A5101939004- Animal Genetics and Reproduction
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- Pancreatic function and diabetes
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- CRISPR and Genetic Engineering
- Muscle Physiology and Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Ovarian function and disorders
- Liver Disease Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Xenotransplantation and immune response
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Mesenchymal stem cell research
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
Chinese Academy of Agricultural Sciences
2016-2025
The First Affiliated Hospital, Sun Yat-sen University
2025
Sun Yat-sen University
2025
Tongji Hospital
2015-2024
Huazhong University of Science and Technology
2003-2024
Sichuan University
2024
Zhejiang University
2024
West China Hospital of Sichuan University
2024
National Institute for Parasitic Diseases
2024
Northwest University
2023
Background:The pig is an economically important food source, amounting to approximately 40% of all meat consumed worldwide. Pigs also serve as model organism because their similarity humans at the anatomical, physiological and genetic level, making them very useful for studying a variety human diseases. A strain particular interest miniature pig, specifically Wuzhishan (WZSP), it has been extensively inbred. Its high level homozygosity offers increased ease selective breeding specific traits...
Abstract Transgenic pigs play an important role in producing higher quality food agriculture and improving human health when used as animal models for various diseases biomedicine. Production of transgenic pigs, however, is a lengthy inefficient process that hinders research using pig models. Recent applications the CRISPR/Cas9 system generating site-specific gene knockout/knockin models, including knockout model, have significantly accelerated model field. However, knockin containing...
Long intergenic non-coding RNAs (lincRNAs) play important roles in many cellular processes. Here, we present the first systematic identification and characterization of lincRNAs fetal porcine skeletal muscle. We obtained a total 55.02 million 90-bp paired-end reads assembled 54,550 transcripts using cufflinks. developed pipeline to identify 570 multi-exon by integrating set previous approaches. These putative share characteristics with mammalian lincRNAs, such as relatively short length,...
Chinese indigenous pig breeds are recognized as an invaluable component of the world's genetic resources and divided traditionally into six types. Twenty-six microsatellite markers recommended by FAO (Food Agriculture Organization) ISAG (International Society Animal Genetics) were employed to analyze diversity 18 with 1001 individuals representing five types, three commercial 184 individuals. The observed heterozygosity, unbiased expected heterozygosity effective number alleles used estimate...
Metabolic syndrome can induce chronic renal injury in humans. In the present study, Bama minipigs were fed a high-fat/high-sucrose diet (HFHSD) for 23 months, which caused them to develop pathological characteristics of metabolic syndrome, including obesity, hyperinsulinemia, and hyperlipidemia, resulted kidney tissue damage. HFHSD group, ratio glomus areas glomerulus area glomerular density inside cortex both decreased. Lipid deposition tubules was detected up-regulated expression levels...
Abstract: Sepsis is a systemic inflammatory response syndrome and mainly caused by lipopolysaccharides (LPS) – component of the cell walls gram-negative bacteria, via toll-like receptor 4–mitogen-activated protein kinases/nuclear factor-kappa B-dependent proinflammatory signaling pathway. Here, we synthesized 26 asymmetric monocarbonyl analogs curcumin evaluated their anti-inflammatory activity inhibiting LPS-induced secretion tumor necrosis factor-α interleukin-6 in mouse RAW264.7...
To systematically evaluate the associations between glycemic control and short- to long-term outcomes in coronavirus disease 2019 (COVID-19) patients with type 2 diabetes (T2D).A multi-center prospective cohort study including 574 COVID-19 T2D was conducted Wuhan, China. All were followed-up 1 year after hospital discharge using a uniformed questionnaire self-reported symptoms, chronic obstructive pulmonary assessment test items.Of patients, 443 (77.2%) had well-controlled blood glucose....
Abstract The minipig can serve as a good pharmacological model for human subjects. However, the long-term pathogenesis of high-calorie diet-induced metabolic syndromes, including NASH, has not been well described in minipigs. We examined development syndromes Bama minipigs that were fed high-fat, high-sucrose diet (HFHSD) 23 months, by using histology and serum biochemistry profiling gene expression patterns livers HFHSD pigs compared to controls. pathology findings revealed microvesicular...
The mechanisms of diabetic renal injury remain unclear. Recent studies have shown that immunological and inflammatory elements play important roles in the initiation development nephropathy (DN). Toll-like receptors (TLRs) comprise a superfamily innate immune system receptors. TLRs pathogenesis lesions are mostly unknown. Compared with rodents, miniature pigs more similar to humans respect metabolism, kidney structure, system, therefore represent an ideal large-animal model for DN...
Abstract Today, obesity and nonalcoholic steatohepatitis are a worldwide epidemic, although how these syndromes regulated with respect to lncRNAs remains largely unknown. Our previous studies have revealed important pathological features molecular characteristics of in the minipig model, this study, we analyze their potential target genes. Minipig samples only from liver were analyzed using next-generation deep sequencing. In total, obtained 585 million raw reads approximately 70.4 Gb high...
N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on large scale efficiently, and discover genes with novel functions at the whole-genome level in Caenorhabditis elegans, flies, zebrafish mice, but it has never been tried model animals. We describe successful systematic three-generation ENU screening pigs establishment of Chinese Swine Mutagenesis Consortium. A total 6,770 G1 6,800 G3 were screened, 36 dominant 91 recessive pig families various phenotypes...
Breast cancer is the most frequently diagnosed among women, and circulating tumor cell (CTC)-meditated distant metastasis leading cause of death. Thus, detection CTCs great importance for early diagnosis breast prevention metastasis. In this study, using human carcinoma BT474 cells as model CTCs, a powerful assay platform demonstrated by fluorescence spectrometry highly sensitive CTC combining dual-recognizing elements receptor-binding antibody aptamer-mediated separation with double rolling...
