A5102707973- Cancer-related molecular mechanisms research
- Ion channel regulation and function
- RNA modifications and cancer
- Neuroscience and Neuropharmacology Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Cardiac electrophysiology and arrhythmias
- Circular RNAs in diseases
- Ion Transport and Channel Regulation
- Fungal and yeast genetics research
- MicroRNA in disease regulation
- Gut microbiota and health
- Kruppel-like factors research
- Diet and metabolism studies
- Mycotoxins in Agriculture and Food
- Genetic Syndromes and Imprinting
- COVID-19 and Mental Health
- Receptor Mechanisms and Signaling
- Analytical Chemistry and Chromatography
- RNA Research and Splicing
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Stress Responses and Cortisol
- Crystallography and molecular interactions
- Metabolomics and Mass Spectrometry Studies
Shandong First Medical University
2024
Guangxi Medical University
2019-2024
Xinjiang University
2023-2024
Shanghai Center for Brain Science and Brain-Inspired Technology
2024
Shandong University
2016-2024
Ministry of Agriculture and Rural Affairs
2020-2022
Chinese Academy of Agricultural Sciences
2020-2022
Institute of Food Science and Technology
2020-2022
Shandong Xiehe University
2022
Institute of Agro-Products Processing Science and Technology
2022
The striatum is the most prominent nucleus in basal ganglia and plays an important role motor movement regulation. cholinergic interneurons (ChIs) are involved motion regulation by releasing acetylcholine (ACh) modulating output of striatal projection neurons. Here we report that muscarinic receptor (M receptor) agonists, ACh Oxotremorine (OXO-M), decreased firing frequency ChIs blocking hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. Scopolamine (SCO), a nonselective...
PTPN12 is an important tumor suppressor that plays critical roles in various physiological processes. However, the molecular basis underlying substrate specificity of remains uncertain. Here, enzymological and crystallographic studies have enabled us to identify two distinct structural features are crucial determinants specificity: pY+1 site binding pocket specific basic charged residues along its surface loops. Key structurally plastic regions recognition different HER2 phosphorylation...
TREK-1 is a member of the two-pore domain potassium channel family that known as leak and plays key role in many physiological pathological processes. The conformational transition selectivity filter considered an effective strategy for channels to control course efflux. It well regulated by large volume extracellular intracellular signals. However, until now, little was about gating mechanism channel. In this research, it found Ba(2+) blocked concentration- time-dependent manner. A...
Abstract TREK-2, a member of two-pore-domain potassium channel family, regulates cellular excitability in response to diverse stimuli. However, how such stimuli control function remains unclear. Here, by characterizing the responses cytosolic proximal C-terminus deletant (ΔpCt) and transmembrane segment 4 (M4)-glycine hinge mutant (G312A) 2-Aminoethoxydiphenyl borate (2-APB), an activator we show that transduction initiated from pCt domain is allosterically coupled with conformation...
Aim Liver‐specific non‐coding RNAs have been reported to play crucial roles in hepatocellular carcinoma (HCC). We investigated the possible biological performance of a novel liver‐specific long RNA, LINC02499, HCC. Methods The association between LINC02499 expression and HCC was evaluated based on Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases, then confirmed cohort by quantitative real‐time polymerase chain reaction. effects cells were verified gain‐ loss‐of‐function...
Aspergillus flavus is an important saprophytic fungus that produces aflatoxins (AF), which threaten food and feed safety. MAPK signaling pathways are essential for fungal adaptation to diverse environments.
In-depth studies on plant ion uptake and growth-promoting rhizobacteria (PGPR) at the molecular level will help to further reveal effects of PGPR plants their interaction mechanisms under salt stress.Cotton was inoculated with a PGPR-Enterobacter cloacae Rs-35, capacity, membrane transporter protein activity, expression key genes were determined stress. Changes in endogenous hormone content cotton also determined. Further, genome-wide metabolic pathway annotation E. Rs-35 its differential...
Abstract: Myocardial cell death during acute myocardial infarction occurs because of ischemia, persistent reperfusion-associated injury, and the inflammatory infiltrate as a response to necrosis. In present study, quantitative real-time PCR showed that lncRNA Gm4419 was highly upregulated in ischemia/reperfusion tissues hypoxia/reoxygenation H9C2 cells, whereas miR-682 downregulated. Knocking down with sh-Gm4419 resulted rescue apoptosis induced by or hypoxia/reoxygenation. Our study further...
