A5067416656- Hippo pathway signaling and YAP/TAZ
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Viral gastroenteritis research and epidemiology
- Plant nutrient uptake and metabolism
- Mass Spectrometry Techniques and Applications
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Drug Transport and Resistance Mechanisms
- Advanced Proteomics Techniques and Applications
- Plant and Fungal Interactions Research
- Influenza Virus Research Studies
- Cell death mechanisms and regulation
- Plant biochemistry and biosynthesis
- Trace Elements in Health
- Plant Stress Responses and Tolerance
- Polysaccharides and Plant Cell Walls
- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
- Bacterial Genetics and Biotechnology
- Platelet Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Escherichia coli research studies
Health Canada
2011-2024
Sun Yat-sen University Cancer Center
2023
Sun Yat-sen University
2023
Chinese Academy of Sciences
2013-2022
Center for Excellence in Molecular Plant Sciences
2014-2022
Tianjin Beichen Hospital
2017
Shanghai Institutes for Biological Sciences
2016
Fujian Blood Center
2015
Songjiang District Central Hospital
2014
Queen's University
2007-2013
ABSTRACT Plant purple acid phosphatases (PAPs) belong to a large multigene family whose specific functions in Pi metabolism are poorly understood. Two PAP isozymes secreted by Pi‐deficient (−Pi) Arabidopsis thaliana were purified from culture filtrates of −Pi suspension cells. They correspond an AtPAP12 (At2g27190) homodimer and AtPAP26 (At5g34850) monomer composed glycosylated 60 55 kDa subunit(s), respectively. Each exhibited broad pH activity profiles centred at 5.6, overlapping substrate...
The large tumor suppressor 1 (LATS1) is a serine/threonine kinase and found down-regulated in broad spectrum of human cancers. LATS1 central player the emerging Hippo-LATS pathway, which plays important roles cell proliferation, apoptosis, stem differentiation. Despite ample data supporting role for suppression, how regulated at molecular level remains largely unknown. In this study, we have identified Itch, HECT class E3 ubiquitin ligase, as unique binding partner LATS1. Itch can complex...
Elevated temperatures limit plant growth and reproduction pose a growing threat to agriculture. Plant heat stress response is highly conserved fine-tuned in multiple pathways. Spinach (Spinacia oleracea L.) cold tolerant but sensitive green leafy vegetable. In this study, adaptation mechanisms spinach sibling inbred heat-tolerant line Sp75 were investigated using physiological, proteomic, phosphoproteomic approaches. The abundance patterns of 911 stress-responsive proteins, phosphorylation...
Abstract Platelet αIIbβ3 integrin and its ligands are essential for thrombosis hemostasis, play key roles in myocardial infarction stroke. Here we show that apolipoprotein A-IV (apoA-IV) can be isolated from human blood plasma using platelet β3 integrin-coated beads. Binding of apoA-IV to platelets requires activation integrin, the direct apoA-IV-αIIbβ3 interaction detected a single-molecule Biomembrane Force Probe. We identify aspartic acids 5 13 at N-terminus required binding which is...
PEPC [PEP(phosphoenolpyruvate) carboxylase] is a tightly controlled cytosolic enzyme situated at major branchpoint in plant metabolism. Accumulating evidence indicates important functions for and PPCK (PEPC kinase) acclimation to nutritional Pi deprivation. However, little known about the genetic origin or phosphorylation status of native PEPCs from −Pi (Pi-deficient) plants. The transfer Arabidopsis suspension cells seedlings growth media resulted in: (i) marked transcriptional upregulation...
Abstract Although antitubulin drugs are used widely to treat human cancer, many patients display intrinsic or acquired drug resistance that imposes major obstacles successful therapy. Mounting evidence argues cancer cell apoptosis triggered by relies upon activation of the cell-cycle kinase Cdk1; however, mechanistic connections this event remain obscure. In study, we identified antiapoptotic protein YAP, a core component Hippo signaling pathway implicated in tumorigenesis, as critical...
Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 Escherichia coli wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 capsid Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 Gp77 (tail...
The subversion of plant cellular functions is essential for bacterial pathogens to proliferate in host plants and cause disease. Most employ a type III secretion system inject effector (T3E) proteins inside cells, where they contribute the pathogen-induced alteration physiology. In this work, we found that Ralstonia solanacearum T3E RipAY suppresses immune responses triggered by elicitors phytohormone salicylic acid. Further biochemical analysis indicated associates planta with thioredoxins...
Plant purple acid phosphatases (PAPs) belong to a relatively large gene family whose individual functions are poorly understood. Three PAP isozymes that up-regulated in the cell walls of phosphate (Pi)-starved (-Pi) Arabidopsis thaliana suspension cells were purified and identified by MS as AtPAP12 (At2g27190), AtPAP25 (At4g36350) AtPAP26 (At5g34850). previously isolated from culture medium -Pi cultures, shown be secreted roots seedlings facilitate Pi scavenging soil-localized...
