Beige adipocytes from Tibetan pigs...
Abstract Genetic engineering in livestock was greatly enhanced by the emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), which can be programmed with a single-guide RNA (sgRNA) to generate site-specific DNA breaks. However, uncertainties caused wide variations sgRNA activity impede utility this system generating genetically modified pigs. Here, we described single blastocyst genotyping provide simple and rapid solution evaluate compare...
Accumulating evidence suggests that faulty epigenetic reprogramming leads to the abnormal development of cloned embryos and results in low success rates observed all mammals produced through somatic cell nuclear transfer (SCNT). The aberrant methylation status H3K9me H3K9me2 has been reported mouse embryos. To explore role porcine reprogramming, BIX-01294, known as a specific inhibitor G9A (histone-lysine methyltransferase H3K9), was used treat nuclear-transferred (NT) oocytes for 14-16 h...
Mitochondrial uncoupling protein 1 (UCP1) is the hallmark of brown adipocytes responsible for cold- and diet-induced thermogenesis. Here, we report a previously unidentified role UCP1 in maintaining vascular health through its anti-inflammatory actions possibly perivascular adipose tissue. deficiency exacerbates dietary obesity-induced endothelial dysfunction, inflammation, atherogenesis mice, which was not rectified by reconstitution interscapular Mechanistically, lack augments...
N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on large scale efficiently, and discover genes with novel functions at the whole-genome level in Caenorhabditis elegans, flies, zebrafish mice, but it has never been tried model animals. We describe successful systematic three-generation ENU screening pigs establishment of Chinese Swine Mutagenesis Consortium. A total 6,770 G1 6,800 G3 were screened, 36 dominant 91 recessive pig families various phenotypes...
The relationship between the host gut microbiota and obesity has been well documented in humans mice; however, few studies reported association fat deposition pigs. In a previous study, we generated uncoupling protein 1 (UCP1) knock-in pigs (UCP1 pigs), which exhibited lower phenotype. Whether was reshaped these whether contributes to content remain unknown. Here, revealed that fecal composition metabolites were significantly altered under both chow diet (CD) high-fat/high-cholesterol (HFHC)...
Abstract METTL14 dimerizes with METTL3 to install N6‐methyladenosine (m 6 A) on mRNA A writers). Subsequently, m readers bind A‐marked RNA influence its metabolism. emerges critically regulate multiple intracellular processes; however, there is a gap in our understanding of liver Glucose‐6‐phosphatase catalytic subunit (G6pc) mediates hepatic glucose production (HGP) and serves as the gatekeeper for glycogenolysis gluconeogenesis; G6pc regulation not fully understood. Here, identified...
Pig is an important agricultural economic animal, providing large amount of meat products. With the development functional genomics and bioinformatics, lots genes single nucleotide polymorphism (SNPs) related to disease resistance (or) traits in pigs have been identified which provides targets for genetic improvement by genome editing. Base editors (BEs), combining Cas9 nickase cytidine or adenine deaminase, achieve all four possible transition mutations (C T, A G, T C G A) efficiently...
Research Article16 November 2018Open Access Source DataTransparent process Rescuing ocular development in an anophthalmic pig by blastocyst complementation Hongyong Zhang State Key Laboratory of Stem Cell and Reproductive Biology, Institute Zoology, Chinese Academy Sciences, Beijing, China Savaid Medical School, University Regeneration, Search for more papers this author Jiaojiao Huang Zechen Li College Life Qufu Normal University, Qufu, Guosong Qin Nan Tang Hai Qianlong Hong Qiantao Zheng...
Abstract Harlequin ichthyosis (HI) is a severe genetic skin disorder and caused by mutation in the ATP-binding cassette A12 (ABCA12) gene. The retinoid administration has dramatically improved long-term survival of HI, but improvements are still needed. However, ABCA12 null mice failed to respond treatment, which impedes development novel cure strategies for HI. Here we generated an ethylnitrosourea mutagenic HI pig model (named Z9), carries deep intronic IVS49-727 A>G gene, resulting...
Metabolic dysfunction-associated steatotic liver disease (MASLD) is characterized by excessive hepatic lipid accumulation and can progress to metabolic steatohepatitis (MASH), which manifested with persistent injury, inflammation, fibrosis, increasing the risk for cirrhosis hepatocellular carcinoma. Aberrant epigenetic reprogramming epitranscriptomic remodeling emerge be a driving force MASLD MASH. SNAIL1 SLUG, two related transcriptional regulators, regulate de novo lipogenesis steatosis...
Leptin exerts its biological actions by activating the long-form leptin receptor (LepRb). LepRb signaling impairment and resistance are believed to cause obesity. The transcription factor Slug — also known as Snai2 recruits epigenetic modifiers regulates gene expression an mechanism; however, action has not been explored in resistance. Here, we uncover a proobesity function of neuronal Slug. Hypothalamic was upregulated obese mice. LepRb+ cell–specific Slug-knockout (SlugΔLepRb) mice were...
ABSTRACT Pigs share many similarities with humans in terms of anatomy, physiology and genetics, have long been recognized as important experimental animals biomedical research. Using an N-ethyl-N-nitrosourea (ENU) mutagenesis screen, we previously identified a large number pig mutants, which could be further established human disease models. However, the identification causative mutations great heterogeneity remains challenging endeavor. Here, select one mutant, showing congenital nude skin...
The photodissociation dynamics of C8H17Br with excitation laser at 234 nm has been studied using velocity map ion imaging. relative quantum yields are obtained from (2+1) resonance-enhanced multiphoton ionization (REMPI) the photo-fragment Br*(2P1/2) and Br(2P3/2). maps provide detailed information on translational energy distributions angle distributions. probabilities individual pathways fragments calculated yield angular distribution. results indicate that a large fraction available...
Abstract Precise and simultaneous acquisition of multiple beneficial alleles in the genome to improve pig performance are pivotal for making elite breeders. Cytidine base editors (CBEs) have emerged as powerful tools site-specific single nucleotide replacement. Here, we compare editing efficiency four CBEs porcine embryonic cells embryos show that hA3A-BE3-Y130F hA3A-eBE3-Y130F consistently results higher base-editing lower toxic effects vitro embryo development. We also zygote...