Background: The outbreak of the novel coronavirus disease COVID-19 caused panic and psychological stress throughout World. We investigated extent adverse reactions in two medical staff groups China, explored importance online assistance for them. Methods: A cross-sectional survey including Hospital Anxiety Depression Scale (HADS) Insomnia Severity Index (ISI) was utilized to assess anxiety, depression, insomnia. Propensity score matching (PSM) applied match sex age between groups....
Metabolic study of bioactive compounds that undergo a dynamic and sequential process metabolism is still great challenge. Salidroside, one the most active ingredients Rhodiola crenulata, can be metabolized in different sites before being absorbed into systemic blood stream. This proposed an approach for describing biotransformation salidroside based on comparative analysis. In vitro incubation, situ closed-loop vivo sampling were used to determine relative contribution each site total...
Short peptide hydrogel was reported as a possible adjuvant for vaccines. In order to evaluate whether the Tetra-Peptide Hydrogel can be promising an H7N9 vaccine against highly pathogenic virus, we conducted this study.Tetra-Peptide Hydrogels (D and L conformations) were prepared by self-assembly system using Naproxen acid modified tetra of GFFY (Npx-GFFY). Mice received two immunizations with D-Tetra-Peptide vaccine, L-Tetra-Peptide or split vaccine. Fourteen days following second dose,...
Abstract Background Long non-coding RNAs (lncRNAs) are involved in the development of hepatocellular carcinoma (HCC). We aimed to investigate function LINC01146 HCC. Methods The expression HCC tissues was explored via Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases verified using quantitative real-time polymerase chain reaction (qRT–PCR) our cohort. Kaplan–Meier analysis used assess relationship between prognosis patients. Cell Counting Kit 8, colony formation assays,...
Lung cancers express the cholinergic autocrine loop, which facilitates progression of cancer cells. The antagonists mAChRs have been demonstrated to depress growth small cell lung (SCLCs). In this study we intended investigate inhibitory effect R2HBJJ, a novel muscarinic antagonist, on non-small (NSCLC) cells and possible mechanisms. competitive binding assay revealed that R2HBJJ had high affinity M3 M1 AChRs. presented strong anticholinergic activity carbachol-induced contraction guinea-pig...
Abstract Buddleja officinalis Maxim, one of the most popular herbal medicines in China, is widely prescribed for curing eye diseases centuries. In this study, major components B. extract (BOE) and their metabolites rat urine were detected identified by ultra‐high‐pressure liquid chromatography coupled with linear ion trap‐orbitrap tandem mass spectrometry (UHPLC‐LTQ‐Orbitrap). A total 19 compounds, including 8 flavonoids 11 phenylethanoid glycosides, confirmed or tentatively from BOE. vivo ,...
Neurotrophins are secreted proteins that control survival, differentiation, and synaptic plasticity. While mature neurotrophins regulate these functions via tyrosine kinase signaling (Trk), uncleaved pro-neurotrophins bind preferentially to the p75 neurotrophin receptor (p75NTR) often exert opposite effects those of neurotrophins. In amygdala, brain-derived neurotrophic factor (BDNF) enables long-term potentiation as well fear extinction learning. present study, we focused on impact BDNF...
Background: Growing evidence has demonstrated the role of ambient air pollutants in driving diabetes incidence. However, epidemiological linking ozone (O3) exposure to risk been scarcely studied Zhuang adults China. We aimed investigate associations long-term O3 with prevalence and fasting plasma glucose (FPG) estimate mediating liver enzymes adults. Methods: recruited 13 843 ethnic minority during 2018-2019 based on a cross-sectional study covering nine districts/counties Guangxi....
Abstract TLS-ERG fusion protein is derived from the t(16;21) translocation found in human myeloid leukemia. Here, we show that retroviral transduction of confers a growth advantage to L-G progenitor cells and blocks terminal differentiation. We level cyclin-dependent kinase 1 (Cdk1) was significantly decreased controls but unchanged TLS-ERG–expressing after granulocyte colony-stimulating factor treatment or interleukin-3 withdrawal. Injection induced rapid development leukemia-like disease...