To evaluate the accuracy and clinical effect of immediate implant placement(IIP) using real-time dynamic navigation in posterior maxilla with alveolar bone defects. A total 55 patients 72 implants placed maxillary region defects were retrospectively analyzed between January 1, 2021, October 31, 2024. The study was divided into two groups, group freehand group.The preoperative planning data postoperative CBCT actual imported verification software, deviations neck, root, depth angle calculated...
This research aims to reveal the cellular cross talk in fibrosis liver using transgenic pigs (TG) expressing humanized risk genes (PNPLA3I148M-GIPRdn-hIAPP) as a metabolic dysfunction-associated steatotic disease (MASLD) model. The study uses single-nucleus sequencing differentiation and interaction characteristics of various cell populations during development MASLD. After 6 months high-fat, high-sucrose diet induction, model exhibited obvious pathological features, including fat...
There are conflicting reports on the link between micronutrient selenium and prevalence of diabetes. To investigate possibility that acts as a "double-edged sword" in diabetes, cDNA microarray profiling two-dimensional differential gel electrophoresis coupled with mass spectrometry were used to determine changes mRNA protein expression pancreatic liver tissues diabetic db/db mice response dietary selenate supplementation. Fasting blood glucose levels increased continuously administered...
Mesenchymal stem cells (MSCs) have the ability to proliferate in vivo with a large variety of differentiation potentials and therefore are widely used as an ideal material for cell therapy. MSCs derived from pig human sources similar many aspects, such immunophenotype functional characteristics. However, differences proteomics molecular mechanisms functions between porcine bone marrow (BM-MSCs) umbilical cord (UC-MSCs) largely unknown. To best our knowledge, collected different tissue...
A long-term high-energy diet affects human health and leads to obesity metabolic syndrome in addition cardiac steatosis hypertrophy. Ectopic fat accumulation the heart has been demonstrated be a risk factor for disorders, but molecular mechanism of disease remains largely unknown. Bama miniature pigs were fed high-fat, high-sucrose (HFHSD) 23 months. These developed symptoms showed hypertrophy with greatly increased body weight (2.73-fold, P<0.01), insulin level (4.60-fold, (1.82-fold,...
Abstract: A novel class of asymmetric mono-carbonyl analogs curcumin (AMACs) were synthesized and screened for anti-inflammatory activity. These are chemically stable as characterized by UV absorption spectra. In vitro, compounds 3f , 3m 4b 4d markedly inhibited lipopolysaccharide (LPS)-induced expression pro-inflammatory cytokines tumor necrosis factor-α interleukin-6 in a dose-dependent manner, with IC 50 values low micromolar range. vivo, compound demonstrated potent preventive...
Long-term adherence to a high-fat, high-calorie diet influences human health and causes obesity, metabolic syndrome nonalcoholic steatohepatitis (NASH). Inflammation plays key role in the development of NASH; however, mechanism inflammation induced by over-nutrition remains largely unknown. In this study, we fed Bama minipigs high-sucrose (HFHSD) for 23 months. The pigs exhibited characteristics developed with greatly increased numbers inflammatory cells, such as lymphocytes (2.27-fold,...
Objectives The effect of a long-term high-fat, high-caloric diet on the dysfunction pancreas has not been clarified. We investigated pancreatic histopathology and β-cell apoptosis in Bama minipigs after 23 months high-fat high-sucrose (HFHSD). Methods were randomly assigned to control (n = 6) HFHSD groups for months, biochemical parameters measured. Pancreata subjected histological analysis, followed by assessment with transmission electron microscopy. Lipid peroxidation was determined...
Electrochemical DNA (E-DNA) sensors have been greatly developed and play an important role in early diagnosis of different diseases. To determine the extremely low abundance biomarkers clinical samples, scientists are making unremitting efforts toward achieving highly sensitive selective E-DNA sensors. Here, a novel sensor was taking advantage signal amplification efficiency nanoprobe-initiated enzymatic polymerization (NIEP). In NIEP based sensor, capture probe thiolated at its 3'-terminal...
Anti-inflammatory therapies have the potential to become an effective treatment for obesity-related diseases. However, huge gap of immune system between human and rodent leads limitations drug discovery. This work aims at constructing a transgenic pig model with higher risk metabolic diseases outlining responses early stage metaflammation by transcriptomic strategy. We used CRISPR/Cas9 techniques targeted knock-in three humanized disease genes, GIPR dn , hIAPP PNPLA3 I148M . Transgenic...
Bone morphogenetic protein 2 (BMP2) has been reported to regulate adipogenesis, but its role in porcine beige adipocyte formation remains unclear. Our data reveal that BMP2 is significantly induced at the early stages of differentiation. Additionally, supplementing rhBMP2 during stages, not late differentiation, enhances SVF thermogenesis, and proliferation. Furthermore, compared empty plasmid-transfected-SVFs, BMP2-overexpressed SVFs had enhanced lipid accumulation while knockdown exhibited...
DNA pooling can provide an economic and efficient way to detect susceptibility loci complex diseases. We carried out a genome screen with 400 microsatellite markers spaced at approximately 10 cm in two pools consisting of 119 schizophrenia (SZ) patients controls recruited from homogenous population the Chang Le area Shandong peninsula China. Association D6S289, dinucleotide repeat polymorphism JARID2 gene SZ, was found confirmed by individual genotyping ( X 2 = 17.89; P .047). In order...