Summary N‐glycosylation has a great impact on glycoprotein structure, conformation, stability, solubility, immunogenicity and enzyme activity. Structural characterization of N‐glycoproteome been challenging but can provide insights into the extent protein folding surface topology. We describe highly sensitive proteomics method for large‐scale identification quantification glycoproteins in Arabidopsis through 15 N‐metabolic labeling, selective enrichment glycopeptides, data‐dependent MS /...
The metalloproteome is defined as the set of proteins that have metal-binding capacity by being metalloproteins or having sites. A different may exist for each metal. Mass spectrometric characterization metalloproteomes provides valuable information relating to cellular disposition metals physiologically and in metal-associated diseases. We examined Cu Zn three human hepatoma lines: Hep G2 Mz-Hep-1, which retain many functional characteristics normal hepatocytes, SK-Hep-1, poorly...
To further our knowledge of intracellular copper transport, we used a proteomics strategy to search for hepatic proteins with copper-binding ability. Hep G2 cytosolic and microsomal fractions were applied copper(II)-loaded immobilized metal-affinity chromatography (IMAC) column. Protein identification was performed 2-D gel electrophoresis mass spectrometry. We identified 48 19 displaying These are diverse in function. Fifty-two the 67 contain putative metal-binding domains. have many...
Despite multiple control measures, Escherichia coli O157:H7 (STEC O157:H7) continues to be responsible for many food borne outbreaks in North America and elsewhere. Bacteriophage therapy may prove useful controlling this pathogen the host, their environment food. vB_EcoS_AKFV33 (AKFV33), a T5-like phage of Siphoviridae lysed common types STEC not non-O157 E. coli. Moreover, isolated from same feedlot pen which was obtained, were highly susceptible AKFV33. Adsorption rate constant burst size...
Abstract Background Comparatively little information is available on members of the Myoviridae infecting low G+C content, Gram-positive host bacteria family Firmicutes. While numerous Bacillus phages have been isolated up till now only very few cereus characterized in detail. Results Here we present data large, virulent, broad-host-range B. phage vB_BceM_Bc431v3 (Bc431v3). Bc431v3 features a 158,618 bp dsDNA genome, encompassing 239 putative open reading frames (ORFs) and, 20 tRNA genes...
Summary Small signalling peptides, generated from larger protein precursors, are important components to orchestrate various plant processes such as development and immune responses. However, small peptides involved in immunity remain largely unknown. Here, we developed a pipeline using transcriptomics‐ proteomics‐based screening identify putative precursors of peptides: secreted proteins ( SSP s) rice, induced by rice blast fungus Magnaporthe oryzae its elicitor, chitin. We identified 236 s...
Characterization of the structural diversity glycans by liquid chromatography–tandem mass spectrometry (LC–MS/MS) remains an analytical challenge in large-scale glycomics applications because presence heterogeneous composition, ubiquitous isomers, lability post-translational glycan modifications, and complexity data interpretation. High-resolution separation isomers differentiating from positional, linkage, branching, anomeric structures is often a prerequisite to ensure comprehensive...
Nedd4 (Nedd4-1) is a Hect domain E3 ubiquitin ligase that also contains C2 and three WW domains. Despite numerous in vitro studies, its biological function vivo not well understood. Here we show disruption of Nedd4-1 mice (leaving Nedd4-2 intact) caused embryonic lethality at mid gestation, with pronounced heart defects (double-outlet right ventricle atrioventricular cushion defects) vasculature abnormalities. Quantitative mass spectrometry immunoblot analyses lysates from the wild type...
Phosphoenolpyruvate carboxylase (PEPC) is a tightly regulated enzyme situated at the core of plant C-metabolism. Although its anaplerotic role and control by allosteric effectors, reversible phosphorylation, oligomerization have been well documented in endosperm developing castor oil seeds (COS), relatively little known about PEPC germinating COS. The initial phase COS germination was accompanied elevated activity accumulation comparable amounts pre-existing 107-kDa inducible 110-kDa...
Lytic bacteriophages have been applied successfully to control the growth of various foodborne pathogens. Sequencing their genomes is considered as an important preliminary step ensure safety prior food applications. The lytic bacteriophage, ΦSboM-AG3, targets pathogen, Shigella. It morphologically similar phage ViI Salmonella enterica serovar Typhi and a series phages Acinetobacter calcoaceticus Rhizobium meliloti. complete genome ΦSboM-AG3 was determined be 158 kb terminally redundant...
Inactivation of intact influenza viruses using formaldehyde or β-propiolactone (BPL) is essential for vaccine production and safety. The extent chemical modifications such reagents on viral proteins needs to be extensively investigated better control the reactions quality vaccines. We have evaluated effect BPL inactivation two candidate re-assortant vaccines (NIBRG-121xp NYMC-X181A) derived from A/California/07/2009 pandemic high-resolution FT-ICR MS-based proteomic approaches. report here...
A potyvirus (proposed name of Zea mosaic virus [ZeMV]) isolated from maize in Israel was analyzed by serology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) capsid proteins, symptomatology, and sequencing. Parts the nuclear inclusion b, coat protein, 3′ regions were sequenced; amino acid sequence ZeMV determined time-of-flight mass spectrometry (TOFMS). The results these analyses compared with those similar following potyviruses: Maize dwarf (MDMV), Sugarcane strain...