A5045189776- Enzyme Structure and Function
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Conducting polymers and applications
- Advanced Sensor and Energy Harvesting Materials
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Chromatography in Natural Products
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Magnetism in coordination complexes
- Computational Drug Discovery Methods
- Genetics and Neurodevelopmental Disorders
- Crystallography and molecular interactions
- Cancer-related gene regulation
Beijing Normal University
2016-2025
Beijing Technology and Business University
2019-2020
Queen's University
2005-2018
Weihai Science and Technology Bureau
2015
Nankai University
2006-2007
Chinese Academy of Sciences
2000
The development of capacitive pressure sensors with low cost, high sensitivity and facile fabrication techniques is desirable for flexible electronics wearable devices. In this project, a highly sensitive sensor was fabricated by sandwiching porous PAP sponge dielectric layer between two copper electrodes. introducing conductive silver nanowires (AgNWs) into the PDMS 100% sucrose as template polydimethylsiloxane (PDMS) film coating surface. optimized increasing load amount AgNWs....
In this work, we fabricated a high-performance and biocompatible porous triboelectric nanogenerator (TENG) with friction layers made by silk fibroin (SF)@MXene composite aerogel (SF@MXene-A) PDMS sponge. The incorporation of MXene into the SF increases specific surface area as well resulting charge density, providing way for designing TENG. TENG achieves an optimum output performance at ratio to about 1:1, obtaining maximum open-circuit voltage (Voc) 545 V short-circuit current (Isc) 16.13...
This study presents the fabrication of an ultralight, porous, and high-performance triboelectric nanogenerator (TENG) utilizing silk fibroin (SF) aerogels PDMS sponges as friction layer. The transition from two-dimensional film layers to three-dimensional porous significantly increased specific surface area, offering effective strategy for designing SF aerogel-based TENGs. TENG incorporating aerogel exhibited optimal output performance at a 3% concentration, achieving maximum open circuit...
. A mutant (Serl39Ala) of the mouse recombinant catalytic (C) subunit cAMP-dependent protein kinase was co-crystallized with a peptide inhibitor, PKI(5-24), and MEGA-8 (octanoyl-N-methylglucamide) detergent. This structure refined using all observed data (30 248 reflections) between 30 1.95 resolution to an R factor 0.186. R.m.s. deviations bond lengths angles are 0.013 2.3 degrees, respectively. The final model has 3075 atoms (207 solvent) mean B 31.9 A(2). placement invariant...
Abstract The activity of the protein phosphatase calcineurin (CN) is regulated by an autoinhibition mechanism wherein several domains from its catalytic A subunit, including calmodulin binding domain (CaMBD), block access to active site. Upon Ca 2+ and (Ca /CaM) CaMBD, autoinhibitory dissociate groove, thus activating enzyme. To date, structure CN/CaM/Ca complex has not been determined in entirety. Previously, we a fusion consisting CaM 25‐residue peptide taken joined 5‐glycine linker. This...
Summary The Cpx signalling system of Escherichia coli and Salmonella enterica senses extracytoplasmic stress controls expression factors that allow the bacterium to adapt these stressors thereby enhance survival. Many Cpx‐responsive genes products are unknown function. We determined crystal structure one gene products, called YihE in E. , which exhibits a eukaryotic kinase fold. Functional assays established both S. homologue, RdoA, undergo autophosphorylation phosphorylate protein...
This study investigates the roles of Fer-CIP4 homology (FCH)-Bin/amphiphysin/Rvs (F-BAR) and SH2 domains Fes protein tyrosine kinase in regulating its activation signaling downstream high-affinity immunoglobulin G (IgE) receptor (FcepsilonRI) mast cells. Homology modeling F-BAR domain revealed conservation some basic residues implicated phosphoinositide binding (R113/K114). The can bind phosphoinositides induce tubulation liposomes vitro. Mutation R113/K114 to uncharged (RK/QQ) caused a...
Inhibition of α-glucosidase has attracted the attention researchers due to its connection type-2 diabetes. Hydroxysafflor yellow A (HSYA) extracted from Carthamus tinctorius L. is a natural antioxidant used in traditional Chinese medicine. In this study, effect HSYA on was evaluated using inhibitory kinetics based properties and by performing computational simulation integration methods. reversibly inhibited competitive inhibition manner kinetic parameters were IC50 = 1.1 ± 0.22 mM Ki 1.04...
The two nucleotide binding domains (NBDs) of ATP cassette (ABC) transporters dimerize to form composite sites (NBSs) each containing Walker A and B motifs from one domain the ABC "C" signature other. In many proteins, NBSs are thought be functionally equivalent. However, this is not case for ABCC such as MRP1, in which NBS1 N-proximal NBD1 typically binds with high affinity but has low hydrolytic activity, while reverse true NBS2. notable feature proteins lack a catalytic Glu residue...
ABSTRACT MICU2 has been reported to interact with MICU1 and participate in the regulation of mitochondrial Ca2+ uptake, although molecular determinants underlying function is unknown. In order characterize we screened a series N-terminal C-terminal truncations obtained constructs which can be expressed abundance, giving rise soluble samples enable subsequent characterizations. Size exclusion chromatography (SEC) multi-angle laser light scattering (MALLS) revealed that exists as monomer...
Abstract Lanosterol is a potential drug for cataracts treatment, which can reverse the aggregation of intracrystalline proteins. The low concentration in lanolin calls high‐performance separation methods. In this study, counter‐current chromatography dual‐mode elution method was developed first time to separate and purify lanosterol from hexane extract after saponification, column eluted with lower phase as mobile head‐to‐tail mode, followed by upper tail‐to‐head mode. High purity...